LOCUS       sequence-225122-       16381 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NuMA(1-2115)-RFP-Nano from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16381)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 16381)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK715
FEATURES             Location/Qualifiers
     source          1..16381
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1172..1253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1370..2116)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2135..2645)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2665..2713
                     /note="synthetic polyadenylation signal"
     misc_feature    2727..2818
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2767..2786
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2822..3189
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2830..2848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2866..2884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2902..2920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3046..3064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      9591..9600
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9597..10328
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             10404..10733
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    10883..10964
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        10986..11496
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             11533..12552
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             11533..12552
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(12862..12879)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12868..13092
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(14193..14213)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14193..14211)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14232..14248)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14232..14248)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14245..14267)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14256..14272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14280..14286,14287..14304,14305..14310))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14325..14346
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(14634..15222)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14714..14733)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15393..16184,16185..16253))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16016..16035
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16254..16358)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gaagctgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt atttgtgaaa
     1201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1261 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1321 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagatt tacccgggga
     1381 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
     1441 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
     1501 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
     1561 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
     1621 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
     1681 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
     1741 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
     1801 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
     1861 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
     1921 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
     1981 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
     2041 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
     2101 tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt
     2161 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2221 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2281 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2341 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2401 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2461 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     2521 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     2581 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     2641 accccgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     2701 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     2761 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     2821 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2881 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2941 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3001 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3061 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     3121 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     3181 cctcgtaaag aattcgcggc cgcacgcgtg ccaccatgac actccacgcc acccgggggg
     3241 ctgcactcct ctcttgggtg aacagtctac acgtggctga ccctgtggag gctgtgctgc
     3301 agctccagga ctgcagcatc ttcatcaaga tcattgacag aatccatggc actgaagagg
     3361 gacagcaaat cttgaagcag ccggtgtcag agagactgga ctttgtgtgc agttttctgc
     3421 agaaaaatcg aaaacatccc tcttccccag aatgcctggt atctgcacag aaggtgctag
     3481 agggatcaga gctggaactg gcgaagatga ccatgctgct cttataccac tctaccatga
     3541 gctccaaaag tcccagggac tgggaacagt ttgaatataa aattcaggct gagttggctg
     3601 tcattcttaa atttgtgctg gaccatgagg acgggctaaa ccttaatgag gacctagaga
     3661 acttcctaca gaaagctcct gtgccttcta cctgttctag cacattccct gaagagctct
     3721 ccccacctag ccaccaggcc aagagggaga ttcgcttcct agagctacag aaggttgcct
     3781 cctcttccag tgggaacaac tttctctcag gttctccagc ttctcccatg ggtgatatcc
     3841 tgcagacccc acagttccag atgagacggc tgaagaagca gcttgctgat gagagaagta
     3901 atagggatga gctggagctg gagctagctg agaaccgcaa gctcctcacc gagaaggatg
     3961 cacagatagc catgatgcag cagcgcattg accgcctagc cctgctgaat gagaagcagg
     4021 cggccagccc actggagccc aaggagcttg aggagctgcg tgacaagaat gagagcctta
     4081 ccatgcggct gcatgaaacc ctgaagcagt gccaggacct gaagacagag aagagccaga
     4141 tggatcgcaa aatcaaccag ctttcggagg agaatggaga cctttccttt aagctgcggg
     4201 agtttgccag tcatctgcag cagctacagg atgccctcaa tgagctgacg gaggagcaca
     4261 gcaaggccac tcaggagtgg ctagagaagc aggcccagct ggagaaggag ctcagcgcag
     4321 ccctgcagga caagaaatgc cttgaagaga agaacgaaat ccttcaggga aaactttcac
     4381 agctggaaga acacttgtcc cagctgcagg ataacccacc ccaggagaag ggcgaggtgc
     4441 tgggtgatgt cttgcagctg gaaaccttga agcaagaggc agccactctt gctgcaaaca
     4501 acacacagct ccaagccagg gtagagatgc tggagactga gcgaggccag caggaagcca
     4561 agctgcttgc tgagcggggc cacttcgaag aagaaaagca gcagctgtct agcctgatca
     4621 ctgacctgca gagctccatc tccaacctca gccaggccaa ggaagagctg gagcaggcct
     4681 cccaggctca tggggcccgg ttgactgccc aggtggcctc tctgacctct gagctcacca
     4741 cactcaatgc caccatccag caacaggatc aagaactggc tggcctgaag cagcaggcca
     4801 aagagaagca ggcccagcta gcacagaccc tccaacagca agaacaggcc tcccagggcc
     4861 tccgccacca ggtggagcag ctaagcagta gcctgaagca gaaggagcag cagttgaagg
     4921 aggtagcgga gaagcaggag gcaactaggc aggaccatgc ccagcaactg gccactgctg
     4981 cagaggagcg agaggcctcc ttaagggagc gggatgcggc tctcaagcag ctggaggcac
     5041 tggagaagga gaaggctgcc aagctggaga ttctgcagca gcaacttcag gtggctaatg
     5101 aagcccggga cagtgcccag acctcagtga cacaggccca gcgggagaag gcagagctga
     5161 gccggaaggt ggaggaactc caggcctgtg ttgagacagc ccgccaggaa cagcatgagg
     5221 cccaggccca ggttgcagag ctagagttgc agctgcggtc tgagcagcaa aaagcaactg
     5281 agaaagaaag ggtggcccag gagaaggacc agctccagga gcagctccag gccctcaaag
     5341 agtccttgaa ggtcaccaag ggcagccttg aagaggagaa gcgcagggct gcagatgccc
     5401 tggaagagca gcagcgttgt atctctgagc tgaaggcaga gacccgaagc ctggtggagc
     5461 agcataagcg ggaacgaaag gagctggaag aagagagggc tgggcgcaag gggctggagg
     5521 ctcgattaca gcagcttggg gaggcccatc aggctgagac tgaagtcctg cggcgggagc
     5581 tggcagaggc catggctgcc cagcacacag ctgagagtga gtgtgagcag ctcgtcaaag
     5641 aagtagctgc ctggcgtgag cggtatgagg atagccagca agaggaggca cagtatggcg
     5701 ccatgttcca ggaacagctg atgactttga aggaggaatg tgagaaggcc cgccaggagc
     5761 tgcaggaggc aaaggagaag gtggcaggca tagaatccca cagcgagctc cagataagcc
     5821 ggcagcagaa caaactagct gagctccatg ccaacctggc cagagcactc cagcaggtcc
     5881 aagagaagga agtcagggcc cagaagcttg cagatgacct ctccactctg caggaaaaga
     5941 tggctgccac cagcaaagag gtggcccgct tggagacctt ggtgcgcaag gcaggtgagc
