LOCUS       sequence-225225-        9444 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For PYL-HDAC5 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9444)
  AUTHORS   Yang B, Borgeaud AC, Buricova M, Aeschbach L, Rodriguez-Lima O, Ruiz
            Buendia GA, Cinesi C, Taylor AS, Baubec T, Dion V
  TITLE     Expanded CAG/CTG repeats resist gene silencing mediated by targeted 
            Epigenome editing.
  JOURNAL   Hum Mol Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255.
  PUBMED    34494094
REFERENCE   2  (bases 1 to 9444)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Mol 
            Genet. 2021 Sep 7. pii: 6365982. doi: 10.1093/hmg/ddab255."
COMMENT     Sequence Label: pAB(EXPR-PYL-HDAC5-NEO)
FEATURES             Location/Qualifiers
     source          1..9444
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      913..922
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1456..1527
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags"
                     /label=3xFLAG
                     /translation="DYKDDDDKDYKDDDDKDYKDDDDK"
     CDS             1531..1551
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1552..1576
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4948..4972)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        complement(4997..5015)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4998..5015)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5035..5052)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5041..5265
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5311..5739
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5398..5417)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5608..5629
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5748..5768)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5753..6082
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5933..6068
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5995..6014
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6149..6943
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6203..6222)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6813..6832
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7117..7238
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7154..7173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7208..7227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7287..7303)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7287..7303)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7300..7322)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7311..7327
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7335..7341,7342..7359,7360..7365))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7380..7401
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7518..7535)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7689..8277)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7769..7788)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8448..9239,9240..9308))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9071..9090
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9309..9413)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccgccaccat ggccacccag gacgagttca cccagctgag ccagtctatc
      961 gccgagttcc acacctacca gctgggcaac ggccggtgca gcagcctgct ggcccagaga
     1021 atccacgccc ctcccgagac agtgtggtcc gtcgtgcgga gattcgaccg gccccagatc
     1081 tacaagcact tcatcaagag ctgcaacgtg tccgaggact tcgagatgag agtgggctgc
     1141 acccgggacg tgaacgtgat cagcggcctg cccgccaaca ccagcagaga gagactggac
     1201 ctgctggacg acgacagaag agtgaccggc ttcagcatca ccggcggaga gcaccggctg
     1261 cggaactaca agagcgtgac caccgtgcac agattcgaga aagaggaaga agaggaacgg
     1321 atctggaccg tggtgctgga aagctacgtg gtggacgtgc ccgagggcaa cagcgaagag
     1381 gacacccggc tgttcgccga caccgtgatc cggctgaacc tgcagaagct ggcctccatc
     1441 accgaggcca tgaacgacta caaggacgac gacgacaagg actataagga cgatgatgat
     1501 aaggattaca aagatgacga cgataagccc cccaagaaga agcggaaagt cacaagtttg
     1561 tacaaaaaag caggctcaat gaactctccc aacgagtcgg atgggatgtc aggtcgggaa
     1621 ccatccttgg aaatcctgcc gcggacttct ctgcacagca tccctgtgac agtggaggtg
     1681 aagccggtgc tgccaagagc catgcccagt tccatggggg gtgggggtgg aggcagcccc
     1741 agccctgtgg agctacgggg ggctctggtg ggctctgtgg accccacact gcgggagcag
     1801 caactgcagc aggagctcct ggcgctcaag cagcagcagc agctgcagaa gcagctcctg
     1861 ttcgctgagt tccagaaaca gcatgaccac ctgacaaggc agcatgaggt ccagctgcag
     1921 aagcacctca agcagcagca ggagatgctg gcagccaagc agcagcagga gatgctggca
     1981 gccaagcggc agcaggagct ggagcagcag cggcagcggg agcagcagcg gcaggaagag
     2041 ctggagaagc agcggctgga gcagcagctg ctcatcctgc ggaacaagga gaagagcaaa
     2101 gagagtgcca ttgccagcac tgaggtaaag ctgaggctcc aggaattcct cttgtcgaag
     2161 tcaaaggagc ccacaccagg cggcctcaac cattccctcc cacagcaccc caaatgctgg
     2221 ggagcccacc atgcttcttt ggaccagagt tcccctcccc agagcggccc ccctgggacg
     2281 cctccctcct acaaactgcc tttgcctggg ccctacgaca gtcgagacga cttccccctc
     2341 cgcaaaacag cctctgaacc caacttgaaa gtgcgttcaa ggctaaaaca gaaggtggct
     2401 gagcggagaa gcagtcccct cctgcgtcgc aaggatggga ctgttattag cacctttaag
     2461 aagagagctg ttgagatcac aggtgccggg cctggggcgt cgtccgtgtg taacagcgca
     2521 cccggctccg gccccagctc tcccaacagc tcccacagca ccatcgctga gaatggcttt
     2581 actggctcag tccccaacat ccccactgag atgctccctc agcaccgagc cctccctctg
     2641 gacagctccc ccaaccagtt cagcctctac acgtctcctt ctctgcccaa catctcccta
     2701 gggctgcagg ccacggtcac tgtcaccaac tcacacctca ctgcctcccc gaagctgtcg
     2761 acacagcagg aggccgagag gcaggccctc cagtccctgc ggcagggtgg cacgctgacc
     2821 ggcaagttca tgagcacatc ctctattcct ggctgcctgc tgggcgtggc actggagggc
     2881 gacgggagcc cccacgggca tgcctccctg ctgcagcatg tgctgttgct ggagcaggcc
     2941 cggcagcaga gcaccctcat tgctgtgcca ctccacgggc agtccccact agtgacgggt
     3001 gaacgtgtgg ccaccagcat gcggacggta ggcaagctcc cgcggcatcg gcccctgagc
     3061 cgcactcagt cctcaccgct gccgcagagt ccccaggccc tgcagcagct ggtcatgcaa
     3121 caacagcacc agcagttcct ggagaagcag aagcagcagc agctacagct gggcaagatc
     3181 ctcaccaaga caggggagct gcccaggcag cccaccaccc accctgagga gacagaggag
     3241 gagctgacgg agcagcagga ggtcttgctg ggggagggag ccctgaccat gccccgggag
     3301 ggctccacag agagtgagag cacacaggaa gacctggagg aggaggacga ggaagaggat
     3361 ggggaggagg aggaggattg catccaggtt aaggacgagg agggcgagag tggtgctgag
     3421 gaggggcccg acttggagga gcctggtgct ggatacaaaa aactgttctc agatgcccag
     3481 ccgctgcagc ctttgcaggt gtaccaggcg cccctcagcc tggccactgt gccccaccag
