LOCUS       sequence-225213-        8341 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA for LGN's N-terminal locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK473
FEATURES             Location/Qualifiers
     source          1..8341
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      221..230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             230..295
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             302..322
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             347..4447
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             347..4447
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4448..4495
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(4523..4540)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4529..4736
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4745..4885
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4745..4874
                     /label=AAV2 ITR
     rep_origin      4960..5415
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5047..5066)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5257..5278
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5432..5451)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5551..5573
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5611..5629)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5697..5801
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5802..5870,5871..6662)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6020..6039)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6833..7421
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7322..7341
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7483..7731
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7483..7503
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7654..7673
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7753..7828
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        7925..8210
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc gccccggctc tgactgaccg
       61 cgttactccc acaggtgagc gggcgggacg gcccttctcc tccgggctgt aattagctga
      121 gcaagaggta agggtttaag ggatggttgg ttggtggggt attaatgttt aattacctgg
      181 agcacctgcc tgaaatcact ttttttcagg ttggaccggt gccaccatgg actataagga
      241 ccacgacgga gactacaagg atcatgatat tgattacaaa gacgatgacg ataagatggc
      301 cccaaagaag aagcggaagg tcggtatcca cggagtccca gcagccgaca agaagtacag
      361 catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg acgagtacaa
      421 ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca tcaagaagaa
      481 cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc ggctgaagag
      541 aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc aagagatctt
      601 cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag agtccttcct
      661 ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg tggacgaggt
      721 ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg tggacagcac
      781 cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca agttccgggg
      841 ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca agctgttcat
      901 ccagctggtg cagacctaca accagctgtt cgaggaaaac cccatcaacg ccagcggcgt
      961 ggacgccaag gccatcctgt ctgccagact gagcaagagc agacggctgg aaaatctgat
     1021 cgcccagctg cccggcgaga agaagaatgg cctgttcgga aacctgattg ccctgagcct
     1081 gggcctgacc cccaacttca agagcaactt cgacctggcc gaggatgcca aactgcagct
     1141 gagcaaggac acctacgacg acgacctgga caacctgctg gcccagatcg gcgaccagta
     1201 cgccgacctg tttctggccg ccaagaacct gtccgacgcc atcctgctga gcgacatcct
     1261 gagagtgaac accgagatca ccaaggcccc cctgagcgcc tctatgatca agagatacga
     1321 cgagcaccac caggacctga ccctgctgaa agctctcgtg cggcagcagc tgcctgagaa
     1381 gtacaaagag attttcttcg accagagcaa gaacggctac gccggctaca ttgacggcgg
     1441 agccagccag gaagagttct acaagttcat caagcccatc ctggaaaaga tggacggcac
     1501 cgaggaactg ctcgtgaagc tgaacagaga ggacctgctg cggaagcagc ggaccttcga
     1561 caacggcagc atcccccacc agatccacct gggagagctg cacgccattc tgcggcggca
     1621 ggaagatttt tacccattcc tgaaggacaa ccgggaaaag atcgagaaga tcctgacctt
     1681 ccgcatcccc tactacgtgg gccctctggc caggggaaac agcagattcg cctggatgac
     1741 cagaaagagc gaggaaacca tcaccccctg gaacttcgag gaagtggtgg acaagggcgc
     1801 ttccgcccag agcttcatcg agcggatgac caacttcgat aagaacctgc ccaacgagaa
     1861 ggtgctgccc aagcacagcc tgctgtacga gtacttcacc gtgtataacg agctgaccaa
     1921 agtgaaatac gtgaccgagg gaatgagaaa gcccgccttc ctgagcggcg agcagaaaaa
     1981 ggccatcgtg gacctgctgt tcaagaccaa ccggaaagtg accgtgaagc agctgaaaga
     2041 ggactacttc aagaaaatcg agtgcttcga ctccgtggaa atctccggcg tggaagatcg
     2101 gttcaacgcc tccctgggca cataccacga tctgctgaaa attatcaagg acaaggactt
     2161 cctggacaat gaggaaaacg aggacattct ggaagatatc gtgctgaccc tgacactgtt
     2221 tgaggacaga gagatgatcg aggaacggct gaaaacctat gcccacctgt tcgacgacaa
     2281 agtgatgaag cagctgaagc ggcggagata caccggctgg ggcaggctga gccggaagct
     2341 gatcaacggc atccgggaca agcagtccgg caagacaatc ctggatttcc tgaagtccga
     2401 cggcttcgcc aacagaaact tcatgcagct gatccacgac gacagcctga cctttaaaga
     2461 ggacatccag aaagcccagg tgtccggcca gggcgatagc ctgcacgagc acattgccaa
     2521 tctggccggc agccccgcca ttaagaaggg catcctgcag acagtgaagg tggtggacga
     2581 gctcgtgaaa gtgatgggcc ggcacaagcc cgagaacatc gtgatcgaaa tggccagaga
     2641 gaaccagacc acccagaagg gacagaagaa cagccgcgag agaatgaagc ggatcgaaga
     2701 gggcatcaaa gagctgggca gccagatcct gaaagaacac cccgtggaaa acacccagct
     2761 gcagaacgag aagctgtacc tgtactacct gcagaatggg cgggatatgt acgtggacca
     2821 ggaactggac atcaaccggc tgtccgacta cgatgtggac catatcgtgc ctcagagctt
     2881 tctgaaggac gactccatcg acaacaaggt gctgaccaga agcgacaaga accggggcaa
     2941 gagcgacaac gtgccctccg aagaggtcgt gaagaagatg aagaactact ggcggcagct
     3001 gctgaacgcc aagctgatta cccagagaaa gttcgacaat ctgaccaagg ccgagagagg
     3061 cggcctgagc gaactggata aggccggctt catcaagaga cagctggtgg aaacccggca
