LOCUS       sequence-225234-         590 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  A vector backbone for ATMT (Agrobacterium tumerfaciens-mediated 
            transformation). There is a 68bp MCS (multiple cloning site) which 
            contains 12 unique restriction sites..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1252
FEATURES             Location/Qualifiers
     source          1..590
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    101..275
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(303..319)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(303..319)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(347..364)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(347..363)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(356..378)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    566..590
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa cacattgcgg
       61 acgtttttat gtactgaatt aacgccgatg taattcgggg gatctggatt ttagtactgg
      121 attttggttt taggaattag aaattttatt gatagaagta ttttacaaat acaaatacat
      181 actaagggtt tcttatatgc tcaacacatg agcgaaaccc tataggaacc ctaattccct
      241 tatctgggaa ctactcacac attattatgg agaaactcga gaattcgagc tcggtacccg
      301 gggatccact agttctagag tcgacctgca ggcatgcaag cttggcactg gccgtcgttt
      361 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
      421 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
      481 tgcgcagcct gaatggcgaa tgctagagca gcttgagctt ggatcagatt gtcgtttccc
      541 gccttcagtt taaactatca gtgtttgaca ggatatattg gcgggtaaac
//
