LOCUS       sequence-225127-       16366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NuMA(1-2115-Delta-NLS)-RFP-Nano from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16366)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 16366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK713
FEATURES             Location/Qualifiers
     source          1..16366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1172..1253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1370..2116)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2135..2645)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2665..2713
                     /note="synthetic polyadenylation signal"
     misc_feature    2727..2818
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2767..2786
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2822..3189
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2830..2848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2866..2884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2902..2920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3046..3064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      9576..9585
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9582..10313
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             10389..10718
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    10868..10949
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        10971..11481
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             11518..12537
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             11518..12537
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(12847..12864)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12853..13077
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(14178..14198)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(14178..14196)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(14217..14233)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(14217..14233)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(14230..14252)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    14241..14257
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(14265..14271,14272..14289,14290..14295))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14310..14331
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(14619..15207)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(14699..14718)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(15378..16169,16170..16238))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     16001..16020
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(16239..16343)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gaagctgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt atttgtgaaa
     1201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1261 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1321 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagatt tacccgggga
     1381 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
     1441 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
     1501 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
     1561 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
     1621 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
     1681 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
     1741 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
     1801 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
     1861 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
     1921 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
     1981 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
     2041 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
     2101 tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt
     2161 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2221 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2281 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2341 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2401 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2461 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     2521 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     2581 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     2641 accccgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     2701 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     2761 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     2821 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2881 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2941 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3001 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3061 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     3121 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     3181 cctcgtaaag aattcgcggc cgcacgcgtg ccaccatgac actccacgcc acccgggggg
     3241 ctgcactcct ctcttgggtg aacagtctac acgtggctga ccctgtggag gctgtgctgc
     3301 agctccagga ctgcagcatc ttcatcaaga tcattgacag aatccatggc actgaagagg
     3361 gacagcaaat cttgaagcag ccggtgtcag agagactgga ctttgtgtgc agttttctgc
     3421 agaaaaatcg aaaacatccc tcttccccag aatgcctggt atctgcacag aaggtgctag
     3481 agggatcaga gctggaactg gcgaagatga ccatgctgct cttataccac tctaccatga
     3541 gctccaaaag tcccagggac tgggaacagt ttgaatataa aattcaggct gagttggctg
     3601 tcattcttaa atttgtgctg gaccatgagg acgggctaaa ccttaatgag gacctagaga
     3661 acttcctaca gaaagctcct gtgccttcta cctgttctag cacattccct gaagagctct
     3721 ccccacctag ccaccaggcc aagagggaga ttcgcttcct agagctacag aaggttgcct
     3781 cctcttccag tgggaacaac tttctctcag gttctccagc ttctcccatg ggtgatatcc
     3841 tgcagacccc acagttccag atgagacggc tgaagaagca gcttgctgat gagagaagta
     3901 atagggatga gctggagctg gagctagctg agaaccgcaa gctcctcacc gagaaggatg
     3961 cacagatagc catgatgcag cagcgcattg accgcctagc cctgctgaat gagaagcagg
     4021 cggccagccc actggagccc aaggagcttg aggagctgcg tgacaagaat gagagcctta
     4081 ccatgcggct gcatgaaacc ctgaagcagt gccaggacct gaagacagag aagagccaga
     4141 tggatcgcaa aatcaaccag ctttcggagg agaatggaga cctttccttt aagctgcggg
     4201 agtttgccag tcatctgcag cagctacagg atgccctcaa tgagctgacg gaggagcaca
     4261 gcaaggccac tcaggagtgg ctagagaagc aggcccagct ggagaaggag ctcagcgcag
     4321 ccctgcagga caagaaatgc cttgaagaga agaacgaaat ccttcaggga aaactttcac
     4381 agctggaaga acacttgtcc cagctgcagg ataacccacc ccaggagaag ggcgaggtgc
     4441 tgggtgatgt cttgcagctg gaaaccttga agcaagaggc agccactctt gctgcaaaca
     4501 acacacagct ccaagccagg gtagagatgc tggagactga gcgaggccag caggaagcca
     4561 agctgcttgc tgagcggggc cacttcgaag aagaaaagca gcagctgtct agcctgatca
     4621 ctgacctgca gagctccatc tccaacctca gccaggccaa ggaagagctg gagcaggcct
     4681 cccaggctca tggggcccgg ttgactgccc aggtggcctc tctgacctct gagctcacca
     4741 cactcaatgc caccatccag caacaggatc aagaactggc tggcctgaag cagcaggcca
     4801 aagagaagca ggcccagcta gcacagaccc tccaacagca agaacaggcc tcccagggcc
     4861 tccgccacca ggtggagcag ctaagcagta gcctgaagca gaaggagcag cagttgaagg
     4921 aggtagcgga gaagcaggag gcaactaggc aggaccatgc ccagcaactg gccactgctg
     4981 cagaggagcg agaggcctcc ttaagggagc gggatgcggc tctcaagcag ctggaggcac
     5041 tggagaagga gaaggctgcc aagctggaga ttctgcagca gcaacttcag gtggctaatg
     5101 aagcccggga cagtgcccag acctcagtga cacaggccca gcgggagaag gcagagctga
     5161 gccggaaggt ggaggaactc caggcctgtg ttgagacagc ccgccaggaa cagcatgagg
     5221 cccaggccca ggttgcagag ctagagttgc agctgcggtc tgagcagcaa aaagcaactg
     5281 agaaagaaag ggtggcccag gagaaggacc agctccagga gcagctccag gccctcaaag
     5341 agtccttgaa ggtcaccaag ggcagccttg aagaggagaa gcgcagggct gcagatgccc
     5401 tggaagagca gcagcgttgt atctctgagc tgaaggcaga gacccgaagc ctggtggagc
     5461 agcataagcg ggaacgaaag gagctggaag aagagagggc tgggcgcaag gggctggagg
     5521 ctcgattaca gcagcttggg gaggcccatc aggctgagac tgaagtcctg cggcgggagc
     5581 tggcagaggc catggctgcc cagcacacag ctgagagtga gtgtgagcag ctcgtcaaag
     5641 aagtagctgc ctggcgtgag cggtatgagg atagccagca agaggaggca cagtatggcg
     5701 ccatgttcca ggaacagctg atgactttga aggaggaatg tgagaaggcc cgccaggagc
     5761 tgcaggaggc aaaggagaag gtggcaggca tagaatccca cagcgagctc cagataagcc
     5821 ggcagcagaa caaactagct gagctccatg ccaacctggc cagagcactc cagcaggtcc
     5881 aagagaagga agtcagggcc cagaagcttg cagatgacct ctccactctg caggaaaaga
