LOCUS       sequence-225115-        8872 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible vector (V5 tagged) with SMARCB1 variant 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, 
            Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed 
            A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, 
            Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare 
            B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, 
            Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, 
            Beroukhim R, Ligon KL, Boehm JS, Hahn WC
  TITLE     Renal medullary carcinomas depend upon SMARCB1 loss and are 
            sensitive to proteasome inhibition.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161.
  PUBMED    30860482
REFERENCE   2  (bases 1 to 8872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 44161. doi"; pages: " 
            10.7554/eLife.44161"
COMMENT     Sequence Label: pLXI_TRC403 SMARCB1 var 2
FEATURES             Location/Qualifiers
     source          1..8872
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..354
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    48..66
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    84..102
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    119..137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     327..351
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    375..399
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1531..1555)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1560..1601
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             1615..1632
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        1642..2152
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2071..2089
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2162..2758
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2162..2181)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2658..2678
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2759..2812
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2813..3430,3431..3436,3437..3472,3473..3475,3476..3511,
                     3512..3514,3515..3550,3551..3559)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3599..3619)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4017..4028)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4233..4466
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4538..4659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4575..4594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4629..4648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4699..4834
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4834..4905
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4906..4974,4975..5766)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5124..5143)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5937..6525
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6426..6445
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6679..6696
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6813..6834
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6849..6854,6855..6872,6873..6879)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6887..6903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6892..6914
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6911..6927
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6911..6927
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6946..6966
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6948..6966
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6994..7220
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7221..7401
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7448..7573
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8066..8299
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8484..8528
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8741..8858
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggacagcaga gatccacttt ggccgcgaat cgatatgtcg agtttactcc ctatcagtga
       61 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata
      241 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt
      301 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
      361 attggctagc atcaacaagt ttgtacaaaa aagttggcac catgatgatg atggcgctga
      421 gcaagacctt cgggcagaag cccgtgaagt tccagctgga ggacgacggc gagttctaca
      481 tgatcggctc cgaggtggga aactacctcc gtatgttccg aggttctctg tacaagagat
      541 acccctcact ctggaggcga ctagccactg tggaagagag gaagaaaata gttgcatcgt
      601 cacatgatca cggatacacg actctagcca ccagtgtgac cctgttaaaa gcctcggaag
      661 tggaagagat tctggatggc aacgatgaga agtacaaggc tgtgtccatc agcacagagc
      721 cccccaccta cctcagggaa cagaaggcca agaggaacag ccagtgggta cccaccctgc
      781 ccaacagctc ccaccactta gatgccgtgc catgctccac aaccatcaac aggaaccgca
      841 tgggccgaga caagaagaga accttccccc tttgctttga tgaccatgac ccagctgtga
      901 tccatgagaa cgcatctcag cccgaggtgc tggtccccat ccggctggac atggagatcg
      961 atgggcagaa gctgcgagac gccttcacct ggaacatgaa tgagaagttg atgacgcctg
     1021 agatgttttc agaaatcctc tgtgacgatc tggatttgaa cccgctgacg tttgtgccag
     1081 ccatcgcctc tgccatcaga cagcagatcg agtcctaccc cacggacagc atcctggagg
     1141 accagtcaga ccagcgcgtc atcatcaagc tgaacatcca tgtgggaaac atttccctgg
     1201 tggaccagtt tgagtgggac atgtcagaga aggagaactc accagagaag tttgccctga
     1261 agctgtgctc ggagctgggg ttgggcgggg agtttgtcac caccatcgca tacagcatcc
     1321 ggggacagct gagctggcat cagaagacct acgccttcag cgagaaccct ctgcccacag
     1381 tggagattgc catccggaac acgggcgatg cggaccagtg gtgcccactg ctggagactc
     1441 tgacagacgc tgagatggag aagaagatcc gcgaccagga caggaacacg aggcggatga
     1501 ggcgtcttgc caacacggcc ccggcctggt tgccaacttt cttgtacaaa gtggttgcag
     1561 gaaagccaat cccaaaccca ctcctaggtc tcgacagtac agcatagacc ggtccaccac
     1621 caccaccacc actaaggatc cggggttggg gttgcgcctt ttccaaggca gccctgggtt
     1681 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     1741 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     1801 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     1861 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
     1921 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
     1981 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
     2041 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
     2101 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agcaattcac
     2161 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     2221 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     2281 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     2341 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     2401 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     2461 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     2521 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     2581 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     2641 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     2701 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgccga
     2761 aggtagaggt tctctcctca cttgtggtga tgttgaagaa aaccctggtc caatgtctag
     2821 actggacaag agcaaagtca taaacggagc tctggaatta ctcaatggtg tcggtatcga
     2881 aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg
     2941 gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca
     3001 tcatacccac ttctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc
     3061 caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac
     3121 ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca
     3181 aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg
     3241 ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac
     3301 cgattctatg cccccacttc tgagacaagc aattgagctg ttcgaccggc agggagccga
     3361 acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg
     3421 cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga
     3481 tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct
     3541 tgacatgctc cccgggtaac taagtaagga tcgatccaag atatcgtatt cttaactatg
     3601 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     3661 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     3721 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     3781 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     3841 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     3901 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     3961 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     4021 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     4081 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgttt
     4141 cgcctcggcg tccggactag aggtaccttt aagaccaatg acttacaagg cagctgtaga
     4201 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     4261 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     4321 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     4381 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     4441 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     4501 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     4561 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     4621 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     4681 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     4741 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     4801 ttccagaagt agtgaggagg cttttttgga ggcctttcaa atatgtatcc gctcatgaga
     4861 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4921 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4981 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     5041 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     5101 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     5161 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     5221 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     5281 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5341 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5401 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5461 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5521 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5581 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5641 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5701 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5761 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5821 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5881 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5941 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     6001 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     6061 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     6121 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     6181 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     6241 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6301 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6361 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6421 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6481 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6541 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     6601 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     6661 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     6721 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     6781 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     6841 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     6901 caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac
     6961 taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt
     7021 cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg
     7081 catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg
     7141 gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt
     7201 gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct
     7261 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7321 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7381 cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc
     7441 agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg
     7501 gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg
     7561 gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt
     7621 aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct
     7681 agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact
     7741 gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac
     7801 agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt
     7861 agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga
     7921 tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata
     7981 aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc
     8041 agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag
     8101 gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg
     8161 gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc
     8221 aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc
     8281 taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg
     8341 ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga
     8401 cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg
     8461 aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg
     8521 caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa
     8581 tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata
     8641 gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac
     8701 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     8761 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     8821 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac ag
//
