LOCUS       sequence-22529-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Takahashi Y, Toh-E A, Kikuchi Y
  TITLE     Comparative analysis of yeast PIAS-type SUMO ligases in vivo and in 
            vitro.
  JOURNAL   J Biochem (Tokyo). 2003 Apr . 133(4):415-22.
  PUBMED    12761287
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biochem 
            (Tokyo). 2003 Apr . 133(4):415-22."
COMMENT     Sequence Label: pT-61
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             26..48
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             56..88
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 gtngcgnaca ttcccctctg aatattttgt ttaactttaa gaaggagata tacatatggc
       61 tagcatgact ggtggacagc aaatgggtcg ggatccgaat tccatggcaa gtgtcatgtc
      121 aaataataat aataataata ataataataa tgccagttat atgtttacga accctttgtc
      181 caacacaggt ggtggactca taaatgaaat aaaagatgca ataaatgaaa tggaacaatt
      241 aaaggttcta gagttaaagc aaatatgcaa atctctggat ttatccataa cgggcaaaaa
      301 ggctgtctta caagaccgaa taaaacaatt cttaagaaaa tcctgtgata ttggacatat
      361 agatccgtgg aggcctaagg ctattaagat tcttatcgcg aaagtaagga taaactcatc
      421 attaccaaaa tactctaccc tttgggaaac tttaaagacg ggtgcgttca agcatcctgt
      481 agcatcgggg caactacccg tgacagctct ccagagtact gcattgccac cttattccca
      541 acaacaggcc ttagcatatt cttttacctc accgttttac aaaccaatag tacaaatacc
      601 ggatgcccgn taaanaantt gaagcaatct gcaggaacag gntgtacaaa aatgaagtnc
      661 aacgtaagta aatctaatca tgatcttttg aatctaacaa gggtataaac tgtatttgtt
      721 gtctggcttt caataccgnt tatatatgaa actgtgggcn tgagcataga ttnccgtatc
      781 ctgtgactag tgttatggcn caactagaga cacggaaggt taagacaatg ggcggnnctg
      841 cattacnctn ctagccgccg aagacctnct ctncnatgna ngc
//
