LOCUS       sequence-225311-       14455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mCherry-NuMA(5A-3) from Rosa 26 locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14455)
  AUTHORS   Okumura M, Natsume T, Kanemaki MT, Kiyomitsu T
  TITLE     Dynein-Dynactin-NuMA clusters generate cortical spindle-pulling 
            forces as a multi-arm ensemble.
  JOURNAL   Elife. 2018 May 31;7. pii: 36559. doi: 10.7554/eLife.36559.
  PUBMED    29848445
REFERENCE   2  (bases 1 to 14455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2018-05-31"; volume: "7. pii: 36559. doi"; pages: " 
            10.7554/eLife.36559"
COMMENT     Sequence Label: pTK750
FEATURES             Location/Qualifiers
     source          1..14455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1165..1213
                     /note="synthetic polyadenylation signal"
     misc_feature    1227..1318
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     1267..1286
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        1322..1689
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    1330..1348
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1366..1384
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1402..1420
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1438..1456
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1474..1492
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1510..1528
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1546..1564
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             1710..2417
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1855..1873)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2123..2142
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    8957..9038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8994..9013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9060..9570
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9489..9507
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9607..10626
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     CDS             9607..10626
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="KPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRGYV
                     LRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLPET
                     ELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQTV
                     MDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMFGD
                     SQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDGNF
                     DDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAKE"
     primer_bind     complement(10936..10953)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10942..11166
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(12267..12287)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(12267..12285)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12306..12322)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12306..12322)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12319..12341)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12330..12346
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12354..12360,12361..12378,12379..12384))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12399..12420
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12537..12554)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12708..13296)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12788..12807)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13467..14258,14259..14327))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14090..14109
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14328..14432)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgg
      661 aactctggtg ggaggggagg gtcagtgaaa ttggctctgg cgcgggcgtc ctcccaccct
      721 ccccttcctt cgggggagtc ggtttacccg ccgcctgctt gtcttcgaca cctgattggc
      781 tgtcgaagct gtgggaccgg gcccttgcta ctggctcgag tctcacatga gcgaaaccac
      841 tgcgcggggc gcgggggtgg cggggaggcg ggcgttggta cggtcctccc cgaggccgag
      901 cgccgcagtg tctggccccg cgcccctgcg caacgtggca ggaagcgcgc gctggaggcg
      961 ggggcgggct gccggccgag acttctggat ggcggcggcc gcggctccgc cccgggttcc
     1021 caccgcctga agggcgagac aagcccgacc tgctacaggc actcgtgggg gtgggggagg
     1081 agcgggggtc ggtccggctg gtttgtgggt gggaggcgct tgttctccaa aaaccggcgc