     5941 tggctgccac cagcaaagag gtggcccgct tggagacctt ggtgcgcaag gcaggtgagc
     6001 agcaggaaac agcctcccgg gagttagtca aggagcctgc gagggcagga gacagacagc
     6061 ccgagtggct ggaagagcaa cagggacgcc agttctgcag cacacaggca gcgctgcagg
     6121 ctatggagcg ggaggcagag cagatgggca atgagctgga acggctgcgg gccgcgctga
     6181 tggagagcca ggggcagcag caggaggagc gtgggcagca ggaaagggag gtggcgcggc
     6241 tgacccagga gcggggccgt gcccaggctg accttgccct ggagaaggcg gccagagcag
     6301 agcttgagat gcggctgcag aacgccctca acgagcagcg tgtggagttc gctaccctgc
     6361 aagaggcact ggctcatgcc ctgacggaaa aggaaggcaa ggaccaggag ttggccaagc
     6421 ttcgtggtct ggaggcagcc cagataaaag agctggagga acttcggcaa accgtgaagc
     6481 aactgaagga acagctggct aagaaagaaa aggagcacgc atctggctca ggagcccaat
     6541 ctgaggctgc tggcaggaca gagccaacag gccccaagct ggaagcactg cgggcagagg
     6601 tgagcaagct ggaacagcaa tgccagaagc agcaggagca ggctgacagc ctggaacgca
     6661 gcctcgaggc tgagcgggcc tcccgggctg agcgggacag tgctctggag actctgcagg
     6721 gccagttaga ggagaaggcc caggagctag ggcacagtca gagtgcctta gcctcggccc
     6781 aacgggagtt ggctgccttc cgcaccaagg tacaagacca cagcaaggct gaagatgagt
     6841 ggaaggccca ggtggcccgg ggccggcaag aggctgagag gaaaaatagc ctcatcagca
     6901 gcttggagga ggaggtgtcc atcctgaatc gccaggtcct ggagaaggag ggggagagca
     6961 aggagttgaa gcggctggtg atggccgagt cagagaagag ccagaagctg gaggagaggc
     7021 tgcgcctgct gcaggcagag acagccagca acagtgccag agctgcagaa cgcagctctg
     7081 ctctgcggga ggaggtgcag agcctccggg aggaggctga gaaacagcgg gtggcttcag
     7141 agaacctgcg gcaggagctg acctcacagg ctgagcgtgc ggaggagctg ggccaagaat
     7201 tgaaggcgtg gcaggagaag ttcttccaga aagagcaggc cctctccacc ctgcagctcg
     7261 agcacaccag cacacaggcc ctggtgagtg agctgctgcc agctaagcac ctctgccagc
     7321 agctgcaggc cgagcaggcc gctgccgaga aacgccaccg tgaggagctg gagcagagca
     7381 agcaggccgc tgggggactg cgggcagagc tgctgcgggc ccagcgggag cttggggagc
     7441 tgattcctct gcggcagaag gtggcagagc aggagcgaac agctcagcag ctgcgggcag
     7501 agaaggccag ctatgcagag cagctgagca tgctgaagaa ggcgcatggc ctgctggcag
     7561 aggagaaccg ggggctgggt gagcgggcca accttggccg gcagtttctg gaagtggagt
     7621 tggaccaggc ccgggaaaag tatgtccaag agttggcagc cgtacgtgct gatgctgaga
     7681 cccgtctggc tgaggtgcag cgagaagcac agagcactgc ccgggagctg gaggtgatga
     7741 ctgccaagta tgagggtgcc aaggtcaagg tcctggagga gaggcagcgg ttccaggaag
     7801 agaggcagaa actcactgcc caggtggaag aactgnagka agagaggcag aaactcactg
     7861 cccaggtgga agaactgagt aagaaactgg ctgactctga ccaagccagc aaggtgcagc
     7921 agcagaagct gaaggctgtc caggctcagg gaggcgagag ccagcaggag gcccagcgct
     7981 tccaggccca gctgaatgaa ctgcaagccc agttgagcca gaaggagcag gcagctgagc
     8041 actataagct gcagatggag aaagccaaaa cacattatga tgccaagaag cagcagaacc
     8101 aagagctgca ggagcagctg cggagcctgg agcagctgca gaaggaaaac aaagagctgc
     8161 gagctgaagc tgaacggctg ggccatgagc tacagcaggc tgggctgaag accaaggagg
     8221 ctgaacagac ctgccgccac cttactgccc aggtgcgcag cctggaggca caggttgccc
     8281 atgcagacca gcagcttcga gacctgggca aattccaggt ggcaactgat gctttaaaga
     8341 gccgtgagcc ccaggctaag ccccagctgg acttgagtat tgacagcctg gatctgagct
     8401 gcgaggaggg gaccccactc agtatcacca gcaagctgcc tcgtacccag ccagacggca
     8461 ccagcgtccc tggagaacca gcctcaccta tctcccagcg cctgcccccc aaggtagaat
     8521 ccctggagag tctctacttc actcccatcc ctgctcggag tcaggccccc ctggagagca
     8581 gcctggactc cctgggagac gtcttcctgg actcgggtcg taagacccgc tccgctcgtc
     8641 ggcgcaccac gcagatcatc aacatcacca tgaccaagaa gctagatgtg gaagagccag
     8701 acagcgccaa ctcatcgttc tacagcacgc ggtctgctcc tgcttcccag gctagcctgc
     8761 gagccacctc ctctactcag tctctagctc gcctgggttc tcccgattat ggcaactcag
     8821 ccctgctcag cttgcctggc taccgcccca ccactcgcag ttctgctcgt cgttcccagg
     8881 ccggggtgtc cagtggggcc cctccaggaa ggaacagctt ctacatgggc acttgccagg
     8941 atgagcctga gcagctggat gactggaacc gcattgcaga gctgcagcag cgcaatcgag
     9001 tgtgcccccc acatctgaag acctgctatc ccctggagtc caggccttcc ctgagcctgg
     9061 gcaccatcac agatgaggag atgaaaactg gagaccccca agagaccctg cgccgagcca
     9121 gcatgcagcc aatccagata gccgagggca ctggcatcac cacccgggtc tccctagagc
     9181 cccaccaggg ccctggaact cctgagtcta agaaggccac cagctgtttc ccacgcccca
     9241 tgactccccg agaccgacat gaagggcgca aacagagcac tactgaggcc cagaagaaag
