LOCUS       sequence-225131-       15137 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NuMA(1-1700)-RFP-Nano from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15137)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 15137)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK652
FEATURES             Location/Qualifiers
     source          1..15137
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1172..1253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1370..2116)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2135..2645)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2665..2713
                     /note="synthetic polyadenylation signal"
     misc_feature    2727..2818
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2767..2786
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2822..3189
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2830..2848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2866..2884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2902..2920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3046..3064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      8347..8356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8353..9084
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             9160..9489
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    9639..9720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        9742..10252
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             10289..11308
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             10289..11308
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(11618..11635)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11624..11848
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12949..12969)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12949..12967)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12988..13004)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12988..13004)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13001..13023)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13012..13028
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13036..13042,13043..13060,13061..13066))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13081..13102
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(13390..13978)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13470..13489)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14149..14940,14941..15009))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14772..14791
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(15010..15114)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gaagctgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt atttgtgaaa
     1201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1261 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1321 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagatt tacccgggga
     1381 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
     1441 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
     1501 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
     1561 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
     1621 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
     1681 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
     1741 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
     1801 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
     1861 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
     1921 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
     1981 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
     2041 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
     2101 tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt
     2161 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2221 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2281 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2341 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2401 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2461 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     2521 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     2581 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     2641 accccgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     2701 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     2761 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     2821 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2881 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2941 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3001 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3061 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     3121 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     3181 cctcgtaaag aattcgcggc cgcacgcgtg ccaccatgac actccacgcc acccgggggg
