LOCUS       sequence-225309-       14456 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mCherry-NuMA(WT) from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14456)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 14456)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK503
FEATURES             Location/Qualifiers
     source          1..14456
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1165..1213
                     /note="synthetic polyadenylation signal"
     misc_feature    1227..1318
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1267..1286
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1322..1689
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1366..1384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1402..1420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1438..1456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1474..1492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1510..1528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1546..1564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1710..2417
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1855..1873)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2123..2142
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8958..9039
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8995..9014)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9061..9571
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9490..9508
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9608..10627
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             9608..10627
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(10937..10954)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10943..11167
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12268..12288)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12268..12286)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12307..12323)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12307..12323)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12320..12342)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12331..12347
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12355..12361,12362..12379,12380..12385))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12400..12421
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12538..12555)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12709..13297)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12789..12808)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13468..14259,14260..14328))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14091..14110
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14329..14433)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gcgttgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     1201 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     1261 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     1321 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     1381 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     1441 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     1501 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     1561 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     1621 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     1681 cctcgtaaag aattcgcggc cgcacgcgta tggtgagcaa gggcgaggag gataacatgg
     1741 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1801 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1861 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1921 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1981 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     2041 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     2101 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2161 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     2221 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     2281 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2341 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2401 tggacgagct gtacaagtcc ggaggggatc ggtggagctc caccggagga ggtggatctg
     2461 tcgacatgac actccacgcc acccgggggg ctgcactcct ctcttgggtg aacagtctac
     2521 acgtggctga ccctgtggag gctgtgctgc agctccagga ctgcagcatc ttcatcaaga
     2581 tcattgacag aatccatggc actgaagagg gacagcaaat cttgaagcag ccggtgtcag
     2641 agagactgga ctttgtgtgc agttttctgc agaaaaatcg aaaacatccc tcttccccag
     2701 aatgcctggt atctgcacag aaggtgctag agggatcaga gctggaactg gcgaagatga
     2761 ccatgctgct cttataccac tctaccatga gctccaaaag tcccagggac tgggaacagt
     2821 ttgaatataa aattcaggct gagttggctg tcattcttaa atttgtgctg gaccatgagg
     2881 acgggctaaa ccttaatgag gacctagaga acttcctaca gaaagctcct gtgccttcta