     9301 cagctccagc ttctactaaa caggctgacc ggcgccagtc gatggccttc agcatcctca
     9361 acacacccaa gaagctaggg aacagccttc tgcggcgggg agcctcaaag aaggccctgt
     9421 ccaaggcttc ccccaacacc cgcagtggaa cccgccgttc tccgcgcatt gccaccacca
     9481 cagccagtgc cgccactgct gccgccattg gtgccacccc tcgagccaag ggcaaggcaa
     9541 agcacggagg tggatctacg cgtggaggtg gatctgccac catggtgtct aagggcgaag
     9601 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg aacaaccacc
     9661 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     9721 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct accagcttca
     9781 tgtacggcag cagaaccttc atcaaccaca cccagggcat ccccgacttc tttaagcagt
     9841 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     9901 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     9961 gggtgaactt cccatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcca
    10021 acaccgagat gctgtacccc gctgacggcg gcctggaagg cagaaccgac atggccctga
    10081 agctcgtggg cgggggccac ctgatctgca acttcaagac cacatacaga tccaagaaac
    10141 ccgctaagaa cctcaagatg cccggcgtct actatgtgga ccacagactg gaaagaatca
    10201 aggaggccga caaagagacc tacgtcgagc agcacgaggt ggctgtggcc agatactgcg
    10261 acctccctag caaactgggg cacaaactta atggcatgga cgagctgtac aagtccggac
    10321 tcagatctcg agctcaagct tcgaacgaat tcggcattga tctgagcggc ctgaccctgc
    10381 aggaattcag ctccccgaaa cgccctaagc tgctgcgtga atattacgat tggctggttg
    10441 ataacagctt taccccatat ctggtggtgg atgccacata cctgggcgtg aacgtgcccg
    10501 tggagtatgt gaaagacggt cagatcgtgc tgaatctgtc tgcaagtgcg accggcaacc
    10561 tgcaactgac aaatgatttt atccagttca acgcccgctt taagggcgtg tctcgtgaac
    10621 tgtatatccc gatgggtgcc gctctggcca tttacgctcg cgagaacggc gatggtgtga
    10681 tgttcgaacc agaagaaatc tatgacgagc tgaatattgg ttaaaccggt ggatccagat
    10741 ctctgcagca tatgtctaga ggatcataat cagccatacc acatttgtag aggttttact
    10801 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    10861 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    10921 tttcacaaat aaagcatttt tttcactgcc gacgtcaggt ggcacttttc ggggttgggg
    10981 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
    11041 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
    11101 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
    11161 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
    11221 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
    11281 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
    11341 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
    11401 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
    11461 atcaccgacc tctctcccca gggggatcca ccggagctta ccatggatag atccggaaag
    11521 cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga cagcgtctcc
    11581 gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga tgtaggaggg
    11641 cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga tcgttatgtt
    11701 tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat tggggaattc
    11761 agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt gcaagacctg
    11821 cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga tgcgatcgct
    11881 gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg aatcggtcaa
    11941 tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta tcactggcaa
    12001 actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga gctgatgctt
    12061 tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg ctccaacaat
    12121 gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc gatgttcggg
    12181 gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc ttgtatggag
    12241 cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc gcggctccgg
    12301 gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga cggcaatttc
    12361 gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg agccgggact
    12421 gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg ctgtgtagaa
    12481 gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa ggaatagagt
    12541 agatgccgac cgaacaagag ctgatttcga gaacgcctca gccagcaact cgcgcgagcc
    12601 tagcaaggca aatgcgagag aacggcctta cgcttggtgg cacagttctc gtccacagtt