     3241 ctgcactcct ctcttgggtg aacagtctac acgtggctga ccctgtggag gctgtgctgc
     3301 agctccagga ctgcagcatc ttcatcaaga tcattgacag aatccatggc actgaagagg
     3361 gacagcaaat cttgaagcag ccggtgtcag agagactgga ctttgtgtgc agttttctgc
     3421 agaaaaatcg aaaacatccc tcttccccag aatgcctggt atctgcacag aaggtgctag
     3481 agggatcaga gctggaactg gcgaagatga ccatgctgct cttataccac tctaccatga
     3541 gctccaaaag tcccagggac tgggaacagt ttgaatataa aattcaggct gagttggctg
     3601 tcattcttaa atttgtgctg gaccatgagg acgggctaaa ccttaatgag gacctagaga
     3661 acttcctaca gaaagctcct gtgccttcta cctgttctag cacattccct gaagagctct
     3721 ccccacctag ccaccaggcc aagagggaga ttcgcttcct agagctacag aaggttgcct
     3781 cctcttccag tgggaacaac tttctctcag gttctccagc ttctcccatg ggtgatatcc
     3841 tgcagacccc acagttccag atgagacggc tgaagaagca gcttgctgat gagagaagta
     3901 atagggatga gctggagctg gagctagctg agaaccgcaa gctcctcacc gagaaggatg
     3961 cacagatagc catgatgcag cagcgcattg accgcctagc cctgctgaat gagaagcagg
     4021 cggccagccc actggagccc aaggagcttg aggagctgcg tgacaagaat gagagcctta
     4081 ccatgcggct gcatgaaacc ctgaagcagt gccaggacct gaagacagag aagagccaga
     4141 tggatcgcaa aatcaaccag ctttcggagg agaatggaga cctttccttt aagctgcggg
     4201 agtttgccag tcatctgcag cagctacagg atgccctcaa tgagctgacg gaggagcaca
     4261 gcaaggccac tcaggagtgg ctagagaagc aggcccagct ggagaaggag ctcagcgcag
     4321 ccctgcagga caagaaatgc cttgaagaga agaacgaaat ccttcaggga aaactttcac
     4381 agctggaaga acacttgtcc cagctgcagg ataacccacc ccaggagaag ggcgaggtgc
     4441 tgggtgatgt cttgcagctg gaaaccttga agcaagaggc agccactctt gctgcaaaca
     4501 acacacagct ccaagccagg gtagagatgc tggagactga gcgaggccag caggaagcca
     4561 agctgcttgc tgagcggggc cacttcgaag aagaaaagca gcagctgtct agcctgatca
     4621 ctgacctgca gagctccatc tccaacctca gccaggccaa ggaagagctg gagcaggcct
     4681 cccaggctca tggggcccgg ttgactgccc aggtggcctc tctgacctct gagctcacca
     4741 cactcaatgc caccatccag caacaggatc aagaactggc tggcctgaag cagcaggcca
     4801 aagagaagca ggcccagcta gcacagaccc tccaacagca agaacaggcc tcccagggcc
     4861 tccgccacca ggtggagcag ctaagcagta gcctgaagca gaaggagcag cagttgaagg
     4921 aggtagcgga gaagcaggag gcaactaggc aggaccatgc ccagcaactg gccactgctg
     4981 cagaggagcg agaggcctcc ttaagggagc gggatgcggc tctcaagcag ctggaggcac
     5041 tggagaagga gaaggctgcc aagctggaga ttctgcagca gcaacttcag gtggctaatg
     5101 aagcccggga cagtgcccag acctcagtga cacaggccca gcgggagaag gcagagctga
     5161 gccggaaggt ggaggaactc caggcctgtg ttgagacagc ccgccaggaa cagcatgagg
     5221 cccaggccca ggttgcagag ctagagttgc agctgcggtc tgagcagcaa aaagcaactg
     5281 agaaagaaag ggtggcccag gagaaggacc agctccagga gcagctccag gccctcaaag
     5341 agtccttgaa ggtcaccaag ggcagccttg aagaggagaa gcgcagggct gcagatgccc
     5401 tggaagagca gcagcgttgt atctctgagc tgaaggcaga gacccgaagc ctggtggagc
     5461 agcataagcg ggaacgaaag gagctggaag aagagagggc tgggcgcaag gggctggagg
     5521 ctcgattaca gcagcttggg gaggcccatc aggctgagac tgaagtcctg cggcgggagc
     5581 tggcagaggc catggctgcc cagcacacag ctgagagtga gtgtgagcag ctcgtcaaag
     5641 aagtagctgc ctggcgtgag cggtatgagg atagccagca agaggaggca cagtatggcg
     5701 ccatgttcca ggaacagctg atgactttga aggaggaatg tgagaaggcc cgccaggagc
     5761 tgcaggaggc aaaggagaag gtggcaggca tagaatccca cagcgagctc cagataagcc
     5821 ggcagcagaa caaactagct gagctccatg ccaacctggc cagagcactc cagcaggtcc
     5881 aagagaagga agtcagggcc cagaagcttg cagatgacct ctccactctg caggaaaaga
     5941 tggctgccac cagcaaagag gtggcccgct tggagacctt ggtgcgcaag gcaggtgagc
     6001 agcaggaaac agcctcccgg gagttagtca aggagcctgc gagggcagga gacagacagc
     6061 ccgagtggct ggaagagcaa cagggacgcc agttctgcag cacacaggca gcgctgcagg
     6121 ctatggagcg ggaggcagag cagatgggca atgagctgga acggctgcgg gccgcgctga
     6181 tggagagcca ggggcagcag caggaggagc gtgggcagca ggaaagggag gtggcgcggc
     6241 tgacccagga gcggggccgt gcccaggctg accttgccct ggagaaggcg gccagagcag
     6301 agcttgagat gcggctgcag aacgccctca acgagcagcg tgtggagttc gctaccctgc
     6361 aagaggcact ggctcatgcc ctgacggaaa aggaaggcaa ggaccaggag ttggccaagc
     6421 ttcgtggtct ggaggcagcc cagataaaag agctggagga acttcggcaa accgtgaagc
     6481 aactgaagga acagctggct aagaaagaaa aggagcacgc atctggctca ggagcccaat
     6541 ctgaggctgc tggcaggaca gagccaacag gccccaagct ggaagcactg cgggcagagg
     6601 tgagcaagct ggaacagcaa tgccagaagc agcaggagca ggctgacagc ctggaacgca
     6661 gcctcgaggc tgagcgggcc tcccgggctg agcgggacag tgctctggag actctgcagg
     6721 gccagttaga ggagaaggcc caggagctag ggcacagtca gagtgcctta gcctcggccc
     6781 aacgggagtt ggctgccttc cgcaccaagg tacaagacca cagcaaggct gaagatgagt
     6841 ggaaggccca ggtggcccgg ggccggcaag aggctgagag gaaaaatagc ctcatcagca
     6901 gcttggagga ggaggtgtcc atcctgaatc gccaggtcct ggagaaggag ggggagagca
