LOCUS       sequence-225132-        8830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Inducible vector (untagged) with SMARCB1 variant 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8830)
  AUTHORS   Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, 
            Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed 
            A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, 
            Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare 
            B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, 
            Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, 
            Beroukhim R, Ligon KL, Boehm JS, Hahn WC
  TITLE     Renal medullary carcinomas depend upon SMARCB1 loss and are 
            sensitive to proteasome inhibition.
  JOURNAL   Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161.
  PUBMED    30860482
REFERENCE   2  (bases 1 to 8830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2019-03-12"; volume: "8. pii: 44161. doi"; pages: " 
            10.7554/eLife.44161"
COMMENT     Sequence Label: pLXI_TRC401 SMARCB1 var 2
FEATURES             Location/Qualifiers
     source          1..8830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        40..354
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    48..66
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    84..102
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    119..137
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    155..173
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    191..209
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    226..244
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    262..280
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     327..351
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    375..399
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1531..1555)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1573..1590
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        1600..2110
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2029..2047
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2120..2716
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2120..2139)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2616..2636
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2717..2770
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(2771..3388,3389..3394,3395..3430,3431..3433,3434..3469,
                     3470..3472,3473..3508,3509..3517)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(3557..3577)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3975..3986)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4191..4424
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4496..4617
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4533..4552)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4587..4606
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4657..4792
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4792..4863
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4864..4932,4933..5724)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5082..5101)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5895..6483
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6384..6403
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6637..6654
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6771..6792
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6807..6812,6813..6830,6831..6837)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6845..6861
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6850..6872
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6869..6885
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6869..6885
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6904..6924
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6906..6924
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6952..7178
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7179..7359
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7406..7531
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8024..8257
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8442..8486
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8699..8816
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggacagcaga gatccacttt ggccgcgaat cgatatgtcg agtttactcc ctatcagtga
       61 tagagaacgt atgtcgagtt tactccctat cagtgataga gaacgatgtc gagtttactc
      121 cctatcagtg atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgtatg
      181 tcgagtttac tccctatcag tgatagagaa cgtatgtcga gtttatccct atcagtgata
      241 gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg aggtaggcgt
      301 gtacggtggg aggcctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
      361 attggctagc atcaacaagt ttgtacaaaa aagttggcac catgatgatg atggcgctga
      421 gcaagacctt cgggcagaag cccgtgaagt tccagctgga ggacgacggc gagttctaca
      481 tgatcggctc cgaggtggga aactacctcc gtatgttccg aggttctctg tacaagagat
      541 acccctcact ctggaggcga ctagccactg tggaagagag gaagaaaata gttgcatcgt
      601 cacatgatca cggatacacg actctagcca ccagtgtgac cctgttaaaa gcctcggaag
      661 tggaagagat tctggatggc aacgatgaga agtacaaggc tgtgtccatc agcacagagc
      721 cccccaccta cctcagggaa cagaaggcca agaggaacag ccagtgggta cccaccctgc
      781 ccaacagctc ccaccactta gatgccgtgc catgctccac aaccatcaac aggaaccgca
      841 tgggccgaga caagaagaga accttccccc tttgctttga tgaccatgac ccagctgtga
      901 tccatgagaa cgcatctcag cccgaggtgc tggtccccat ccggctggac atggagatcg
      961 atgggcagaa gctgcgagac gccttcacct ggaacatgaa tgagaagttg atgacgcctg
     1021 agatgttttc agaaatcctc tgtgacgatc tggatttgaa cccgctgacg tttgtgccag
     1081 ccatcgcctc tgccatcaga cagcagatcg agtcctaccc cacggacagc atcctggagg
     1141 accagtcaga ccagcgcgtc atcatcaagc tgaacatcca tgtgggaaac atttccctgg
     1201 tggaccagtt tgagtgggac atgtcagaga aggagaactc accagagaag tttgccctga
     1261 agctgtgctc ggagctgggg ttgggcgggg agtttgtcac caccatcgca tacagcatcc
     1321 ggggacagct gagctggcat cagaagacct acgccttcag cgagaaccct ctgcccacag
     1381 tggagattgc catccggaac acgggcgatg cggaccagtg gtgcccactg ctggagactc
     1441 tgacagacgc tgagatggag aagaagatcc gcgaccagga caggaacacg aggcggatga
     1501 ggcgtcttgc caacacggcc ccggcctggt tgccaacttt cttgtacaaa gtggtttagt
     1561 aatgaaccgg tccaccacca ccaccaccac taaggatccg gggttggggt tgcgcctttt
     1621 ccaaggcagc cctgggtttg cgcagggacg cggctgctct gggcgtggtt ccgggaaacg
     1681 cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat
     1741 cttcgccgct acccttgtgg gccccccggc gacgcttcct gctccgcccc taagtcggga
     1801 aggttccttg cggttcgcgg cgtgccggac gtgacaaacg gaagccgcac gtctcactag
     1861 taccctcgca gacggacagc gccagggagc aatggcagcg cgccgaccgc gatgggctgt
     1921 ggccaatagc ggctgctcag cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg
     1981 ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt
     2041 ctgcaagcct ccggagcgca cgtcggcagt cggctccctc gttgaccgaa tcaccgacct
