LOCUS       sequence-225120-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  single guide RNA targeting UBE2C; guide 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, 
            Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed 
            A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, 
            Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare 
            B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, 
            Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, 
            Beroukhim R, Ligon KL, Boehm JS, Hahn WC
  TITLE     Renal medullary carcinomas depend upon SMARCB1 loss and are 
            sensitive to proteasome inhibition.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161.
  PUBMED    30860482
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 44161. doi"; pages: " 
            10.7554/eLife.44161"
COMMENT     Sequence Label: pXPR_BRD003 UBE2C guide 2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        206..454
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     206..226
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     377..396
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        476..551
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    607..724
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        863..2041
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1094..2032
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1989..2009
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2054..2653
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2054..2073)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2550..2570
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2669..3257
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2722..2742)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3140..3151)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3329..3562
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3640..3761
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3677..3696)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3731..3750
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3801..3936
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3956..3975)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3957..3975)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3985..4002)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3985..4001)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3994..4016)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4143..4598
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4230..4249)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4440..4461
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4624..4728
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4729..4797,4798..5589)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4947..4966)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5760..6348
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6249..6268
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6502..6519
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6636..6657
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6672..6677,6678..6695,6696..6702)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6710..6726
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6715..6737
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6734..6750
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6734..6750
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6769..6789
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6771..6789
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6817..7043
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7044..7224
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7271..7396
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7889..8122
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             join(8307..8323,1..28)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
       61 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
      121 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
      181 cctcccaacc ccgaggggac ccagagaggg cctatttccc atgattcctt catatttgca
      241 tatacgatac aaggctgtta gagagataat tagaattaat ttgactgtaa acacaaagat
      301 attagtacaa aatacgtgac gtagaaagta ataatttctt gggtagtttg cagttttaaa
      361 attatgtttt aaaatggact atcatatgct taccgtaact tgaaagtatt tcgatttctt
      421 ggctttatat atcttgtgga aaggacgaaa caccggggcg tgaggaactt cactggtttt
      481 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac
      541 cgagtcggtg cttttttaag cttggcgtaa ctagatcttg agacaaatgg cagtattcat
      601 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
      661 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
      721 ttttcgggtt tattacaggg acagcagaga tccactttgg cgccggctcg agggggcccg
      781 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
      841 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
      901 cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg
      961 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     1021 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     1081 cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta
     1141 tggcccttgc gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga
     1201 gcttcgggtt ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc
     1261 tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca
     1321 ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc
     1381 tgctgcgacg ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac
     1441 tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg
     1501 ttcggcgagg cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc
     1561 tggccggcct gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc
     1621 aaggctggcc cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc
     1681 tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac
     1741 acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg
     1801 ggcgccgtcc aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg
     1861 gggggagggg ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag
     1921 gccagcttgg cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg
     1981 gttcattctc aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg
     2041 acgtacggcc accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     2101 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     2161 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     2221 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     2281 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     2341 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     2401 ccggcccaag gagcccgcgt ggttcctggc caccgtcgga gtctcgcccg accaccaggg
     2461 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     2521 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     2581 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     2641 gcccggtgcc tgaacgcgtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     2701 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     2761 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     2821 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     2881 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     2941 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     3001 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     3061 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     3121 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     3181 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     3241 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     3301 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     3361 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     3421 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     3481 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     3541 tcagtgtgga aaatctctag cagtacgtat agtagttcat gtcatcttat tattcagtat
     3601 ttataacttg caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat
     3661 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3721 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta
     3781 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3841 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     3901 tattccagaa gtagtgagga ggcttttttg gaggcctagg gacgtaccca attcgcccta
     3961 tagtgagtcg tattacgcgc gctcactggc cgtcgtttta caacgtcgtg actgggaaaa
     4021 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     4081 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     4141 ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     4201 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     4261 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     4321 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     4381 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     4441 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     4501 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     4561 taacgcgaat tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat
     4621 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4681 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4741 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4801 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4861 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4921 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     4981 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5041 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5101 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5161 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5221 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5281 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5341 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5401 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5461 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5521 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5581 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5641 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5701 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5761 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5821 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5881 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     5941 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6001 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6061 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6121 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6181 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6241 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6301 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6361 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6421 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6481 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6541 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6601 cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag
     6661 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga
     6721 taacaatttc acacaggaaa cagctatgac catgattacg ccaagcgcgc aattaaccct
     6781 cactaaaggg aacaaaagct ggagctgcaa gcttaatgta gtcttatgca atactcttgt
     6841 agtcttgcaa catggtaacg atgagttagc aacatgcctt acaaggagag aaaaagcacc
     6901 gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc cttattagga aggcaacaga
     6961 cgggtctgac atggattgga cgaaccactg aattgccgca ttgcagagat attgtattta
     7021 agtgcctagc tcgatacata aacgggtctc tctggttaga ccagatctga gcctgggagc
     7081 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7141 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7201 agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa
     7261 accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga
     7321 ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga
     7381 tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg
     7441 gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga
     7501 gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat
     7561 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
     7621 tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc
     7681 tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc
     7741 tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat
     7801 ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg
     7861 tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag
     7921 caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt
     7981 ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt
     8041 tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat
     8101 acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca
     8161 ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca
     8221 cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa
     8281 ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga att
//