    12661 cgctaagctc gctcggctgg gtcgcgggag ggccggtcgc agtgattcag gcccttctgg
    12721 attgtgttgg tccccagggc acgattgtca tgcccacgca ctcgggtgat ctgactgatc
    12781 ccgcagattg gagatcgccg cccgtgcctg ccgattgggt gcagatctct agagctcgct
    12841 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
    12901 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
    12961 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
    13021 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
    13081 ctgaggcgga aagaaccagc tggggctcga tgagggagct gcggtggagg aggtggagag
    13141 aaggccgcac ccttctgggc agggggaggg gagtgccgca atacctttat gggagttctc
    13201 tgctgcctcc cgtcttgtaa ggaccgccct gggcctggaa gaagccctcc ctcctttcct
    13261 cctcgcgtga tctcgtcatc gcctccatgt cgagtcgctt ctcgattatg ggcgggattc
    13321 ttttgcctag gcttaagggg ctaacttggt ccctgggcgt tgccctgcag gggagtgagc
    13381 agctgtaaga tttgaggggc gactccgatt agtttatctt cccacggact agagttggtg
    13441 tcgaggttat tgtaataagg gtggggtagg gaaatggagc ttagtcattc acctggggct
    13501 gattttatgc aacgagactg cggattatca ctaatcattt ttggagcatt tttctagaga
    13561 cagacataaa gcatgatcac ctgagtttta taccatttga gacccttgct gcaccaccaa
    13621 agtgtagcat caggttaaat cttaatagaa aaattttagc ttttgcttga gaaaccagtg
    13681 cttccctccc tcaccctctc tccccaggct ctctacccct ttgcatccct accaggcatc
    13741 ttagcaactc tcactcatac ttgatcccat tttccatttg ttgtacttgc tcctccagta
    13801 ttcagacata gcactagctt tctccctctc ttgatcttgg gtagcctggt gtctcgcgaa
    13861 accagacaga ttggttccac cacaaattaa ggcttgagct ggggcttgac tcttacccag
    13921 cagtgctttt attcctccct agttcacgtt cttaaatgtt tatcttgatt ttcattttat
    13981 cctttttcct tagctgggat tctgtccctg accgtcttca cagtccaggt gatcttgact
    14041 actgctttac agagaattgg atctgaggtt aggcaacatc tccctttttc ttcctctaaa
    14101 tacctctcat ttctgttctt accagttagt aactgatctc agatgcctgt gtgatagctg
    14161 gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca
    14221 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccggg
    14281 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
    14341 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
    14401 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
    14461 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
    14521 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
    14581 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
    14641 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
    14701 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
    14761 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
    14821 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
    14881 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
    14941 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
    15001 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
    15061 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
    15121 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
    15181 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
    15241 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
    15301 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
    15361 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
    15421 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
    15481 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
    15541 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
    15601 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
    15661 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
    15721 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
    15781 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
    15841 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
    15901 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
    15961 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
    16021 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
    16081 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
    16141 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
    16201 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
    16261 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
    16321 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
