LOCUS       sequence-225184-        9934 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Donor vector for creation of TP901-1 attP-flanked platforms for 
            cassette exchange using CRISPR/Cas9.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9934)
  AUTHORS   Voutev R, Mann RS
  TITLE     TP901-1 Phage Recombinase Facilitates Genome Engineering in 
            Drosophila melanogaster.
  JOURNAL   G3 (Bethesda). 2019 Jan 29. pii: g3.119.0002. doi: 
            10.1534/g3.119.0002.
  PUBMED    30696702
REFERENCE   2  (bases 1 to 9934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2019 Jan 29. pii: g3.119.0002. doi: 
            10.1534/g3.119.0002."
COMMENT     Sequence Label: pRVV689
FEATURES             Location/Qualifiers
     source          1..9934
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            665..4801
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(674..692)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1144..1215,1617..1890,1965..2619,2681..2996,3217..3348,
                     3419..4033)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     intron          7099..7164
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7294..7314
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7586..7720
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7623..7642)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7677..7696
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        complement(7746..7764)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7785..7801)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7785..7801)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7798..7820)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7809..7825
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7833..7839,7840..7857,7858..7863))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7878..7899
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8016..8033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8187..8775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8267..8286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8946..9737,9738..9806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9569..9588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9807..9911)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggccctagta tgtatgtaag ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac
      721 attttttttt tttactgcac tggatatcat tgaacttatc tgatcagttt taaatttact
      781 tcgatccaag ggtatttgat gtaccaggtt ctttcgatta cctctcactc aaaatgacat
      841 tccactcaaa gtcagcgctg tttgcctcct tctctgtcca cagaaatatc gccgtctctt
      901 tcgccgctgc gtccgctatc tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc
      961 cgccttcagt tgcattttgt cagcggtttc gtgacgaagc tccaagcggt ttacgccatc
     1021 aattaaacac aaagtgctgt gccaaaactc ctctcgcttc ttatttttgt ttgttttttg
     1081 agtgattggg gtggtgattg gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg
     1141 gcaatgggcc aagaggatca ggagctatta attcgcggag gcagcaaaca cccatctgcc
     1201 gagcatctga acaatgtgag tagtacatgt gcatacatct taagttcact tgatctatag
     1261 gaactgcgat tgcaacatca aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc
     1321 aaaccgcaat tgcacaaaca aatagtgaca cgaaacagat tattctggta gctgttctcg
     1381 ctatataaga caatttttga gatcatatca tgatcaagac atctaaaggc attcattttc
     1441 gactatattc ttttttacaa aaaatataac aaccagatat tttaagctga tcctagatgc
     1501 acaaaaaata aataaaagta taaacctact tcgtaggata cttcggggta ctttttgttc
     1561 ggggttagat gagcataacg cttgtagttg atatttgaga tcccctatca ttgcagggtg
     1621 acagcggagc ggcttcgcag agctgcatta accagggctt cgggcaggcc aaaaactacg
     1681 gcacgctccg gccacccagt ccgccggagg actccggttc agggagcggc caactagccg
     1741 agaacctcac ctatgcctgg cacaatatgg acatctttgg ggcggtcaat cagccgggct
     1801 ccggatggcg gcagctggtc aaccggacac gcggactatt ctgcaacgag cgacacatac
     1861 cggcgcccag gaaacatttg ctcaagaacg gtgagtttct attcgcagtc ggctgatctg
     1921 tgtgaaatct taataaaggg tccaattacc aatttgaaac tcagtttgcg gcgtggccta
     1981 tccgggcgaa cttttggccg tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa
     2041 tgcccttgcc tttcgatcgc cgcagggcat ccaagtatcg ccatccggga tgcgactgct
     2101 caatggccaa cctgtggacg ccaaggagat gcaggccagg tgcgcctatg tccagcagga
     2161 tgacctcttt atcggctccc taacggccag ggaacacctg attttccagg ccatggtgcg
     2221 gatgccacga catctgacct atcggcagcg agtggcccgc gtggatcagg tgatccagga
     2281 gctttcgctc agcaaatgtc agcacacgat catcggtgtg cccggcaggg tgaaaggtct
