LOCUS       sequence-22528-e         766 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 766)
  AUTHORS   Takahashi Y, Toh-E A, Kikuchi Y
  TITLE     Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in 
            vitro.
  JOURNAL   J Biochem (Tokyo). 2003 Apr . 133(4):415-22.
  PUBMED    12761287
REFERENCE   2  (bases 1 to 766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biochem 
            (Tokyo). 2003 Apr . 133(4):415-22."
COMMENT     Sequence Label: pT-60
FEATURES             Location/Qualifiers
     source          1..766
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(572..594)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(739..757)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cggatctggt tccgcgtgga tccatgagta gtttgtgtct acagcgtctt caggaagaaa
       61 gaaaaaaatg gagaaaggat catccatttg gattttatgc caaaccagtt aagaaagctg
      121 atgggtccat ggatttacag aaatgggaag ctggtatccc aggcaaagaa ggtacaaact
      181 gggcgggtgg tgtgtaccca attacagtcg aatatccaaa tgaatatcct tcaaaacctc
      241 caaaggttaa atttccagcc ggattttatc atccaaacgt gtatccaagt ggcacaatat
      301 gtttaagtat tttaaatgaa gatcaagatt ggagacccgc catcacgtta aaacaaattg
      361 ttcttggggt tcaggatctt ttagactctc caaatccaaa ttcccctgct caagagcctg
      421 catggagatc attttcaaga aataaggcgg aatatgacaa gaaagttttg cttcaagcta
      481 aacagtactc taaagtcgac tcgagctcaa gcttaattca tcgtgactga ctgacgatct
      541 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
      601 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
      661 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata
      721 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggt
//