     2941 cctgttctag cacattccct gaagagctct ccccacctag ccaccaggcc aagagggaga
     3001 ttcgcttcct agagctacag aaggttgcct cctcttccag tgggaacaac tttctctcag
     3061 gttctccagc ttctcccatg ggtgatatcc tgcagacccc acagttccag atgagacggc
     3121 tgaagaagca gcttgctgat gagagaagta atagggatga gctggagctg gagctagctg
     3181 agaaccgcaa gctcctcacc gagaaggatg cacagatagc catgatgcag cagcgcattg
     3241 accgcctagc cctgctgaat gagaagcagg cggccagccc actggagccc aaggagcttg
     3301 aggagctgcg tgacaagaat gagagcctta ccatgcggct gcatgaaacc ctgaagcagt
     3361 gccaggacct gaagacagag aagagccaga tggatcgcaa aatcaaccag ctttcggagg
     3421 agaatggaga cctttccttt aagctgcggg agtttgccag tcatctgcag cagctacagg
     3481 atgccctcaa tgagctgacg gaggagcaca gcaaggccac tcaggagtgg ctagagaagc
     3541 aggcccagct ggagaaggag ctcagcgcag ccctgcagga caagaaatgc cttgaagaga
     3601 agaacgaaat ccttcaggga aaactttcac agctggaaga acacttgtcc cagctgcagg
     3661 ataacccacc ccaggagaag ggcgaggtgc tgggtgatgt cttgcagctg gaaaccttga
     3721 agcaagaggc agccactctt gctgcaaaca acacacagct ccaagccagg gtagagatgc
     3781 tggagactga gcgaggccag caggaagcca agctgcttgc tgagcggggc cacttcgaag
     3841 aagaaaagca gcagctgtct agcctgatca ctgacctgca gagctccatc tccaacctca
     3901 gccaggccaa ggaagagctg gagcaggcct cccaggctca tggggcccgg ttgactgccc
     3961 aggtggcctc tctgacctct gagctcacca cactcaatgc caccatccag caacaggatc
     4021 aagaactggc tggcctgaag cagcaggcca aagagaagca ggcccagcta gcacagaccc
     4081 tccaacagca agaacaggcc tcccagggcc tccgccacca ggtggagcag ctaagcagta
     4141 gcctgaagca gaaggagcag cagttgaagg aggtagcgga gaagcaggag gcaactaggc
     4201 aggaccatgc ccagcaactg gccactgctg cagaggagcg agaggcctcc ttaagggagc
     4261 gggatgcggc tctcaagcag ctggaggcac tggagaagga gaaggctgcc aagctggaga
     4321 ttctgcagca gcaacttcag gtggctaatg aagcccggga cagtgcccag acctcagtga
     4381 cacaggccca gcgggagaag gcagagctga gccggaaggt ggaggaactc caggcctgtg
     4441 ttgagacagc ccgccaggaa cagcatgagg cccaggccca ggttgcagag ctagagttgc
     4501 agctgcggtc tgagcagcaa aaagcaactg agaaagaaag ggtggcccag gagaaggacc
     4561 agctccagga gcagctccag gccctcaaag agtccttgaa ggtcaccaag ggcagccttg
     4621 aagaggagaa gcgcagggct gcagatgccc tggaagagca gcagcgttgt atctctgagc
     4681 tgaaggcaga gacccgaagc ctggtggagc agcataagcg ggaacgaaag gagctggaag
     4741 aagagagggc tgggcgcaag gggctggagg ctcgattaca gcagcttggg gaggcccatc
     4801 aggctgagac tgaagtcctg cggcgggagc tggcagaggc catggctgcc cagcacacag
     4861 ctgagagtga gtgtgagcag ctcgtcaaag aagtagctgc ctggcgtgag cggtatgagg
     4921 atagccagca agaggaggca cagtatggcg ccatgttcca ggaacagctg atgactttga
     4981 aggaggaatg tgagaaggcc cgccaggagc tgcaggaggc aaaggagaag gtggcaggca
     5041 tagaatccca cagcgagctc cagataagcc ggcagcagaa caaactagct gagctccatg
     5101 ccaacctggc cagagcactc cagcaggtcc aagagaagga agtcagggcc cagaagcttg
     5161 cagatgacct ctccactctg caggaaaaga tggctgccac cagcaaagag gtggcccgct
     5221 tggagacctt ggtgcgcaag gcaggtgagc agcaggaaac agcctcccgg gagttagtca
     5281 aggagcctgc gagggcagga gacagacagc ccgagtggct ggaagagcaa cagggacgcc
     5341 agttctgcag cacacaggca gcgctgcagg ctatggagcg ggaggcagag cagatgggca
     5401 atgagctgga acggctgcgg gccgcgctga tggagagcca ggggcagcag caggaggagc
     5461 gtgggcagca ggaaagggag gtggcgcggc tgacccagga gcggggccgt gcccaggctg
     5521 accttgccct ggagaaggcg gccagagcag agcttgagat gcggctgcag aacgccctca
     5581 acgagcagcg tgtggagttc gctaccctgc aagaggcact ggctcatgcc ctgacggaaa
     5641 aggaaggcaa ggaccaggag ttggccaagc ttcgtggtct ggaggcagcc cagataaaag
     5701 agctggagga acttcggcaa accgtgaagc aactgaagga acagctggct aagaaagaaa
     5761 aggagcacgc atctggctca ggagcccaat ctgaggctgc tggcaggaca gagccaacag
     5821 gccccaagct ggaagcactg cgggcagagg tgagcaagct ggaacagcaa tgccagaagc
     5881 agcaggagca ggctgacagc ctggaacgca gcctcgaggc tgagcgggcc tcccgggctg
     5941 agcgggacag tgctctggag actctgcagg gccagttaga ggagaaggcc caggagctag
     6001 ggcacagtca gagtgcctta gcctcggccc aacgggagtt ggctgccttc cgcaccaagg
     6061 tacaagacca cagcaaggct gaagatgagt ggaaggccca ggtggcccgg ggccggcaag
     6121 aggctgagag gaaaaatagc ctcatcagca gcttggagga ggaggtgtcc atcctgaatc
     6181 gccaggtcct ggagaaggag ggggagagca aggagttgaa gcggctggtg atggccgagt
     6241 cagagaagag ccagaagctg gaggagaggc tgcgcctgct gcaggcagag acagccagca
     6301 acagtgccag agctgcagaa cgcagctctg ctctgcggga ggaggtgcag agcctccggg
     6361 aggaggctga gaaacagcgg gtggcttcag agaacctgcg gcaggagctg acctcacagg
     6421 ctgagcgtgc ggaggagctg ggccaagaat tgaaggcgtg gcaggagaag ttcttccaga
     6481 aagagcaggc cctctccacc ctgcagctcg agcacaccag cacacaggcc ctggtgagtg
     6541 agctgctgcc agctaagcac ctctgccagc agctgcaggc cgagcaggcc gctgccgaga
     6601 aacgccaccg tgaggagctg gagcagagca agcaggccgc tgggggactg cgggcagagc
