LOCUS       sequence-22525-e         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Takahashi Y, Toh-E A, Kikuchi Y
  TITLE     Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in 
            vitro.
  JOURNAL   J Biochem (Tokyo). 2003 Apr . 133(4):415-22.
  PUBMED    12761287
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biochem 
            (Tokyo). 2003 Apr . 133(4):415-22."
COMMENT     Sequence Label: pT-77
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             15..37
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..77
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 ccctctagaa taattttgtt taactttaag aaggagatat acatatggct agcatgactg
       61 gtggacagca aatgggtcgg gatccgaatt ccatgataaa tttagaggat tactgggaag
      121 atgaaacgcc tgggcctgat agggaaccga ctaatgaact aagaaacgag gtggaggaaa
      181 ccatcactct gatggaactg ctgaaagtgt ccgaattgaa ggatatttgc aggtctgtat
      241 ctttcccggt ttccggtcgc aaggccgttc tacaggacct tatcaggaat ttcttgcaga
      301 acgctttagt agtaggcaag tctgatcctt atagagttca agccgttaaa tttctgatag
      361 aacggatcag aaagaatgaa cctttgcctg tttataaaga tctttggaac gctttaagaa
      421 agggtacccc attgagtgct atcacagtac gttctatgga ggggcctccc acggtgcagc
      481 agcaatcacc ttccgttatt aggcagtctc ccactcaacg gcggaaaacg tctaccacat
      541 cttccacatc tcgtgcacca ccacctacaa atccagatgc ctcttcttct tcatcatctt
      601 ttgctgtacc gacaattcat tttaaagagt ctccatttta caaaatacaa aggctaatcc
      661 ctgaattagt gatgaacgtg gaagtcacag gcggaagagg tatgtgctct gcgaaattta
      721 aattgtccaa agcagactat aatctattaa gtaacccgaa tag
//
