LOCUS       sequence-225108-       10906 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control inducible vector with LacZ (V5 tagged).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10906)
  AUTHORS   Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, 
            Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed 
            A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, 
            Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare 
            B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, 
            Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, 
            Beroukhim R, Ligon KL, Boehm JS, Hahn WC
  TITLE     Renal medullary carcinomas depend upon SMARCB1 loss and are 
            sensitive to proteasome inhibition.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161.
  PUBMED    30860482
REFERENCE   2  (bases 1 to 10906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 44161. doi"; pages: " 
            10.7554/eLife.44161"
COMMENT     Sequence Label: pLXI_TRC401 LacZ
FEATURES             Location/Qualifiers
     source          1..10906
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        122..436
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    130..148
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    166..184
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    201..219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    237..255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    273..291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    308..326
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    344..362
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     409..433
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    457..481
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             496..3540
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(498..520)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     protein_bind    complement(3560..3584)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3637..3678
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    complement(3689..3713)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3731..3748
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        3758..4268
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4187..4205
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4278..4874
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4278..4297)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4774..4794
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             4875..4928
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4929..5546,5547..5552,5553..5588,5589..5591,5592..5627,
                     5628..5630,5631..5666,5667..5675)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(5715..5735)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6133..6144)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6349..6582
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6654..6775
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6691..6710)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6745..6764
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6815..6950
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        6950..7021
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7022..7090,7091..7882)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7240..7259)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8053..8641
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8542..8561
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8795..8812
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8929..8950
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8965..8970,8971..8988,8989..8995)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9003..9019
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9008..9030
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9027..9043
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9027..9043
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9062..9082
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9064..9082
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9110..9336
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9337..9517
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9564..9689
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10182..10415
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10600..10644
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    join(10857..10906,1..68)
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
       61 aaaattttcg ggtttattac agggacagca gagatccact ttggccgcga atcgatatgt
      121 cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata
      181 gagaacgatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttactccc
      241 tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag aacgtatgtc
      301 gagtttatcc ctatcagtga tagagaacgt atgtcgagtt tactccctat cagtgataga
      361 gaacgtatgt cgaggtaggc gtgtacggtg ggaggcctat ataagcagag ctcgtttagt
      421 gaaccgtcag atcgcctgga gaattggcta gcatcaacaa gtttgtacaa aaaagttggc
      481 accatgatag atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
      541 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
      601 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt
      661 ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact
      721 gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg
      781 acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac
      841 tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt
      901 gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag
      961 gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc
     1021 ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg
     1081 cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc
     1141 gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa
     1201 gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt
     1261 gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt
     1321 ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc
     1381 gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt
     1441 gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg
     1501 ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat
     1561 ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac
     1621 tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac
     1681 cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg
     1741 aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg
     1801 gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc
     1861 cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg
     1921 gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac
     1981 gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg
     2041 ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt
     2101 aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag
     2161 ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac
     2221 ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg
     2281 aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag
     2341 cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg
     2401 ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg
     2461 gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct
     2521 gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg
     2581 aacgcgaccg catggtcaga agccggccac atcagcgcct ggcagcagtg gcgtctggcg
     2641 gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc
     2701 gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc
     2761 tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag
     2821 ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct
     2881 aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg
     2941 cagtgcacgg cagatacact tgctgacgcg gtgctgatta cgaccgctca cgcgtggcag
     3001 catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa
     3061 atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc
     3121 ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa
     3181 gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca
     3241 gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa
     3301 ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt
     3361 caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg
     3421 ctgaatatcg acggtttcca tatggggatt ggtggagacg actcctggag cccgtcagta
     3481 tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa
     3541 actaagggtg ggcgcgccga cccagctttc ttgtacaaag tggttgatat ccagcacagt
     3601 ggcggccgct cgagtctaga gggcccgcgg ttcgaaggta agcctatccc taaccctctc
     3661 ctcggtctcg attctacgcg taccggtttg ccaactttct tgtacaaagt ggtttagtaa
     3721 tgaaccggtc caccaccacc accaccacta aggatccggg gttggggttg cgccttttcc
     3781 aaggcagccc tgggtttgcg cagggacgcg gctgctctgg gcgtggttcc gggaaacgca
     3841 gcggcgccga ccctgggtct cgcacattct tcacgtccgt tcgcagcgtc acccggatct
     3901 tcgccgctac ccttgtgggc cccccggcga cgcttcctgc tccgccccta agtcgggaag
     3961 gttccttgcg gttcgcggcg tgccggacgt gacaaacgga agccgcacgt ctcactagta
     4021 ccctcgcaga cggacagcgc cagggagcaa tggcagcgcg ccgaccgcga tgggctgtgg
     4081 ccaatagcgg ctgctcagca gggcgcgccg agagcagcgg ccgggaaggg gcggtgcggg
     4141 aggcggggtg tggggcggta gtgtgggccc tgttcctgcc cgcgcggtgt tccgcattct
     4201 gcaagcctcc ggagcgcacg tcggcagtcg gctccctcgt tgaccgaatc accgacctct
     4261 ctccccagca attcaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg
     4321 acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc
     4381 acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca
     4441 cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg
     4501 tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca
     4561 tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc
     4621 cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc
     4681 agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg
     4741 gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg
     4801 gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc
     4861 gcaagcccgg tgccgaaggt agaggttctc tcctcacttg tggtgatgtt gaagaaaacc
     4921 ctggtccaat gtctagactg gacaagagca aagtcataaa cggagctctg gaattactca
     4981 atggtgtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     5041 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     5101 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     5161 ttctgcggaa caacgccaag tcataccgct gtgctctcct ctcacatcgc gacggggcta
     5221 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     5281 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     5341 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     5401 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     5461 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     5521 agaaacagct aaagtgcgaa agcggcgggc cgaccgacgc ccttgacgat tttgacttag
     5581 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     5641 ttgacgattt tgaccttgac atgctccccg ggtaactaag taaggatcga tccaagatat
     5701 cgtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     5761 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     5821 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     5881 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     5941 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     6001 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg
     6061 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     6121 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     6181 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     6241 gcctccccgc ctgtttcgcc tcggcgtccg gactagaggt acctttaaga ccaatgactt
     6301 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     6361 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     6421 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     6481 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     6541 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc
     6601 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     6661 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     6721 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     6781 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     6841 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     6901 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tttcaaatat
     6961 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     7021 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     7081 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     7141 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     7201 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     7261 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     7321 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     7381 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     7441 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     7501 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     7561 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     7621 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     7681 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     7741 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     7801 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     7861 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     7921 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     7981 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     8041 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     8101 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     8161 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     8221 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     8281 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     8341 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     8401 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     8461 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     8521 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     8581 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     8641 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     8701 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     8761 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     8821 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     8881 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     8941 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     9001 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     9061 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     9121 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     9181 gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg tgccttatta
     9241 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     9301 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     9361 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9421 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9481 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     9541 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     9601 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     9661 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     9721 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     9781 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     9841 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     9901 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     9961 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
    10021 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
    10081 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
    10141 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
    10201 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
    10261 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    10321 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    10381 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    10441 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    10501 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    10561 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    10621 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    10681 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    10741 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    10801 ccaaccccga ggggacaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt
    10861 aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatag
//
