LOCUS       sequence-225142-        2850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pDONR vector with SUMO1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Aeschbach L, Dion V
  TITLE     Minimizing carry-over PCR contamination in expanded CAG/CTG repeat 
            instability applications.
  JOURNAL   Sci Rep. 2017 Dec 21;7(1):18026. doi: 10.1038/s41598-017-18168-2.
  PUBMED    29269788
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2017-12-21"; volume: "7(1):18026. doi"; pages: " 
            10.1038/s41598-017-18168-2"
COMMENT     Sequence Label: pDONR-SUMO1
FEATURES             Location/Qualifiers
     source          1..2850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(149..176)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(268..354)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     403..425
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     417..434
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     418..434
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    450..549
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(856..955)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(972..991)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(973..991)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(996..1012)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(996..1012)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1114..1133)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1125..1934
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1192..1211)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2081..2669
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2570..2589
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2823..2840
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat acgcgtaccg
      121 ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg ccttctgctt
      181 agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg gccgttgctt
      241 cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt tcaccgacaa
      301 acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta tttgatgcct
      361 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta
      421 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga
      481 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa
      541 aagcaggcta tgtctgacca ggaggcaaaa ccttcaactg aggacttggg ggataagaag
      601 gaaggtgaat atattaaact caaagtcatt ggtcacgaca gtagcgaaat tcacttcaaa
      661 gtgaaaatga caacacatct caagaaactc aaagaatcat actgtcaaag acagggtgtt
      721 ccaatgaatt cactcaggtt tctctttgag ggtcagagaa ttgctgataa tcatactcca
      781 aaagaactcg gtatggaaga ggaggatgtg attgaagttt atcaggaaca aacggggggt
      841 cattcaacag tttagaccca gctttcttgt acaaagttgg cattataaga aagcattgct
      901 tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
      961 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1021 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa
     1081 caataaaact gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac
     1141 gggaaacgtc gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat
     1201 gggctcgcga taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg
     1261 atgcgccaga gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg
     1321 agatggtcag actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta
     1381 tccgtactcc tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc
     1441 aggtattaga agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc
     1501 tgcgccggtt gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc
     1561 gtctcgctca ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg
     1621 acgagcgtaa tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat
     1681 tctcaccgga ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg
     1741 aggggaaatt aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg
     1801 atcttgccat cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt
     1861 ttcaaaaata tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg
     1921 atgagttttt ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga
     1981 cttgacggga cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc
     2041 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     2101 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     2161 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     2221 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     2281 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     2341 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     2401 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     2461 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     2521 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     2581 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     2641 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     2701 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     2761 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     2821 gcagcgagtc agtgagcgag gaagcggaag
//