     6001 agcaggaaac agcctcccgg gagttagtca aggagcctgc gagggcagga gacagacagc
     6061 ccgagtggct ggaagagcaa cagggacgcc agttctgcag cacacaggca gcgctgcagg
     6121 ctatggagcg ggaggcagag cagatgggca atgagctgga acggctgcgg gccgcgctga
     6181 tggagagcca ggggcagcag caggaggagc gtgggcagca ggaaagggag gtggcgcggc
     6241 tgacccagga gcggggccgt gcccaggctg accttgccct ggagaaggcg gccagagcag
     6301 agcttgagat gcggctgcag aacgccctca acgagcagcg tgtggagttc gctaccctgc
     6361 aagaggcact ggctcatgcc ctgacggaaa aggaaggcaa ggaccaggag ttggccaagc
     6421 ttcgtggtct ggaggcagcc cagataaaag agctggagga acttcggcaa accgtgaagc
     6481 aactgaagga acagctggct aagaaagaaa aggagcacgc atctggctca ggagcccaat
     6541 ctgaggctgc tggcaggaca gagccaacag gccccaagct ggaagcactg cgggcagagg
     6601 tgagcaagct ggaacagcaa tgccagaagc agcaggagca ggctgacagc ctggaacgca
     6661 gcctcgaggc tgagcgggcc tcccgggctg agcgggacag tgctctggag actctgcagg
     6721 gccagttaga ggagaaggcc caggagctag ggcacagtca gagtgcctta gcctcggccc
     6781 aacgggagtt ggctgccttc cgcaccaagg tacaagacca cagcaaggct gaagatgagt
     6841 ggaaggccca ggtggcccgg ggccggcaag aggctgagag gaaaaatagc ctcatcagca
     6901 gcttggagga ggaggtgtcc atcctgaatc gccaggtcct ggagaaggag ggggagagca
     6961 aggagttgaa gcggctggtg atggccgagt cagagaagag ccagaagctg gaggagaggc
     7021 tgcgcctgct gcaggcagag acagccagca acagtgccag agctgcagaa cgcagctctg
     7081 ctctgcggga ggaggtgcag agcctccggg aggaggctga gaaacagcgg gtggcttcag
     7141 agaacctgcg gcaggagctg acctcacagg ctgagcgtgc ggaggagctg ggccaagaat
     7201 tgaaggcgtg gcaggagaag ttcttccaga aagagcaggc cctctccacc ctgcagctcg
     7261 agcacaccag cacacaggcc ctggtgagtg agctgctgcc agctaagcac ctctgccagc
     7321 agctgcaggc cgagcaggcc gctgccgaga aacgccaccg tgaggagctg gagcagagca
     7381 agcaggccgc tgggggactg cgggcagagc tgctgcgggc ccagcgggag cttggggagc
     7441 tgattcctct gcggcagaag gtggcagagc aggagcgaac agctcagcag ctgcgggcag
     7501 agaaggccag ctatgcagag cagctgagca tgctgaagaa ggcgcatggc ctgctggcag
     7561 aggagaaccg ggggctgggt gagcgggcca accttggccg gcagtttctg gaagtggagt
     7621 tggaccaggc ccgggaaaag tatgtccaag agttggcagc cgtacgtgct gatgctgaga
     7681 cccgtctggc tgaggtgcag cgagaagcac agagcactgc ccgggagctg gaggtgatga
     7741 ctgccaagta tgagggtgcc aaggtcaagg tcctggagga gaggcagcgg ttccaggaag
     7801 agaggcagaa actcactgcc caggkggaag rgttccagga agagaggcag aaactcactg
     7861 cccaggtgga agaactgagt aagaaactgg ctgactctga ccaagccagc aaggtgcagc
     7921 agcagaagct gaaggctgtc caggctcagg gaggcgagag ccagcaggag gcccagcgct
     7981 tccaggccca gctgaatgaa ctgcaagccc agttgagcca gaaggagcag gcagctgagc
     8041 actataagct gcagatggag aaagccaaaa cacattatga tgccaagaag cagcagaacc
     8101 aagagctgca ggagcagctg cggagcctgg agcagctgca gaaggaaaac aaagagctgc
     8161 gagctgaagc tgaacggctg ggccatgagc tacagcaggc tgggctgaag accaaggagg
     8221 ctgaacagac ctgccgccac cttactgccc aggtgcgcag cctggaggca caggttgccc
     8281 atgcagacca gcagcttcga gacctgggca aattccaggt ggcaactgat gctttaaaga
     8341 gccgtgagcc ccaggctaag ccccagctgg acttgagtat tgacagcctg gatctgagct
     8401 gcgaggaggg gaccccactc agtatcacca gcaagctgcc tcgtacccag ccagacggca
     8461 ccagcgtccc tggagaacca gcctcaccta tctcccagcg cctgcccccc aaggtagaat
     8521 ccctggagag tctctacttc actcccatcc ctgctcggag tcaggccccc ctggagagca
     8581 gcctggactc cctgggagac gtcttcctgg actcgggtcg taagacccgc tccgctcgtc
     8641 ggcgcaccac gcagatcatc aacatcacca tgaccaagaa gctagatgtg gaagagccag
     8701 acagcgccaa ctcatcgttc tacagcacgc ggtctgctcc tgcttcccag gctagcctgc
     8761 gagccacctc ctctactcag tctctagctc gcctgggttc tcccgattat ggcaactcag
     8821 ccctgctcag cttgcctggc taccgcccca ccactcgcag ttctgctcgt cgttcccagg
     8881 ccggggtgtc cagtggggcc cctccaggaa ggaacagctt ctacatgggc acttgccagg
     8941 atgagcctga gcagctggat gactggaacc gcattgcaga gctgcagcag cgcaatcgag
     9001 tgtgcccccc acatctgaag acctgctatc ccctggagtc caggccttcc ctgagcctgg
     9061 gcaccatcac agatgaggag atgaaaactg gagaccccca agagaccctg cgccgagcca
     9121 gcatgcagcc aatccagata gccgagggca ctggcatcac cacccggcag cagcgcaaac
     9181 gggtctccct agagccccac cagggccctg gaactcctga gtctaagaag gccaccagct
     9241 gtttcccacg ccccatgact ccccgagacc gacatgaagg gcgcaaacag agcactactg