     6661 tgctgcgggc ccagcgggag cttggggagc tgattcctct gcggcagaag gtggcagagc
     6721 aggagcgaac agctcagcag ctgcgggcag agaaggccag ctatgcagag cagctgagca
     6781 tgctgaagaa ggcgcatggc ctgctggcag aggagaaccg ggggctgggt gagcgggcca
     6841 accttggccg gcagtttctg gaagtggagt tggaccaggc ccgggaaaag tatgtccaag
     6901 agttggcagc cgtacgtgct gatgctgaga cccgtctggc tgaggtgcag cgagaagcac
     6961 agagcactgc ccgggagctg gaggtgatga ctgccaagta tgagggtgcc aaggtcaagg
     7021 tcctggagga gaggcagcgg ttccaggaag agaggcagaa actcactgcc caggtggaag
     7081 aactgagtaa gaaactggct gactctgacc aagccagygg aagaactgag taagaaactg
     7141 gctgactctg accaagccag caaggtgcag cagcagaagc tgaaggctgt ccaggctcag
     7201 ggaggcgaga gccagcagga ggcccagcgc ttccaggccc agctgaatga actgcaagcc
     7261 cagttgagcc agaaggagca ggcagctgag cactataagc tgcagatgga gaaagccaaa
     7321 acacattatg atgccaagaa gcagcagaac caagagctgc aggagcagct gcggagcctg
     7381 gagcagctgc agaaggaaaa caaagagctg cgagctgaag ctgaacggct gggccatgag
     7441 ctacagcagg ctgggctgaa gaccaaggag gctgaacaga cctgccgcca ccttactgcc
     7501 caggtgcgca gcctggaggc acaggttgcc catgcagacc agcagcttcg agacctgggc
     7561 aaattccagg tggcaactga tgctttaaag agccgtgagc cccaggctaa gccccagctg
     7621 gacttgagta ttgacagcct ggatctgagc tgcgaggagg ggaccccact cagtatcacc
     7681 agcaagctgc ctcgtaccca gccagacggc accagcgtcc ctggagaacc agcctcacct
     7741 atctcccagc gcctgccccc caaggtagaa tccctggaga gtctctactt cactcccatc
     7801 cctgctcgga gtcaggcccc cctggagagc agcctggact ccctgggaga cgtcttcctg
     7861 gactcgggtc gtaagacccg ctccgctcgt cggcgcacca cgcagatcat caacatcacc
     7921 atgaccaaga agctagatgt ggaagagcca gacagcgcca actcatcgtt ctacagcacg
     7981 cggtctgctc ctgcttccca ggctagcctg cgagccacct cctctactca gtctctagct
     8041 cgcctgggtt ctcccgatta tggcaactca gccctgctca gcttgcctgg ctaccgcccc
     8101 accactcgca gttctgctcg tcgttcccag gccggggtgt ccagtggggc ccctccagga
     8161 aggaacagct tctacatggg cacttgccag gatgagcctg agcagctgga tgactggaac
     8221 cgcattgcag agctgcagca gcgcaatcga gtgtgccccc cacatctgaa gacctgctat
     8281 cccctggagt ccaggccttc cctgagcctg ggcaccatca cagatgagga gatgaaaact
     8341 ggagaccccc aagagaccct gcgccgagcc agcatgcagc caatccagat agccgagggc
     8401 actggcatca ccacccggca gcagcgcaaa cgggtctccc tagagcccca ccagggccct
     8461 ggaactcctg agtctaagaa ggccaccagc tgtttcccac gccccatgac tccccgagac
     8521 cgacatgaag ggcgcaaaca gagcactact gaggcccaga agaaagcagc tccagcttct
     8581 actaaacagg ctgaccggcg ccagtcgatg gccttcagca tcctcaacac acccaagaag
     8641 ctagggaaca gccttctgcg gcggggagcc tcaaaraagg ccctgtccaa ggcttccccc
     8701 aacacycgca gtggaacccg ccgttctccg cgcattgcca ccaccacagc cagtgccgcc
     8761 actgctgccg ccattggtgc cacccctcga gccaagggca aggcaaagca ctaaaccggt
     8821 ggatccagat ctctgcagca tatgtctaga ggatcataat cagccatacc acatttgtag
     8881 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     8941 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     9001 gcatcacaaa tttcacaaat aaagcatttt tttcactgcc gacgtcaggt ggcacttttc
     9061 ggggttgggg ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc
     9121 tgggcgtggt tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc
     9181 cgttcgcagc gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc
     9241 tgctccgccc ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac
     9301 ggaagccgca cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc
     9361 gcgccgaccg cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag
     9421 cggccgggaa ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct
     9481 gcccgcgcgg tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct
     9541 cgttgaccga atcaccgacc tctctcccca gggggatcca ccggagctta ccatggatag
     9601 atccggaaag cctgaactca ccgcgacgtc tgtcgagaag tttctgatcg aaaagttcga
     9661 cagcgtctcc gacctgatgc agctctcgga gggcgaagaa tctcgtgctt tcagcttcga
     9721 tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc gccgatggtt tctacaaaga
     9781 tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg attccggaag tgcttgacat
     9841 tggggaattc agcgagagcc tgacctattg catctcccgc cgtgcacagg gtgtcacgtt
     9901 gcaagacctg cctgaaaccg aactgcccgc tgttctgcag ccggtcgcgg aggccatgga
     9961 tgcgatcgct gcggccgatc ttagccagac gagcgggttc ggcccattcg gaccgcaagg
    10021 aatcggtcaa tacactacat ggcgtgattt catatgcgcg attgctgatc cccatgtgta
    10081 tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc gtcgcgcagg ctctcgatga
    10141 gctgatgctt tgggccgagg actgccccga agtccggcac ctcgtgcacg cggatttcgg
    10201 ctccaacaat gtcctgacgg acaatggccg cataacagcg gtcattgact ggagcgaggc
    10261 gatgttcggg gattcccaat acgaggtcgc caacatcttc ttctggaggc cgtggttggc
    10321 ttgtatggag cagcagacgc gctacttcga gcggaggcat ccggagcttg caggatcgcc
