LOCUS       sequence-225146-        7985 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human DPP6 variant 1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7985)
  AUTHORS   Lainez S, Doray A, Hancox JC, Cannell MB
  TITLE     Regulation of Kv4.3 and hERG potassium channels by KChIP2 isoforms 
            and DPP6 and response to the dual K(+) channel activator NS3623.
  JOURNAL   Biochem Pharmacol. 2018 Apr;150:120-130. doi: 
            10.1016/j.bcp.2018.01.036. Epub 2018 Jan 31.
  PUBMED    29378180
REFERENCE   2  (bases 1 to 7985)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Pharmacol."; date: "2018-04"; volume: "150:120-130. doi"; pages: " 
            10.1016/j.bcp.2018.01.036. Epub 2018 Jan 31"
COMMENT     Sequence Label: pcDNA3.1+DPP6
FEATURES             Location/Qualifiers
     source          1..7985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      701..710
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(3342..3359)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3348..3572
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3618..4046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3705..3724)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3915..3936
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4055..4075)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4060..4389
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4240..4375
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4302..4321
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4456..5250
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4510..4529)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5120..5139
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5424..5545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5461..5480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5515..5534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5594..5610)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5594..5610)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5607..5629)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5618..5634
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5642..5648,5649..5666,5667..5672))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5687..5708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5825..5842)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5996..6584)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6076..6095)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6755..7546,7547..7615))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7378..7397
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7616..7720)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7795..7814)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagcgttt aaacttaagc ttggtaccga gctcggatcc gccaccatgg cttcgctgta
      721 ccagaggttc actggcaaga tcaacacctc gaggtccttc cccgcgcccc cggaggcgag
      781 tcacctcctg ggcggccagg ggcccgagga ggacggcggc gcaggagcca agcccctcgg
      841 cccgcgggcg caggcggcgg cgccccggga gcgcggcggc ggcggcggcg gcgcgggtgg
      901 ccggccccgg ttccagtacc aggcgcggag cgatggtgac gaggaggacg agctggtggg
      961 gagtaaccct ccgcagagga attggaaagg aatagcaatt gcactgcttg tcattctggt
     1021 catctgctcc ttgatcgtca cctcggtcat acttctgaca ccagcggaag ataatagtct
     1081 gtctcaaaag aagaaggtca ctgtagaaga tctcttcagt gaagacttca aaattcatga
     1141 ccccgaggct aagtggataa gtgatacaga attcatctac agagaacaga aaggaacagt
     1201 gagactgtgg aatgttgaaa caaatacttc tactgtctta atagaaggca aaaaaattga
     1261 atcattaaga gccatcagat atgaaatatc tccagataga gagtatgcac ttttttcata
     1321 caatgtggaa cccatatatc aacactcgta tactggatat tacgtcctga gcaaaattcc
     1381 tcatggggat cctcaaagtc tggacccacc agaagtcagc aatgcaaaac ttcagtatgc
     1441 aggatggggc cctaaaggcc aacagctgat atttattttt gaaaacaata tctactactg
     1501 tgcacatgtc gggaaacagg ccatccgtgt ggtctccact ggcaaggaag gtgtgattta
     1561 caatggcctc agtgactggc tgtatgaaga ggagattttg aagacacaca tcgcacactg
     1621 gtggtctccg gatggcacga gactcgccta cgccgccatc aatgattccc gtgtccccat
     1681 catggagctc ccaacttaca ccggctccat ctaccccacc gtgaagccct accactatcc
     1741 caaggctgga agtgagaacc ccagcatttc cctacacgtt attggcttaa atggacccac
     1801 ccatgatctg gagatgatgc cgcctgatga tccacggatg agggagtact acatcaccat
     1861 ggtgaagtgg gccaccagca ccaaggtcgc cgtgacctgg ctgaaccggg cgcagaacgt
     1921 gtccatcctc accctctgcg acgccaccac gggggtctgc acgaagaaac acgaggatga
     1981 aagtgaggcc tggctccaca gacagaatga agaacctgtg ttctccaagg atggccgaaa
     2041 gtttttcttc atcagagcca tcccccaggg aggacgaggg aaattctatc acatcacggt
     2101 gtcctcgtcc cagcccaaca gcagcaacga caacatccag tccatcacct ccggggactg
     2161 ggacgtgacc aagatcctag cctacgatga gaaggggaat aagatctact tcctgagcac
     2221 ggaggacctg cctcggagac gacaactcta cagtgccaac acggtgggca acttcaacag
     2281 gcagtgcctc tcctgtgacc tggttgagaa ctgcacctac ttcagcgctt ccttcagcca
     2341 tagcatggac ttcttcctgc tcaagtgcga aggtcctggt gttcctatgg tgacggtgca
     2401 caacacaaca gataagaaaa aaatgtttga cctagaaaca aatgaacatg tcaagaaggc
     2461 cataaatgac cgacagatgc ctaaagtgga atacagggac attgagattg atgattacaa
     2521 cctgcccatg cagatactga agccagcaac cttcaccgac accacccact accctctgct
     2581 cctggtggtg gatggcaccc caggcagcca gagtgtggct gagaagttcg aggtgagctg
     2641 ggagacggtg atggtgagca gccacggcgc ggtggtggta aagtgtgacg gccgtggcag
     2701 cggcttccaa gggaccaagc tcctgcacga agtgaggcgg cggctgggct tgctggagga
     2761 gaaggaccag atggaggccg tgcggacgat gctgaaggag cagtacattg acaggacgcg
     2821 cgtggccgtg tttgggaagg attacggtgg ctacctgagc acctacatcc tcccagcaaa
     2881 gggagaaaat caaggccaga cattcacctg cggctctgct ctctctccaa taacagactt
     2941 caaactctat gcctctgcgt tttccgagag gtacttgggc ctccatggac ttgacaacag
     3001 agcatacgag atgaccaagg tagcccatcg agtctccgcg ctggaagaac agcagttcct
     3061 gatcattcat cccactgccg atgaaaaaat tcatttccag cacacagcag aactcattac
     3121 acaactaatt aggggaaagg ctaattacag cttacagatt tacccggacg aaagccatta
     3181 ctttaccagc tccagcctca aacagcatct gtaccggtcc atcatcaact tcttcgtgga
     3241 atgcttcagg atccaggaca aactgctgac agtcacagcg aaagaggacg aggaggagga
     3301 ctaactcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
     3361 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
     3421 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
     3481 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
     3541 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
     3601 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     3661 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     3721 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     3781 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     3841 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     3901 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     3961 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4021 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     4081 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     4141 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     4201 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     4261 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     4321 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     4381 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga
     4441 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg
     4501 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc
     4561 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt
     4621 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt
     4681 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc
     4741 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc
     4801 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac
     4861 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag
     4921 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag
     4981 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat
     5041 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg
     5101 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa
     5161 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc
     5221 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc
     5281 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt
     5341 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca
     5401 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa
     5461 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     5521 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct
     5581 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
     5641 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
     5701 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
     5761 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
     5821 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
     5881 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
     5941 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
     6001 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
     6061 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
     6121 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
     6181 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
     6241 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
     6301 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
     6361 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
     6421 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
     6481 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
     6541 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
     6601 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
     6661 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
     6721 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
     6781 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
     6841 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc
     6901 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
     6961 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
     7021 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
     7081 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
     7141 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
     7201 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     7261 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     7321 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     7381 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     7441 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     7501 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     7561 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     7621 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     7681 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     7741 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat
     7801 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc
     7861 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat
     7921 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg ccagatatac
     7981 gcgtt
//