     9301 aggcccagaa gaaagcagct ccagcttcta ctaaacaggc tgaccggcgc cagtcgatgg
     9361 ccttcagcat cctcaacaca cccaagaagc tagggaacag ccttctgcgg cggggagcct
     9421 caaagaaggc cctgtccaag gcttccccca acacycgcag tggaacccgc cgttctccgc
     9481 gcattgccac caccacagcc agtgccgcca ctgctgccgc cattggtgcc acccctcgag
     9541 ccaagggcaa ggcaaagcac ggaggtggat ctacgcgtgg aggtggatct gccaccatgg
     9601 tgtctaaggg cgaagagctg attaaggaga acatgcacat gaagctgtac atggagggca
     9661 ccgtgaacaa ccaccacttc aagtgcacat ccgagggcga aggcaagccc tacgagggca
     9721 cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc ttcgacatcc
     9781 tggctaccag cttcatgtac ggcagcagaa ccttcatcaa ccacacccag ggcatccccg
     9841 acttctttaa gcagtccttc cctgagggct tcacatggga gagagtcacc acatacgaag
     9901 acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc ctcatctaca
     9961 acgtcaagat cagaggggtg aacttcccat ccaacggccc tgtgatgcag aagaaaacac
    10021 tcggctggga ggccaacacc gagatgctgt accccgctga cggcggcctg gaaggcagaa
    10081 ccgacatggc cctgaagctc gtgggcgggg gccacctgat ctgcaacttc aagaccacat
    10141 acagatccaa gaaacccgct aagaacctca agatgcccgg cgtctactat gtggaccaca
    10201 gactggaaag aatcaaggag gccgacaaag agacctacgt cgagcagcac gaggtggctg
    10261 tggccagata ctgcgacctc cctagcaaac tggggcacaa acttaatggc atggacgagc
    10321 tgtacaagtc cggactcaga tctcgagctc aagcttcgaa cgaattcggc attgatctga
    10381 gcggcctgac cctgcaggaa ttcagctccc cgaaacgccc taagctgctg cgtgaatatt
    10441 acgattggct ggttgataac agctttaccc catatctggt ggtggatgcc acatacctgg
    10501 gcgtgaacgt gcccgtggag tatgtgaaag acggtcagat cgtgctgaat ctgtctgcaa
    10561 gtgcgaccgg caacctgcaa ctgacaaatg attttatcca gttcaacgcc cgctttaagg
    10621 gcgtgtctcg tgaactgtat atcccgatgg gtgccgctct ggccatttac gctcgcgaga
    10681 acggcgatgg tgtgatgttc gaaccagaag aaatctatga cgagctgaat attggttaaa
    10741 ccggtggatc cagatctctg cagcatatgt ctagaggatc ataatcagcc ataccacatt
    10801 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
    10861 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
    10921 caatagcatc acaaatttca caaataaagc atttttttca ctgccgacgt caggtggcac
    10981 ttttcggggt tggggttgcg ccttttccaa ggcagccctg ggtttgcgca gggacgcggc
    11041 tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg cacattcttc
    11101 acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc cccggcgacg
    11161 cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg ccggacgtga
    11221 caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca gggagcaatg
    11281 gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg gcgcgccgag
    11341 agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt gtgggccctg
    11401 ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc ggcagtcggc
    11461 tccctcgttg accgaatcac cgacctctct ccccaggggg atccaccgga gcttaccatg
    11521 gatagatccg gaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
    11581 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
    11641 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
    11701 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
    11761 gacattgggg aattcagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
    11821 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
    11881 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
    11941 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
    12001 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
    12061 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
    12121 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
    12181 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
    12241 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
    12301 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
    12361 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
    12421 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
    12481 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
    12541 gcaaaggaat agagtagatg ccgaccgaac aagagctgat ttcgagaacg cctcagccag
    12601 caactcgcgc gagcctagca aggcaaatgc gagagaacgg ccttacgctt ggtggcacag
    12661 ttctcgtcca cagttcgcta agctcgctcg gctgggtcgc gggagggccg gtcgcagtga