     1141 gcgttgtgga attatcacct cgagaataaa atatctttat tttcattaca tctgtgtgtt
     1201 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
     1261 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctct
     1321 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
     1381 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
     1441 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
     1501 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
     1561 gagaacgtat aagctttgct tatgtaaacc agggcgccta taaaagagtg ctgatttttt
     1621 gagtaaactt caattccaca acacttttgt cttataccaa ctttccgtac cacttcctac
     1681 cctcgtaaag aattcgcggc cgcacgcgta tggtgagcaa gggcgaggag gataacatgg
     1741 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
     1801 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
     1861 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
     1921 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
     1981 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
     2041 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
     2101 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
     2161 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
     2221 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
     2281 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
     2341 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
     2401 tggacgagct gtacaagtcc ggaggggatc ggtggagctc caccggagga ggtggatctg
     2461 tcgacatgac actccacgcc acccgggggg ctgcactcct ctcttgggtg aacagtctac
     2521 acgtggctga ccctgtggag gctgtgctgc agctccagga ctgcagcatc ttcatcaaga
     2581 tcattgacag aatccatggc actgaagagg gacagcaaat cttgaagcag ccggtgtcag
     2641 agagactgga ctttgtgtgc agttttctgc agaaaaatcg aaaacatccc tcttccccag
     2701 aatgcctggt atctgcacag aaggtgctag agggatcaga gctggaactg gcgaagatga
     2761 ccatgctgct cttataccac tctaccatga gctccaaaag tcccagggac tgggaacagt
     2821 ttgaatataa aattcaggct gagttggctg tcattcttaa atttgtgctg gaccatgagg
     2881 acgggctaaa ccttaatgag gacctagaga acttcctaca gaaagctcct gtgccttcta
     2941 cctgttctag cacattccct gaagagctct ccccacctag ccaccaggcc aagagggaga
     3001 ttcgcttcct agagctacag aaggttgcct cctcttccag tgggaacaac tttctctcag
     3061 gttctccagc ttctcccatg ggtgatatcc tgcagacccc acagttccag atgagacggc
     3121 tgaagaagca gcttgctgat gagagaagta atagggatga gctggagctg gagctagctg
     3181 agaaccgcaa gctcctcacc gagaaggatg cacagatagc catgatgcag cagcgcattg
     3241 accgcctagc cctgctgaat gagaagcagg cggccagccc actggagccc aaggagcttg
     3301 aggagctgcg tgacaagaat gagagcctta ccatgcggct gcatgaaacc ctgaagcagt
     3361 gccaggacct gaagacagag aagagccaga tggatcgcaa aatcaaccag ctttcggagg
     3421 agaatggaga cctttccttt aagctgcggg agtttgccag tcatctgcag cagctacagg
     3481 atgccctcaa tgagctgacg gaggagcaca gcaaggccac tcaggagtgg ctagagaagc
     3541 aggcccagct ggagaaggag ctcagcgcag ccctgcagga caagaaatgc cttgaagaga
     3601 agaacgaaat ccttcaggga aaactttcac agctggaaga acacttgtcc cagctgcagg
     3661 ataacccacc ccaggagaag ggcgaggtgc tgggtgatgt cttgcagctg gaaaccttga
     3721 agcaagaggc agccactctt gctgcaaaca acacacagct ccaagccagg gtagagatgc
     3781 tggagactga gcgaggccag caggaagcca agctgcttgc tgagcggggc cacttcgaag
     3841 aagaaaagca gcagctgtct agcctgatca ctgacctgca gagctccatc tccaacctca
     3901 gccaggccaa ggaagagctg gagcaggcct cccaggctca tggggcccgg ttgactgccc
     3961 aggtggcctc tctgacctct gagctcacca cactcaatgc caccatccag caacaggatc
     4021 aagaactggc tggcctgaag cagcaggcca aagagaagca ggcccagcta gcacagaccc
     4081 tccaacagca agaacaggcc tcccagggcc tccgccacca ggtggagcag ctaagcagta
     4141 gcctgaagca gaaggagcag cagttgaagg aggtagcgga gaagcaggag gcaactaggc
     4201 aggaccatgc ccagcaactg gccactgctg cagaggagcg agaggcctcc ttaagggagc
     4261 gggatgcggc tctcaagcag ctggaggcac tggagaagga gaaggctgcc aagctggaga
     4321 ttctgcagca gcaacttcag gtggctaatg aagcccggga cagtgcccag acctcagtga
     4381 cacaggccca gcgggagaag gcagagctga gccggaaggt ggaggaactc caggcctgtg
     4441 ttgagacagc ccgccaggaa cagcatgagg cccaggccca ggttgcagag ctagagttgc
     4501 agctgcggtc tgagcagcaa aaagcaactg agaaagaaag ggtggcccag gagaaggacc
     4561 agctccagga gcagctccag gccctcaaag agtccttgaa ggtcaccaag ggcagccttg
     4621 aagaggagaa gcgcagggct gcagatgccc tggaagagca gcagcgttgt atctctgagc
     4681 tgaaggcaga gacccgaagc ctggtggagc agcataagcg ggaacgaaag gagctggaag
     4741 aagagagggc tgggcgcaag gggctggagg ctcgattaca gcagcttggg gaggcccatc
