LOCUS       sequence-22531-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Takahashi Y, Kahyo T, Toh-E A, Yasuda H, Kikuchi Y
  TITLE     Yeast Ull1/Siz1 is a novel SUMO1/Smt3 ligase for septin components 
            and functions as an adaptor between conjugating enzyme and 
            substrates.
  JOURNAL   J Biol Chem. 2001 Dec 28. 276(52):48973-7.
  PUBMED    11577116
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Dec 28. 276(52):48973-7."
COMMENT     Sequence Label: pT-35
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             6..23
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             24..317
                     /codon_start=1
                     /gene="S. cerevisiae SMT3 (truncated)"
                     /product="cleavable ubiquitin-like protein tag"
                     /label=SUMO
                     /translation="MSDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPL
                     RRLMEAFAKRQGKEMDSLRFLYDGIRIQADQTPEDLDMEDNDIIEAHREQIGG"
     primer_bind     complement(397..419)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(564..582)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        650..754
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cggatctggt tccgcgtgga tccatgtcgg actcagaagt caatcaagaa gctaagccag
       61 aggtcaagcc agaagtcaag cctgagactc acatcaattt aaaggtgtcc gatggatctt
      121 cagagatctt cttcaagatc aaaaagacca ctcctttaag aaggctgatg gaagcgttcg
      181 ctaaaagaca gggtaaggaa atggactcct taagattctt gtacgacggt attagaattc
      241 aagctgatca gacccctgaa gatttggaca tggaggataa cgatattatt gaggctcaca
      301 gagaacagat tggtggttag tcgactcgag ctcaagctta attcatcgtg actgactgac
      361 gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
      421 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
      481 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
      541 gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      601 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      661 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      721 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attca
//