    10381 gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa ctctatcaga gcttggttga
    10441 cggcaatttc gatgatgcag cttgggcgca gggtcgatgc gacgcaatcg tccgatccgg
    10501 agccgggact gtcgggcgta cacaaatcgc ccgcagaagc gcggccgtct ggaccgatgg
    10561 ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc agcactcgtc cgagggcaaa
    10621 ggaatagagt agatgccgac cgaacaagag ctgatttcga gaacgcctca gccagcaact
    10681 cgcgcgagcc tagcaaggca aatgcgagag aacggcctta cgcttggtgg cacagttctc
    10741 gtccacagtt cgctaagctc gctcggctgg gtcgcgggag ggccggtcgc agtgattcag
    10801 gcccttctgg attgtgttgg tccccagggc acgattgtca tgcccacgca ctcgggtgat
    10861 ctgactgatc ccgcagattg gagatcgccg cccgtgcctg ccgattgggt gcagatctct
    10921 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
    10981 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
    11041 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    11101 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    11161 tctatggctt ctgaggcgga aagaaccagc tggggctcga tgagggagct gcggtggagg
    11221 aggtggagag aaggccgcac ccttctgggc agggggaggg gagtgccgca atacctttat
    11281 gggagttctc tgctgcctcc cgtcttgtaa ggaccgccct gggcctggaa gaagccctcc
    11341 ctcctttcct cctcgcgtga tctcgtcatc gcctccatgt cgagtcgctt ctcgattatg
    11401 ggcgggattc ttttgcctag gcttaagggg ctaacttggt ccctgggcgt tgccctgcag
    11461 gggagtgagc agctgtaaga tttgaggggc gactccgatt agtttatctt cccacggact
    11521 agagttggtg tcgaggttat tgtaataagg gtggggtagg gaaatggagc ttagtcattc
    11581 acctggggct gattttatgc aacgagactg cggattatca ctaatcattt ttggagcatt
    11641 tttctagaga cagacataaa gcatgatcac ctgagtttta taccatttga gacccttgct
    11701 gcaccaccaa agtgtagcat caggttaaat cttaatagaa aaattttagc ttttgcttga
    11761 gaaaccagtg cttccctccc tcaccctctc tccccaggct ctctacccct ttgcatccct
    11821 accaggcatc ttagcaactc tcactcatac ttgatcccat tttccatttg ttgtacttgc
    11881 tcctccagta ttcagacata gcactagctt tctccctctc ttgatcttgg gtagcctggt
    11941 gtctcgcgaa accagacaga ttggttccac cacaaattaa ggcttgagct ggggcttgac
    12001 tcttacccag cagtgctttt attcctccct agttcacgtt cttaaatgtt tatcttgatt
    12061 ttcattttat cctttttcct tagctgggat tctgtccctg accgtcttca cagtccaggt
    12121 gatcttgact actgctttac agagaattgg atctgaggtt aggcaacatc tccctttttc
    12181 ttcctctaaa tacctctcat ttctgttctt accagttagt aactgatctc agatgcctgt
    12241 gtgatagctg gtacccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
    12301 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
    12361 acgagccggg agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
    12421 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
    12481 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
    12541 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
    12601 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    12661 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    12721 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    12781 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    12841 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    12901 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    12961 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    13021 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    13081 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    13141 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    13201 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    13261 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    13321 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    13381 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    13441 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    13501 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    13561 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    13621 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    13681 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    13741 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    13801 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    13861 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    13921 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    13981 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    14041 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    14101 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    14161 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    14221 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    14281 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    14341 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    14401 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac
//