     2341 gtccggcgga gaaaggaagc gtctggcatt cgcctccgag gcactaaccg atccgccgct
     2401 tctgatctgc gatgagccca cctccggact ggactcattt accgcccaca gcgtcgtcca
     2461 ggtgctgaag aagctgtcgc agaagggcaa gaccgtcatc ctgaccattc atcagccgtc
     2521 ttccgagctg tttgagctct ttgacaagat ccttctgatg gccgagggca gggtagcttt
     2581 cttgggcact cccagcgaag ccgtcgactt cttttcctag tgagttcgat gtgtttatta
     2641 agggtatcta gcattacatt acatctcaac tcctatccag cgtgggtgcc cagtgtccta
     2701 ccaactacaa tccggcggac ttttacgtac aggtgttggc cgttgtgccc ggacgggaga
     2761 tcgagtcccg tgatcggatc gccaagatat gcgacaattt tgctattagc aaagtagccc
     2821 gggatatgga gcagttgttg gccaccaaaa atttggagaa gccactggag cagccggaga
     2881 atgggtacac ctacaaggcc acctggttca tgcagttccg ggcggtcctg tggcgatcct
     2941 ggctgtcggt gctcaaggaa ccactcctcg taaaagtgcg acttattcag acaacggtga
     3001 gtggttccag tggaaacaaa tgatataacg cttacaattc ttggaaacaa attcgctaga
     3061 ttttagttag aattgcctga ttccacaccc ttcttagttt ttttcaatga gatgtatagt
     3121 ttatagtttt gcagaaaata aataaatttc atttaactcg cgaacatgtt gaagatatga
     3181 atattaatga gatgcgagta acattttaat ttgcagatgg ttgccatctt gattggcctc
     3241 atctttttgg gccaacaact cacgcaagtg ggcgtgatga atatcaacgg agccatcttc
     3301 ctcttcctga ccaacatgac ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt
     3361 tagaatacat ttgcatatta ataatttact aactttctaa tgaatcgatt cgatttaggt
     3421 gttcacctca gagctgccag tttttatgag ggaggcccga agtcgacttt atcgctgtga
     3481 cacatacttt ctgggcaaaa cgattgccga attaccgctt tttctcacag tgccactggt
     3541 cttcacggcg attgcctatc cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa
     3601 ctgcctggcg ctggtcactc tggtggccaa tgtgtcaacg tccttcggat atctaatatc
     3661 ctgcgccagc tcctcgacct cgatggcgct gtctgtgggt ccgccggtta tcataccatt
     3721 cctgctcttt ggcggcttct tcttgaactc gggctcggtg ccagtatacc tcaaatggtt
     3781 gtcgtacctc tcatggttcc gttacgccaa cgagggtctg ctgattaacc aatgggcgga
     3841 cgtggagccg ggcgaaatta gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa
     3901 ggtcatcctg gagacgctta acttctccgc cgccgatctg ccgctggact acgtgggtct
     3961 ggccattctc atcgtgagct tccgggtgct cgcatatctg gctctaagac ttcgggcccg
     4021 acgcaaggag tagccgacat atatccgaaa taactgcttg tttttttttt ttaccattat
     4081 taccatcgtg tttactgttt attgccccct caaaaagcta atgtaattat atttgtgcca
     4141 ataaaaacaa gatatgacct atagaataca agtatttccc cttcgaacat ccccacaagt
     4201 agactttgga tttgtcttct aaccaaaaga cttacacacc tgcatacctt acatcaaaaa
     4261 ctcgtttatc gctacataaa acaccgggat atatttttta tatacatact tttcaaatcg
     4321 cgcgccctct tcataattca cctccaccac accacgtttc gtagttgctc tttcgctgtc
     4381 tcccacccgc tctccgcaac acattcacct tttgttcgac gaccttggag cgactgtcgt
     4441 tagttccgcg cgattcggtt cgctcaaatg gttccgagtg gttcatttcg tctcaataga
     4501 aattagtaat aaatatttgt atgtacaatt tatttgctcc aatatatttg tatatatttc
     4561 cctcacagct atatttattc taatttaata ttatgacttt ttaaggtaat tttttgtgac
     4621 ctgttcggag tgattagcgt tacaatttga actgaaagtg acatccagtg tttgttcctt
     4681 gtgtagatgc atctcaaaaa aatggtgggc ataatagtgt tgtttatata tatcaaaaat
     4741 aacaactata ataataagaa tacatttaat ttagaaaatg cttggatttc actggaacta
     4801 ggctagattt aaatgcatgc cctgcaggaa gcttcctagg aggcctagat ctgcggccgc
     4861 tccaactcgc ttaattgcga gtttttattt cgtttatttc aattaaggta actaaaaaac
     4921 tccttttaat taagaagttc ctattcttca aaaggtatag gaacttcccg cgagctcgcc
     4981 cggggatcta attcaattag agactaattc aattagagct aattcaatta ggatccaagc
     5041 ttatcgattt cgaaccctcg accgccggag tataaataga ggcgcttcgt ctacggagcg
     5101 acaattcaat tcaaacaagc aaagtgaaca cgtcgctaag cgaaagctaa gcaaataaac
     5161 aagcgcagct gaacaagcta aacaatcggg cggccgcact agagccggtc gccaccatga
     5221 ggtcttccaa gaatgttatc aaggagttca tgaggtttaa ggttcgcatg gaaggaacgg
     5281 tcaatgggca cgagtttgaa atagaaggcg aaggagaggg gaggccatac gaaggccaca
     5341 ataccgtaaa gcttaaggta accaaggggg gacctttgcc atttgcttgg gatattttgt
     5401 caccacaatt tcagtatgga agcaaggtat atgtcaagca ccctgccgac ataccagact
     5461 ataaaaagct gtcatttcct gaaggattta aatgggaaag ggtcatgaac tttgaagacg
     5521 gtggcgtcgt tactgtaacc caggattcca gtttgcagga tggctgtttc atctacaagg
     5581 tcaagttcat tggcgtgaac tttccttccg atggacctgt tatgcaaaag aagacaatgg
     5641 gctgggaagc cagcactgag cgtttgtatc ctcgtgatgg cgtgttgaaa ggagagattc
     5701 ataaggctct gaagctgaaa gacggtggtc attacctagt tgaattcaaa agtatttaca
     5761 tggcaaagaa gcctgtgcag ctaccagggt actactatgt tgactccaaa ctggatataa