    12721 ttcaggccct tctggattgt gttggtcccc agggcacgat tgtcatgccc acgcactcgg
    12781 gtgatctgac tgatcccgca gattggagat cgccgcccgt gcctgccgat tgggtgcaga
    12841 tctctagagc tcgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
    12901 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
    12961 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
    13021 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
    13081 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctcgatgagg gagctgcggt
    13141 ggaggaggtg gagagaaggc cgcacccttc tgggcagggg gaggggagtg ccgcaatacc
    13201 tttatgggag ttctctgctg cctcccgtct tgtaaggacc gccctgggcc tggaagaagc
    13261 cctccctcct ttcctcctcg cgtgatctcg tcatcgcctc catgtcgagt cgcttctcga
    13321 ttatgggcgg gattcttttg cctaggctta aggggctaac ttggtccctg ggcgttgccc
    13381 tgcaggggag tgagcagctg taagatttga ggggcgactc cgattagttt atcttcccac
    13441 ggactagagt tggtgtcgag gttattgtaa taagggtggg gtagggaaat ggagcttagt
    13501 cattcacctg gggctgattt tatgcaacga gactgcggat tatcactaat catttttgga
    13561 gcatttttct agagacagac ataaagcatg atcacctgag ttttatacca tttgagaccc
    13621 ttgctgcacc accaaagtgt agcatcaggt taaatcttaa tagaaaaatt ttagcttttg
    13681 cttgagaaac cagtgcttcc ctccctcacc ctctctcccc aggctctcta cccctttgca
    13741 tccctaccag gcatcttagc aactctcact catacttgat cccattttcc atttgttgta
    13801 cttgctcctc cagtattcag acatagcact agctttctcc ctctcttgat cttgggtagc
    13861 ctggtgtctc gcgaaaccag acagattggt tccaccacaa attaaggctt gagctggggc
    13921 ttgactctta cccagcagtg cttttattcc tccctagttc acgttcttaa atgtttatct
    13981 tgattttcat tttatccttt ttccttagct gggattctgt ccctgaccgt cttcacagtc
    14041 caggtgatct tgactactgc tttacagaga attggatctg aggttaggca acatctccct
    14101 ttttcttcct ctaaatacct ctcatttctg ttcttaccag ttagtaactg atctcagatg
    14161 cctgtgtgat agctggtacc cagcttttgt tccctttagt gagggttaat tgcgcgcttg
    14221 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
    14281 aacatacgag ccgggagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
    14341 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
    14401 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
    14461 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
    14521 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
    14581 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
    14641 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
    14701 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
    14761 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
    14821 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
    14881 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
    14941 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
    15001 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
    15061 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
    15121 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
    15181 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
    15241 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
    15301 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
    15361 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
    15421 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
    15481 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
    15541 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
    15601 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
    15661 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
    15721 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
    15781 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
    15841 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
    15901 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
    15961 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
    16021 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
    16081 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
    16141 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    16201 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    16261 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    16321 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    16381 c
//