     6961 aggagttgaa gcggctggtg atggccgagt cagagaagag ccagaagctg gaggagaggc
     7021 tgcgcctgct gcaggcagag acagccagca acagtgccag agctgcagaa cgcagctctg
     7081 ctctgcggga ggaggtgcag agcctccggg aggaggctga gaaacagcgg gtggcttcag
     7141 agaacctgcg gcaggagctg acctcacagg ctgagcgtgc ggaggagctg ggccaagaat
     7201 tgaaggcgtg gcaggagaag ttcttccaga aagagcaggc cctctccacc ctgcagctcg
     7261 agcacaccag cacacaggcc ctggtgagtg agctgctgcc agctaagcac ctctgccagc
     7321 agctgcaggc cgagcaggcc gctgccgaga aacgccaccg tgaggagctg gagcagagca
     7381 agcaggccgc tgggggactg cgggcagagc tgctgcgggc ccagcgggag cttggggagc
     7441 tgattcctct gcggcagaag gtggcagagc aggagcgaac agctcagcag ctgcgggcag
     7501 agaaggccag ctatgcagag cagctgagca tgctgaagaa ggcgcatggc ctgctggcag
     7561 aggagaaccg ggggctgggt gagcgggcca accttggccg gcagtttctg gaagtggagt
     7621 tggaccaggc ccgggaaaag tatgtccaag agttggcagc cgtacgtgct gatgctgaga
     7681 cccgtctggc tgaggtgcag cgagaagcac agagcactgc ccgggagctg gaggtgatga
     7741 ctgccaagta tgagggtgcc aaggtcaagg tcctggagga gaggcagcgg ttccaggaag
     7801 agaggcagaa actcactgcc caggtggaag aactgagtaa gaaactggcw gamwctcact
     7861 gcccaggtgg aagaactgag taagaaactg gctgactctg accaagccag caaggtgcag
     7921 cagcagaagc tgaaggctgt ccaggctcag ggaggcgaga gccagcagga ggcccagcgc
     7981 ttccaggccc agctgaatga actgcaagcc cagttgagcc agaaggagca ggcagctgag
     8041 cactataagc tgcagatgga gaaagccaaa acacattatg atgccaagaa gcagcagaac
     8101 caagagctgc aggagcagct gcggagcctg gagcagctgc agaaggaaaa caaagagctg
     8161 cgagctgaag ctgaacggct gggccatgag ctacagcagg ctgggctgaa gaccaaggag
     8221 gctgaacaga cctgccgcca ccttactgcc caggtgcgca gcctggaggc acaggttgcc
     8281 catgcagacc agcagcttcg agacctgggc aaattcggag gtggatctac gcgtggaggt
     8341 ggatctgcca ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag
     8401 ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc
     8461 aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc
     8521 ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac
     8581 acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga
     8641 gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac
     8701 ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg
     8761 atgcagaaga aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc
     8821 ggcctggaag gcagaaccga catggccctg aagctcgtgg gcgggggcca cctgatctgc
     8881 aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc
     8941 tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag
     9001 cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt
     9061 aatggcatgg acgagctgta caagtccgga ctcagatctc gagctcaagc ttcgaacgaa
     9121 ttcggcattg atctgagcgg cctgaccctg caggaattca gctccccgaa acgccctaag
     9181 ctgctgcgtg aatattacga ttggctggtt gataacagct ttaccccata tctggtggtg
     9241 gatgccacat acctgggcgt gaacgtgccc gtggagtatg tgaaagacgg tcagatcgtg
     9301 ctgaatctgt ctgcaagtgc gaccggcaac ctgcaactga caaatgattt tatccagttc
     9361 aacgcccgct ttaagggcgt gtctcgtgaa ctgtatatcc cgatgggtgc cgctctggcc
     9421 atttacgctc gcgagaacgg cgatggtgtg atgttcgaac cagaagaaat ctatgacgag
     9481 ctgaatattg gttaaaccgg tggatccaga tctctgcagc atatgtctag aggatcataa
     9541 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     9601 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     9661 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9721 cgacgtcagg tggcactttt cggggttggg gttgcgcctt ttccaaggca gccctgggtt
     9781 tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa cgcagcggcg ccgaccctgg
     9841 gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg atcttcgccg ctacccttgt
     9901 gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg gaaggttcct tgcggttcgc
     9961 ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact agtaccctcg cagacggaca
    10021 gcgccaggga gcaatggcag cgcgccgacc gcgatgggct gtggccaata gcggctgctc
    10081 agcagggcgc gccgagagca gcggccggga aggggcggtg cgggaggcgg ggtgtggggc
    10141 ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca ttctgcaagc ctccggagcg
    10201 cacgtcggca gtcggctccc tcgttgaccg aatcaccgac ctctctcccc agggggatcc
    10261 accggagctt accatggata gatccggaaa gcctgaactc accgcgacgt ctgtcgagaa
    10321 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
    10381 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
    10441 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
    10501 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
    10561 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
    10621 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
    10681 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
    10741 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
    10801 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
    10861 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