     5821 caagccacaa cgaagactat acaatcgttg agcagtatga aagaaccgag ggacgccacc
     5881 atctgttcct ttagcggcca tcgaattcga gctcgcccac taagcgtcgc gccacttcaa
     5941 cgctcgatgg gagcgtcatt ggtgggcggg gtaaccgtcg aaatcagtgt ttacgcttcc
     6001 aatcgcaaca aaaaattcac tgcaacactg aaaagcatac gaaaacgatg aagattgtac
     6061 gagaaaccat aaagtatttt atccacaaag acacgtatag cagaaaagcc aagttaactc
     6121 ggcgataagt tgtgtacaca agaataaaat cggccagatt cagtgttgtc agaaataaga
     6181 aaaccccact atgtttttct ttgccttttc tttctcccag cgatcattca tttcgtggtg
     6241 aaagaacggg gtcattgcac ggagtttcga ctgcgggaaa gcagagctgc cgttcacttc
     6301 gtctataatt agcgctttct attttccccg attcgggccg ctgctgcgct tttccgcctg
     6361 ctgtttgtgg caagtgtagc agcaggctgt gcacgcagtg tggcatgcac ttggctttcc
     6421 accgttggta tcgattctct gggacgatga gtcattcctt tcggggccac agcataatcg
     6481 ttgccagctc accgaaatgg tgacttcatt tcttaactgc cgtcaagcat gcgattgtac
     6541 atacatacat atttatatat gtacatattt atgtgactat ggtaggtcga tataatagca
     6601 atcaacgcaa gcaaatgtgt cagtcctgct tacaggaacg attctattta gtaattttcg
     6661 ttgtataaag taattatgta tgtatgtaag ccccataaat ctgaaacaat taggcaaaac
     6721 catgcgaagc tgaagttcct attcttcaaa aggtatagga acttcaccgg taaggagttt
     6781 tttagttacc ttaattgaaa taaacgaaat aaaaactcgc aattaagcga gttggaggcg
     6841 cgccgctagc catatgggga ttggtggcga cgactcctgg agcccgtcag tatcggcgga
     6901 attacagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
     6961 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccctcgagg gtacctctag
     7021 aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     7081 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     7141 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     7201 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     7261 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     7321 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     7381 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     7441 attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac
     7501 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca
     7561 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata
     7621 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     7681 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cactagttct agccagcttt
     7741 tgttcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     7801 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7861 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7921 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7981 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8041 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8101 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8161 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8221 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8281 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8341 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8401 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8461 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8521 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8581 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8641 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8701 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8761 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8821 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8881 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8941 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9001 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9061 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9121 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9181 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9241 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9301 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9361 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9421 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9481 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9541 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9601 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9661 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9721 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9781 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9841 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9901 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