     2101 ctctccccag caattcacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     2161 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     2221 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     2281 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     2341 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     2401 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     2461 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     2521 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     2581 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     2641 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     2701 ccgcaagccc ggtgccgaag gtagaggttc tctcctcact tgtggtgatg ttgaagaaaa
     2761 ccctggtcca atgtctagac tggacaagag caaagtcata aacggagctc tggaattact
     2821 caatggtgtc ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga
     2881 gcagcctacc ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat
     2941 cgagatgctg gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga
     3001 ctttctgcgg aacaacgcca agtcataccg ctgtgctctc ctctcacatc gcgacggggc
     3061 taaagtgcat ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct
     3121 cgcgttcctg tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt
     3181 gggccacttt acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga
     3241 aagagagaca cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt
     3301 cgaccggcag ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct
     3361 ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac gcccttgacg attttgactt
     3421 agacatgctc ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc
     3481 tcttgacgat tttgaccttg acatgctccc cgggtaacta agtaaggatc gatccaagat
     3541 atcgtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     3601 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     3661 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     3721 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     3781 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     3841 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     3901 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     3961 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     4021 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     4081 ccgcctcccc gcctgtttcg cctcggcgtc cggactagag gtacctttaa gaccaatgac
     4141 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     4201 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     4261 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     4321 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     4381 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     4441 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     4501 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     4561 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     4621 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     4681 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     4741 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctttcaaat
     4801 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     4861 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     4921 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     4981 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5041 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5101 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5161 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5221 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5281 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5341 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5401 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5461 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5521 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5581 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5641 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5701 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     5761 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     5821 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     5881 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     5941 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6001 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6061 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6121 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6181 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6241 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6301 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6361 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6421 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6481 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6541 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6601 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6661 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6721 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     6781 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     6841 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
     6901 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt
     6961 atgcaatact cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag
     7021 gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat
     7081 taggaaggca acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca
     7141 gagatattgt atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga
     7201 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     7261 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     7321 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt
     7381 gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg
     7441 cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg
     7501 ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg
     7561 atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt
     7621 atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga
     7681 aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat cagaagaact
     7741 tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa
     7801 agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc
     7861 acagcaagcg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     7921 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     7981 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     8041 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     8101 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     8161 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     8221 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     8281 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     8341 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     8401 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     8461 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     8521 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     8581 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     8641 tcccaacccc gaggggacaa ttctcgacct cgagacaaat ggcagtattc atccacaatt
     8701 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     8761 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     8821 tttattacag
//
