LOCUS       sequence-225204-       13399 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express NuMA(1-705+1700-2115-Delta-NLS)-RFP-Nano from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 13399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK729
FEATURES             Location/Qualifiers
     source          1..13399
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1172..1253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(1370..2116)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(2135..2645)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     polyA_signal    2665..2713
                     /note="synthetic polyadenylation signal"
     misc_feature    2727..2818
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     2767..2786
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        2822..3189
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    2830..2848
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2866..2884
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2902..2920
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2938..2956
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2974..2992
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3010..3028
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3046..3064
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     regulatory      6609..6618
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6615..7346
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             7422..7751
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     polyA_signal    7901..7982
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        8004..8514
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             8551..9570
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             8551..9570
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(9880..9897)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9886..10110
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11211..11231)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11211..11229)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11250..11266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11250..11266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11263..11285)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11274..11290
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11298..11304,11305..11322,11323..11328))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11343..11364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(11652..12240)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11732..11751)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12411..13202,13203..13271))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13034..13053
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13272..13376)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gaagctgagt cagtgagcga ggaagctcgg ggcagtgaaa aaaatgcttt atttgtgaaa
     1201 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     1261 acaattgcat tcattttatg tttcaggttc agggggaggt gtgggaggtt ttttaaagca
     1321 agtaaaacct ctacaaatgt ggtatggctg attatgatcc tctggagatt tacccgggga
     1381 gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg tcaaagtcgt
     1441 caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg gtcggcccgc
     1501 cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg ccgaaaagga
     1561 aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga agtgggggca
     1621 tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc tgttcctcca
     1681 atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg ttctccaggg
     1741 agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac tgtttctctg
     1801 ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga gcacagcggt
     1861 atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg gggcaggagt
     1921 gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc cgcttgttct
     1981 tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt ttccttgtcg
     2041 tcaggccttc gataccgact ccattgagta attccagagc agagtttatg actttgctct
     2101 tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg gtgattcggt
     2161 caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg gaacaccgcg
     2221 cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc gccccttccc
     2281 ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc catcgcggtc