     3541 gccctgggcc gtacccagtc ctcccctgct gcccctgggg gcatgaagag ccccccagac
     3601 cagcccgtca agcacctctt caccacaggt gtggtctacg acacgttcat gctaaagcac
     3661 cagtgcatgt gcgggaacac acacgtgcac cctgagcatg ctggccggat ccagagcatc
     3721 tggtcccggc tgcaggagac aggcctgctt agcaagtgcg agcggatccg aggtcgcaaa
     3781 gccacgctag atgagatcca gacagtgcac tctgaatacc acaccctgct ctatgggacc
     3841 agtcccctca accggcagaa gctagacagc aagaagttgc tcggccccat cagccagaag
     3901 atgtatgctg tgctgccttg tgggggcatc ggggtggaca gtgacaccgt gtggaatgag
     3961 atgcactcct ccagtgctgt gcgcatggca gtgggctgcc tgctggagct ggccttcaag
     4021 gtggctgcag gagagctcaa gaatggattt gccatcatcc ggcccccagg acaccacgcc
     4081 gaggaatcca cagccatggg attctgcttc ttcaactctg tagccatcac cgcaaaactc
     4141 ctacagcaga agttgaacgt gggcaaggtc ctcatcgtgg actgggacat tcaccatggc
     4201 aatggcaccc agcaggcgtt ctacaatgac ccctctgtgc tctacatctc tctgcatcgc
     4261 tatgacaacg ggaacttctt tccaggctct ggggctcctg aagaggttgg tggaggacca
     4321 ggcgtggggt acaatgtgaa cgtggcatgg acaggaggtg tggacccccc cattggagac
     4381 gtggagtacc ttacagcctt caggacagtg gtgatgccca ttgcccacga gttctcacct
     4441 gatgtggtcc tagtctccgc cgggtttgat gctgttgaag gacatctgtc tcctctgggt
     4501 ggctactctg tcaccgccag atgttttggc cacttgacca ggcagctgat gaccctggca
     4561 gggggccggg tggtgctggc cctggaggga ggccatgact tgaccgccat ctgtgatgcc
     4621 tctgaggctt gtgtctcggc tctgctcagt gtagagctgc agcccttgga tgaggcagtc
     4681 ttgcagcaaa agcccaacat caacgcagtg gccacgctag agaaagtcat cgagatccag
     4741 agcaaacact ggagctgtgt gcagaagttc gccgctggtc tgggccggtc cctgcgagag
     4801 gcccaagcag gtgagaccga ggaggccgag actgtgagcg ccatggcctt gctgtcggtg
     4861 ggggccgagc aggcccaggc tgcggcagcc cgggaacaca gccccaggcc ggcagaggag
     4921 cccatggagc aggagcctgc cctgtgaacc cagctttctt gtacaaagtg gtcatccgcg
     4981 gtctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
     5041 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     5101 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     5161 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     5221 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     5281 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     5341 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     5401 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     5461 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     5521 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5581 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5641 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5701 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     5761 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5821 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     5881 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     5941 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6001 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     6061 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     6121 tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc acgcaggttc
     6181 tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg
     6241 ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac
     6301 cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc
     6361 cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg
     6421 gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga
     6481 gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg
     6541 cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg
     6601 tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt
     6661 cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc
     6721 ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg
     6781 gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga
     6841 gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc
     6901 gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc
     6961 gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc
     7021 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     7081 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     7141 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     7201 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     7261 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     7321 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     7381 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     7441 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7501 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7561 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7621 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7681 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7741 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7801 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7861 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7921 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7981 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     8041 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     8101 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     8161 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     8221 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     8281 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     8341 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     8401 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     8461 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     8521 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     8581 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     8641 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     8701 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     8761 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     8821 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     8881 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     8941 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     9001 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     9061 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     9121 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     9181 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     9241 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     9301 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     9361 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     9421 ccccgaaaag tgccacctga cgtc
//
