LOCUS       sequence-225128-        8323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  single guide RNA targeting PSMB5; guide 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, 
            Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed 
            A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, 
            Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare 
            B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, 
            Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, 
            Beroukhim R, Ligon KL, Boehm JS, Hahn WC
  TITLE     Renal medullary carcinomas depend upon SMARCB1 loss and are 
            sensitive to proteasome inhibition.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161.
  PUBMED    30860482
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 44161. doi"; pages: " 
            10.7554/eLife.44161"
COMMENT     Sequence Label: pXPR_BRD003 PSMB5 guide 2
FEATURES             Location/Qualifiers
     source          1..8323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             31..75
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        253..501
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     253..273
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     424..443
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        523..598
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    654..771
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        910..2088
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1141..2079
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2036..2056
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             2101..2700
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2101..2120)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2597..2617
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2716..3304
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2769..2789)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3187..3198)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3376..3609
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3687..3808
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3724..3743)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3778..3797
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3848..3983
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4003..4022)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4004..4022)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4032..4049)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4032..4048)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4041..4063)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4190..4645
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4277..4296)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4487..4508
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4671..4775
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4776..4844,4845..5636)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4994..5013)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5807..6395
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6296..6315
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6549..6566
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6683..6704
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6719..6724,6725..6742,6743..6749)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6757..6773
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6762..6784
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6781..6797
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6781..6797
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6816..6836
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6818..6836
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6864..7090
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7091..7271
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7318..7443
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7936..8169
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
       61 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
      121 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
      181 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
      241 aggggaccca gagagggcct atttcccatg attccttcat atttgcatat acgatacaag
      301 gctgttagag agataattag aattaatttg actgtaaaca caaagatatt agtacaaaat
      361 acgtgacgta gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa
      421 atggactatc atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc
      481 ttgtggaaag gacgaaacac cgtgaaggga accggatttc aggttttaga gctagaaata
      541 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt
      601 ttttaagctt ggcgtaacta gatcttgaga caaatggcag tattcatcca caattttaaa
      661 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
      721 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
      781 tacagggaca gcagagatcc actttggcgc cggctcgagg gggcccgggt gcaaagatgg
      841 ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt cttgaaagga
      901 gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
      961 aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg cggggtaaac
     1021 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     1081 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     1141 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     1201 ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct tcgggttgga
     1261 agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg tgcttgagtt
     1321 gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct tcgcgcctgt
     1381 ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc tgcgacgctt
     1441 tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg tatttcggtt
     1501 tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc ggcgaggcgg
     1561 ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg ccggcctgct
     1621 ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag gctggcccgg
     1681 tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc agggagctca
     1741 aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca aaggaaaagg
     1801 gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc gccgtccagg
     1861 cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt
     1921 tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac
     1981 ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag
     2041 cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgacg tacggccacc
     2101 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     2161 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     2221 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     2281 atcggcaagg tgtgggtcgc ggacgacggc gccgccgtgg cggtctggac cacgccggag
     2341 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     2401 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     2461 cccgcgtggt tcctggccac cgtcggagtc tcgcccgacc accagggcaa gggtctgggc
     2521 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     2581 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     2641 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     2701 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2761 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2821 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2881 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2941 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3001 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3061 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     3121 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3181 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3241 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3301 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     3361 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     3421 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3481 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3541 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3601 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     3661 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     3721 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     3781 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     3841 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     3901 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     3961 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     4021 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     4081 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     4141 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     4201 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     4261 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     4321 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     4381 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     4441 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     4501 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     4561 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     4621 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     4681 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     4741 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     4801 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     4861 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     4921 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     4981 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     5041 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     5101 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     5161 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     5221 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     5281 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     5341 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     5401 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     5461 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     5521 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     5581 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     5641 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     5701 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     5761 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     5821 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     5881 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     5941 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     6001 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     6061 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     6121 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     6181 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     6241 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     6301 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     6361 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     6421 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     6481 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     6541 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     6601 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     6661 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     6721 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     6781 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     6841 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     6901 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     6961 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     7021 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     7081 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7141 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7201 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7261 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     7321 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     7381 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     7441 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     7501 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     7561 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     7621 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     7681 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     7741 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     7801 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     7861 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     7921 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     7981 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     8041 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     8101 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     8161 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     8221 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     8281 gtgggacaga gaaattaaca attacacaag cttaatacac tcc
//