     2341 ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg agacgtgcgg
     2401 cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga cttaggggcg
     2461 gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg gtgacgctgc
     2521 gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc ccggaaccac
     2581 gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg cgcaacccca
     2641 accccgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     2701 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     2761 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     2821 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     2881 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     2941 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     3001 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     3061 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     3121 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     3181 cctcgtaaag aattcgcggc cgcacgcgtg ccaccatgac actccacgcc acccgggggg
     3241 ctgcactcct ctcttgggtg aacagtctac acgtggctga ccctgtggag gctgtgctgc
     3301 agctccagga ctgcagcatc ttcatcaaga tcattgacag aatccatggc actgaagagg
     3361 gacagcaaat cttgaagcag ccggtgtcag agagactgga ctttgtgtgc agttttctgc
     3421 agaaaaatcg aaaacatccc tcttccccag aatgcctggt atctgcacag aaggtgctag
     3481 agggatcaga gctggaactg gcgaagatga ccatgctgct cttataccac tctaccatga
     3541 gctccaaaag tcccagggac tgggaacagt ttgaatataa aattcaggct gagttggctg
     3601 tcattcttaa atttgtgctg gaccatgagg acgggctaaa ccttaatgag gacctagaga
     3661 acttcctaca gaaagctcct gtgccttcta cctgttctag cacattccct gaagagctct
     3721 ccccacctag ccaccaggcc aagagggaga ttcgcttcct agagctacag aaggttgcct
     3781 cctcttccag tgggaacaac tttctctcag gttctccagc ttctcccatg ggtgatatcc
     3841 tgcagacccc acagttccag atgagacggc tgaagaagca gcttgctgat gagagaagta
     3901 atagggatga gctggagctg gagctagctg agaaccgcaa gctcctcacc gagaaggatg
     3961 cacagatagc catgatgcag cagcgcattg accgcctagc cctgctgaat gagaagcagg
     4021 cggccagccc actggagccc aaggagcttg aggagctgcg tgacaagaat gagagcctta
     4081 ccatgcggct gcatgaaacc ctgaagcagt gccaggacct gaagacagag aagagccaga
     4141 tggatcgcaa aatcaaccag ctttcggagg agaatggaga cctttccttt aagctgcggg
     4201 agtttgccag tcatctgcag cagctacagg atgccctcaa tgagctgacg gaggagcaca
     4261 gcaaggccac tcaggagtgg ctagagaagc aggcccagct ggagaaggag ctcagcgcag
     4321 ccctgcagga caagaaatgc cttgaagaga agaacgaaat ccttcaggga aaactttcac
     4381 agctggaaga acacttgtcc cagctgcagg ataacccacc ccaggagaag ggcgaggtgc
     4441 tgggtgatgt cttgcagctg gaaaccttga agcaagaggc agccactctt gctgcaaaca
     4501 acacacagct ccaagccagg gtagagatgc tggagactga gcgaggccag caggaagcca
     4561 agctgcttgc tgagcggggc cacttcgaag aagaaaagca gcagctgtct agcctgatca
     4621 ctgacctgca gagctccatc tccaacctca gccaggccaa ggaagagctg gagcaggcct
     4681 cccaggctca tggggcccgg ttgactgccc aggtggcctc tctgacctct gagctcacca
     4741 cactcaatgc caccatccag caacaggatc aagaactggc tggcctgaag cagcaggcca
     4801 aagagaagca ggcccagcta gcacagaccc tccaacagca agaacaggcc tcccagggcc
     4861 tccgccacca ggtggagcag ctaagcagta gcctgaagca gaaggagcag cagttgaagg
     4921 aggtagcgga gaagcaggag gcaactaggc aggaccatgc ccagcaactg gccactgctg
     4981 cagaggagcg agaggcctcc ttaagggagc gggatgcggc tctcaagcag ctggaggcac
     5041 tggagaagga gaaggctgcc aagctggaga ttctgcagca gcaacttcag gtggctaatg
     5101 aagcccggga cagtgcccag acctcagtga cacaggccca gcgggagaag gcagagctga
     5161 gccggaaggt ggaggaactc caggcctgtg ttgagacagc ccgccaggaa cagcatgagg
     5221 cccaggccca ggttgcagag ctagagttgc agctgcggtc tgagcagcaa aaagcaactg
     5281 agaaagaaag ggtggcccag gagaaggacc agctccagga gcagctccag ggaggtgcca
     5341 ccatgttcca ggtggcaact gatgctttaa agagccgtga gccccaggct aagccccagc
     5401 tggacttgag tattgacagc ctggatctga gctgcgagga ggggacccca ctcagtatca
     5461 ccagcaagct gcctcgtacc cagccagacg gcaccagcgt ccctggagaa ccagcctcac
     5521 ctatctccca gcgcctgccc cccaaggtag aatccctgga gagtctctac ttcactccca
     5581 tccctgctcg gagtcaggcc cccctggaga gcagcctgga ctccctggga gacgtcttcc
     5641 tggactcggg tcgtaagacc cgctccgctc gtcggcgcac cacgcagatc atcaacatca
     5701 ccatgaccaa gaagctagat gtggaagagc cagacagcgc caactcatcg ttctacagca
     5761 cgcggtctgc tcctgcttcc caggctagcc tgcgagccac ctcctctact cagtctctag
     5821 ctcgcctggg ttctcccgat tatggcaact cagccctgct cagcttgcct ggctaccgcc
     5881 ccaccactcg cagttctgct cgtcgttccc aggccggggt gtccagtggg gcccctccag