    10921 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
    10981 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
    11041 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
    11101 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
    11161 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
    11221 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
    11281 cagcactcgt ccgagggcaa aggaatagag tagatgccga ccgaacaaga gctgatttcg
    11341 agaacgcctc agccagcaac tcgcgcgagc ctagcaaggc aaatgcgaga gaacggcctt
    11401 acgcttggtg gcacagttct cgtccacagt tcgctaagct cgctcggctg ggtcgcggga
    11461 gggccggtcg cagtgattca ggcccttctg gattgtgttg gtccccaggg cacgattgtc
    11521 atgcccacgc actcgggtga tctgactgat cccgcagatt ggagatcgcc gcccgtgcct
    11581 gccgattggg tgcagatctc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
    11641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
    11701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
    11761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
    11821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctcg
    11881 atgagggagc tgcggtggag gaggtggaga gaaggccgca cccttctggg cagggggagg
    11941 ggagtgccgc aataccttta tgggagttct ctgctgcctc ccgtcttgta aggaccgccc
    12001 tgggcctgga agaagccctc cctcctttcc tcctcgcgtg atctcgtcat cgcctccatg
    12061 tcgagtcgct tctcgattat gggcgggatt cttttgccta ggcttaaggg gctaacttgg
    12121 tccctgggcg ttgccctgca ggggagtgag cagctgtaag atttgagggg cgactccgat
    12181 tagtttatct tcccacggac tagagttggt gtcgaggtta ttgtaataag ggtggggtag
    12241 ggaaatggag cttagtcatt cacctggggc tgattttatg caacgagact gcggattatc
    12301 actaatcatt tttggagcat ttttctagag acagacataa agcatgatca cctgagtttt
    12361 ataccatttg agacccttgc tgcaccacca aagtgtagca tcaggttaaa tcttaataga
    12421 aaaattttag cttttgcttg agaaaccagt gcttccctcc ctcaccctct ctccccaggc
    12481 tctctacccc tttgcatccc taccaggcat cttagcaact ctcactcata cttgatccca
    12541 ttttccattt gttgtacttg ctcctccagt attcagacat agcactagct ttctccctct
    12601 cttgatcttg ggtagcctgg tgtctcgcga aaccagacag attggttcca ccacaaatta
    12661 aggcttgagc tggggcttga ctcttaccca gcagtgcttt tattcctccc tagttcacgt
    12721 tcttaaatgt ttatcttgat tttcatttta tcctttttcc ttagctggga ttctgtccct
    12781 gaccgtcttc acagtccagg tgatcttgac tactgcttta cagagaattg gatctgaggt
    12841 taggcaacat ctcccttttt cttcctctaa atacctctca tttctgttct taccagttag
    12901 taactgatct cagatgcctg tgtgatagct ggtacccagc ttttgttccc tttagtgagg
    12961 gttaattgcg cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
    13021 gctcacaatt ccacacaaca tacgagccgg gagcataaag tgtaaagcct ggggtgccta
    13081 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
    13141 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
    13201 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    13261 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    13321 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    13381 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    13441 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    13501 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    13561 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    13621 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    13681 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    13741 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    13801 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
    13861 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    13921 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    13981 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    14041 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
    14101 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
    14161 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
    14221 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
    14281 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
    14341 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
    14401 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
    14461 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
    14521 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
    14581 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
    14641 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
    14701 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
    14761 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
    14821 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
    14881 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
    14941 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
    15001 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
    15061 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
    15121 tccccgaaaa gtgccac
//
