LOCUS       sequence-22514-e         708 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 708)
  AUTHORS   Zhou BP, Liao Y, Xia W, Spohn B, Lee MH, Hung MC
  TITLE     Cytoplasmic localization of p21Cip1/WAF1 by Akt-induced 
            phosphorylation in HER-2/neu-overexpressing cells.
  JOURNAL   Nat Cell Biol. 2001 Mar . 3(3):245-52.
  PUBMED    11231573
REFERENCE   2  (bases 1 to 708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2001 Mar . 3(3):245-52."
COMMENT     Sequence Label: Flag p21 T145D
FEATURES             Location/Qualifiers
     source          1..708
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             87..109
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             119..142
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             164..658
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLSRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQDSMTDFYHSKRRLIFSKRK
                     P"
ORIGIN
        1 agctcgttta gtgaccgtca gaattcagat ctggtaccac gcgtatcgat aagcttgcat
       61 gcctgcaggt cgactctaga aataattttg tttaacttta agaaggagat ataccatgga
      121 ctacaaagac gatgacgata aagcaagaag agcatctgtg catatgtcag aaccggctgg
      181 ggatgtccgt cagaacccat gcggcagcaa ggcctgccgc cgcctcttcg gcccagtgga
      241 cagcgagcag ctgagccgcg actgtgatgc gctaatggcg ggctgcatcc aggaggcccg
      301 tgagcgatgg aacttcgact ttgtcaccga gacaccactg gagggtgact tcgcctggga
      361 gcgtgtgcgg ggccttggcc tgcccaagct ctaccttccc acggggcccc ggcgaggccg
      421 ggatgagttg ggaggaggca ggcggcctgg cacctcacct gctctgctgc aggggacagc
      481 agaggaagac catgtggacc tgtcactgtc ttgtaccctt gtgcctcgct caggggagca
      541 ggctgaaggg tccccaggtg gacctggaga ctctcagggt cgaaaacggc ggcaggacag
      601 catgacagat ttctaccact ccaaacgccg gctgatcttc tccaagagga agccctaaga
      661 attctaaagc cgaattctgc agatatcgaa ttcctgcagc ccggggga
//