     5941 gaaggaacag cttctacatg ggcacttgcc aggatgagcc tgagcagctg gatgactgga
     6001 accgcattgc agagctgcag cagcgcaatc gagtgtgccc cccacatctg aagacctgct
     6061 atcccctgga gtccaggcct tccctgagcc tgggcaccat cacagatgag gagatgaaaa
     6121 ctggagaccc ccaagagacc ctgcgccgag ccagcatgca gccaatccag atagccgagg
     6181 gcactggcat caccacccgg gtctccctag agccccacca gggccctgga actcctgagt
     6241 ctaagaaggc caccagctgt ttcccacgcc ccatgactcc ccgagaccga catgaagggc
     6301 gcaaacagag cactactgag gcccagaaga aagcagctcc agcttctact aaacaggctg
     6361 accggcgcca gtcgatggcc ttcagcatcc tcaacacacc caagaagcta gggaacagcc
     6421 ttctgcggcg gggagccyca aagaaggccc tgtccaaggc ttcccccaac acycgcagtg
     6481 gaacccgccg ttctccgcgc attgccacca ccacagccag tgccgccact gctgccgcca
     6541 ttggtgccac ccctcgagcc aagggcaagg caaagcacgg aggtggatct acgcgtggag
     6601 gtggatctgc caccatggtg tctaagggcg aagagctgat taaggagaac atgcacatga
     6661 agctgtacat ggagggcacc gtgaacaacc accacttcaa gtgcacatcc gagggcgaag
     6721 gcaagcccta cgagggcacc cagaccatga gaatcaaggt ggtcgagggc ggccctctcc
     6781 ccttcgcctt cgacatcctg gctaccagct tcatgtacgg cagcagaacc ttcatcaacc
     6841 acacccaggg catccccgac ttctttaagc agtccttccc tgagggcttc acatgggaga
     6901 gagtcaccac atacgaagac gggggcgtgc tgaccgctac ccaggacacc agcctccagg
     6961 acggctgcct catctacaac gtcaagatca gaggggtgaa cttcccatcc aacggccctg
     7021 tgatgcagaa gaaaacactc ggctgggagg ccaacaccga gatgctgtac cccgctgacg
     7081 gcggcctgga aggcagaacc gacatggccc tgaagctcgt gggcgggggc cacctgatct
     7141 gcaacttcaa gaccacatac agatccaaga aacccgctaa gaacctcaag atgcccggcg
     7201 tctactatgt ggaccacaga ctggaaagaa tcaaggaggc cgacaaagag acctacgtcg
     7261 agcagcacga ggtggctgtg gccagatact gcgacctccc tagcaaactg gggcacaaac
     7321 ttaatggcat ggacgagctg tacaagtccg gactcagatc tcgagctcaa gcttcgaacg
     7381 aattcggcat tgatctgagc ggcctgaccc tgcaggaatt cagctccccg aaacgcccta
     7441 agctgctgcg tgaatattac gattggctgg ttgataacag ctttacccca tatctggtgg
     7501 tggatgccac atacctgggc gtgaacgtgc ccgtggagta tgtgaaagac ggtcagatcg
     7561 tgctgaatct gtctgcaagt gcgaccggca acctgcaact gacaaatgat tttatccagt
     7621 tcaacgcccg ctttaagggc gtgtctcgtg aactgtatat cccgatgggt gccgctctgg
     7681 ccatttacgc tcgcgagaac ggcgatggtg tgatgttcga accagaagaa atctatgacg
     7741 agctgaatat tggttaaacc ggtggatcca gatctctgca gcatatgtct agaggatcat
     7801 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     7861 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     7921 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7981 gccgacgtca ggtggcactt ttcggggttg gggttgcgcc ttttccaagg cagccctggg
     8041 tttgcgcagg gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct
     8101 gggtctcgca cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt
     8161 gtgggccccc cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc
     8221 gcggcgtgcc ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga
     8281 cagcgccagg gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc
     8341 tcagcagggc gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg
     8401 gcggtagtgt gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag
     8461 cgcacgtcgg cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat
     8521 ccaccggagc ttaccatgga tagatccgga aagcctgaac tcaccgcgac gtctgtcgag
     8581 aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc ggagggcgaa
     8641 gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc
     8701 tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc ggccgcgctc
     8761 ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta ttgcatctcc
     8821 cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc cgctgttctg
     8881 cagccggtcg cggaggccat ggatgcgatc gctgcggccg atcttagcca gacgagcggg
     8941 ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga tttcatatgc
     9001 gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac cgtcagtgcg
     9061 tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc cgaagtccgg
     9121 cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg ccgcataaca
     9181 gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt cgccaacatc
     9241 ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt cgagcggagg
     9301 catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat tggtcttgac
     9361 caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc gcagggtcga
     9421 tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat cgcccgcaga