     3121 gatcacaaag cacgtggcac agatcctgga ctcccggatg aacactaagt acgacgagaa
     3181 tgacaagctg atccgggaag tgaaagtgat caccctgaag tccaagctgg tgtccgattt
     3241 ccggaaggat ttccagtttt acaaagtgcg cgagatcaac aactaccacc acgcccacga
     3301 cgcctacctg aacgccgtcg tgggaaccgc cctgatcaaa aagtacccta agctggaaag
     3361 cgagttcgtg tacggcgact acaaggtgta cgacgtgcgg aagatgatcg ccaagagcga
     3421 gcaggaaatc ggcaaggcta ccgccaagta cttcttctac agcaacatca tgaacttttt
     3481 caagaccgag attaccctgg ccaacggcga gatccggaag cggcctctga tcgagacaaa
     3541 cggcgaaacc ggggagatcg tgtgggataa gggccgggat tttgccaccg tgcggaaagt
     3601 gctgagcatg ccccaagtga atatcgtgaa aaagaccgag gtgcagacag gcggcttcag
     3661 caaagagtct atcctgccca agaggaacag cgataagctg atcgccagaa agaaggactg
     3721 ggaccctaag aagtacggcg gcttcgacag ccccaccgtg gcctattctg tgctggtggt
     3781 ggccaaagtg gaaaagggca agtccaagaa actgaagagt gtgaaagagc tgctggggat
     3841 caccatcatg gaaagaagca gcttcgagaa gaatcccatc gactttctgg aagccaaggg
     3901 ctacaaagaa gtgaaaaagg acctgatcat caagctgcct aagtactccc tgttcgagct
     3961 ggaaaacggc cggaagagaa tgctggcctc tgccggcgaa ctgcagaagg gaaacgaact
     4021 ggccctgccc tccaaatatg tgaacttcct gtacctggcc agccactatg agaagctgaa
     4081 gggctccccc gaggataatg agcagaaaca gctgtttgtg gaacagcaca agcactacct
     4141 ggacgagatc atcgagcaga tcagcgagtt ctccaagaga gtgatcctgg ccgacgctaa
     4201 tctggacaaa gtgctgtccg cctacaacaa gcaccgggat aagcccatca gagagcaggc
     4261 cgagaatatc atccacctgt ttaccctgac caatctggga gcccctgccg ccttcaagta
     4321 ctttgacacc accatcgacc ggaagaggta caccagcacc aaagaggtgc tggacgccac
     4381 cctgatccac cagagcatca ccggcctgta cgagacacgg atcgacctgt ctcagctggg
     4441 aggcgacaaa aggccggcgg ccacgaaaaa ggccggccag gcaaaaaaga aaaagtaaga
     4501 attcctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     4561 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     4621 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     4681 gtggggcagg acagcaaggg ggaggattgg gaagagaata gcaggcatgc tggggagcgg
     4741 ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg ctcgctcact
     4801 gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc
     4861 gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct tacgcatctg
     4921 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
     4981 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccttag
     5041 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5101 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5161 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5221 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5281 caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
     5341 tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     5401 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     5461 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     5521 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     5581 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
     5641 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
     5701 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5761 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5821 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5881 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5941 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     6001 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     6061 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     6121 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     6181 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     6241 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     6301 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     6361 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     6421 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     6481 ggtttattgc tgataaatct ggagccggtg agcgtggaag ccgcggtatc attgcagcac
     6541 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6601 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6661 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6721 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6781 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6841 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6901 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6961 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7021 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7081 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7141 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7201 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     7261 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     7321 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     7381 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     7441 ttttacggtt cctggccttt tgctggcctt ttgctcacat gtgagggcct atttcccatg
     7501 attccttcat atttgcatat acgatacaag gctgttagag agataattgg aattaatttg
     7561 actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg
     7621 tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga
     7681 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac cgcttataat
     7741 atgactcgat gggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     7801 acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta gagctagaaa tagcaagtta
     7861 aaataaggct agtccgtttt tagcgcgtgc gccaattctg cagacaaatg gctctagagg
     7921 tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     7981 cattgacgtc aatagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8041 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     8101 ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg
     8161 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     8221 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
     8281 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc
     8341 c
//