     9481 agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc
     9541 cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa gagctgattt
     9601 cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga gagaacggcc
     9661 ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc tgggtcgcgg
     9721 gagggccggt cgcagtgatt caggcccttc tggattgtgt tggtccccag ggcacgattg
     9781 tcatgcccac gcactcgggt gatctgactg atcccgcaga ttggagatcg ccgcccgtgc
     9841 ctgccgattg ggtgcagatc tctagagctc gctgatcagc ctcgactgtg ccttctagtt
     9901 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     9961 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
    10021 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
    10081 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
    10141 cgatgaggga gctgcggtgg aggaggtgga gagaaggccg cacccttctg ggcaggggga
    10201 ggggagtgcc gcaatacctt tatgggagtt ctctgctgcc tcccgtcttg taaggaccgc
    10261 cctgggcctg gaagaagccc tccctccttt cctcctcgcg tgatctcgtc atcgcctcca
    10321 tgtcgagtcg cttctcgatt atgggcggga ttcttttgcc taggcttaag gggctaactt
    10381 ggtccctggg cgttgccctg caggggagtg agcagctgta agatttgagg ggcgactccg
    10441 attagtttat cttcccacgg actagagttg gtgtcgaggt tattgtaata agggtggggt
    10501 agggaaatgg agcttagtca ttcacctggg gctgatttta tgcaacgaga ctgcggatta
    10561 tcactaatca tttttggagc atttttctag agacagacat aaagcatgat cacctgagtt
    10621 ttataccatt tgagaccctt gctgcaccac caaagtgtag catcaggtta aatcttaata
    10681 gaaaaatttt agcttttgct tgagaaacca gtgcttccct ccctcaccct ctctccccag
    10741 gctctctacc cctttgcatc cctaccaggc atcttagcaa ctctcactca tacttgatcc
    10801 cattttccat ttgttgtact tgctcctcca gtattcagac atagcactag ctttctccct
    10861 ctcttgatct tgggtagcct ggtgtctcgc gaaaccagac agattggttc caccacaaat
    10921 taaggcttga gctggggctt gactcttacc cagcagtgct tttattcctc cctagttcac
    10981 gttcttaaat gtttatcttg attttcattt tatccttttt ccttagctgg gattctgtcc
    11041 ctgaccgtct tcacagtcca ggtgatcttg actactgctt tacagagaat tggatctgag
    11101 gttaggcaac atctcccttt ttcttcctct aaatacctct catttctgtt cttaccagtt
    11161 agtaactgat ctcagatgcc tgtgtgatag ctggtaccca gcttttgttc cctttagtga
    11221 gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    11281 ccgctcacaa ttccacacaa catacgagcc gggagcataa agtgtaaagc ctggggtgcc
    11341 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    11401 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    11461 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
    11521 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
    11581 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
    11641 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
    11701 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
    11761 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
    11821 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
    11881 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
    11941 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
    12001 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
    12061 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
    12121 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
    12181 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
    12241 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
    12301 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
    12361 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
    12421 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
    12481 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
    12541 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
    12601 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
    12661 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    12721 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    12781 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    12841 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    12901 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    12961 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    13021 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    13081 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    13141 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    13201 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    13261 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    13321 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    13381 tttccccgaa aagtgccac
//