     4801 aggctgagac tgaagtcctg cggcgggagc tggcagaggc catggctgcc cagcacacag
     4861 ctgagagtga gtgtgagcag ctcgtcaaag aagtagctgc ctggcgtgag cggtatgagg
     4921 atagccagca agaggaggca cagtatggcg ccatgttcca ggaacagctg atgactttga
     4981 aggaggaatg tgagaaggcc cgccaggagc tgcaggaggc aaaggagaag gtggcaggca
     5041 tagaatccca cagcgagctc cagataagcc ggcagcagaa caaactagct gagctccatg
     5101 ccaacctggc cagagcactc cagcaggtcc aagagaagga agtcagggcc cagaagcttg
     5161 cagatgacct ctccactctg caggaaaaga tggctgccac cagcaaagag gtggcccgct
     5221 tggagacctt ggtgcgcaag gcaggtgagc agcaggaaac agcctcccgg gagttagtca
     5281 aggagcctgc gagggcagga gacagacagc ccgagtggct ggaagagcaa cagggacgcc
     5341 agttctgcag cacacaggca gcgctgcagg ctatggagcg ggaggcagag cagatgggca
     5401 atgagctgga acggctgcgg gccgcgctga tggagagcca ggggcagcag caggaggagc
     5461 gtgggcagca ggaaagggag gtggcgcggc tgacccagga gcggggccgt gcccaggctg
     5521 accttgccct ggagaaggcg gccagagcag agcttgagat gcggctgcag aacgccctca
     5581 acgagcagcg tgtggagttc gctaccctgc aagaggcact ggctcatgcc ctgacggaaa
     5641 aggaaggcaa ggaccaggag ttggccaagc ttcgtggtct ggaggcagcc cagataaaag
     5701 agctggagga acttcggcaa accgtgaagc aactgaagga acagctggct aagaaagaaa
     5761 aggagcacgc atctggctca ggagcccaat ctgaggctgc tggcaggaca gagccaacag
     5821 gccccaagct ggaagcactg cgggcagagg tgagcaagct ggaacagcaa tgccagaagc
     5881 agcaggagca ggctgacagc ctggaacgca gcctcgaggc tgagcgggcc tcccgggctg
     5941 agcgggacag tgctctggag actctgcagg gccagttaga ggagaaggcc caggagctag
     6001 ggcacagtca gagtgcctta gcctcggccc aacgggagtt ggctgccttc cgcaccaagg
     6061 tacaagacca cagcaaggct gaagatgagt ggaaggccca ggtggcccgg ggccggcaag
     6121 aggctgagag gaaaaatagc ctcatcagca gcttggagga ggaggtgtcc atcctgaatc
     6181 gccaggtcct ggagaaggag ggggagagca aggagttgaa gcggctggtg atggccgagt
     6241 cagagaagag ccagaagctg gaggagaggc tgcgcctgct gcaggcagag acagccagca
     6301 acagtgccag agctgcagaa cgcagctctg ctctgcggga ggaggtgcag agcctccggg
     6361 aggaggctga gaaacagcgg gtggcttcag agaacctgcg gcaggagctg acctcacagg
     6421 ctgagcgtgc ggaggagctg ggccaagaat tgaaggcgtg gcaggagaag ttcttccaga
     6481 aagagcaggc cctctccacc ctgcagctcg agcacaccag cacacaggcc ctggtgagtg
     6541 agctgctgcc agctaagcac ctctgccagc agctgcaggc cgagcaggcc gctgccgaga
     6601 aacgccaccg tgaggagctg gagcagagca agcaggccgc tgggggactg cgggcagagc
     6661 tgctgcgggc ccagcgggag cttggggagc tgattcctct gcggcagaag gtggcagagc
     6721 aggagcgaac agctcagcag ctgcgggcag agaaggccag ctatgcagag cagctgagca
     6781 tgctgaagaa ggcgcatggc ctgctggcag aggagaaccg ggggctgggt gagcgggcca
     6841 accttggccg gcagtttctg gaagtggagt tggaccaggc ccgggaaaag tatgtccaag
     6901 agttggcagc cgtacgtgct gatgctgaga cccgtctggc tgaggtgcag cgagaagcac
     6961 agagcactgc ccgggagctg gaggtgatga ctgccaagta tgagggtgcc aaggtcaagg
     7021 tcctggagga gaggcagcgg ttccaggaag agaggcagaa actcactgcc caggkggaag
     7081 agttccagga agagaggcag aaactcactg cccaggtgga agaactgagt aagaaactgg
     7141 ctgactctga ccaagccagc aaggtgcagc agcagaagct gaaggctgtc caggctcagg
     7201 gaggcgagag ccagcaggag gcccagcgct tccaggccca gctgaatgaa ctgcaagccc
     7261 agttgagcca gaaggagcag gcagctgagc actataagct gcagatggag aaagccaaaa
     7321 cacattatga tgccaagaag cagcagaacc aagagctgca ggagcagctg cggagcctgg
     7381 agcagctgca gaaggaaaac aaagagctgc gagctgaagc tgaacggctg ggccatgagc
     7441 tacagcaggc tgggctgaag accaaggagg ctgaacagac ctgccgccac cttactgccc
     7501 aggtgcgcag cctggaggca caggttgccc atgcagacca gcagcttcga gacctgggca
     7561 aattccaggt ggcaactgat gctttaaaga gccgtgagcc ccaggctaag ccccagctgg
     7621 acttgagtat tgacagcctg gatctgagct gcgaggaggg gaccccactc agtatcacca
     7681 gcaagctgcc tcgtacccag ccagacggca ccagcgtccc tggagaacca gcctcaccta
     7741 tctcccagcg cctgcccccc aaggtagaat ccctggagag tgccgccgcc gctgccatcc
     7801 ctgctcggag tcaggccccc ctggagagca gcctggactc cctgggagac gtcttcctgg
     7861 actcgggtcg taagacccgc tccgctcgtc ggcgcaccac gcagatcatc aacatcacca
     7921 tgaccaagaa gctagatgtg gaagagccag acagcgccaa ctcatcgttc tacagcacgc
     7981 ggtctgctcc tgcttcccag gctagcctgc gagccacctc ctctactcag tctctagctc
     8041 gcctgggttc tcccgattat ggcaactcag ccctgctcag cttgcctggc taccgcccca
     8101 ccactcgcag ttctgctcgt cgttcccagg ccggggtgtc cagtggggcc cctccaggaa
     8161 ggaacagctt ctacatgggc acttgccagg atgagcctga gcagctggat gactggaacc
     8221 gcattgcaga gctgcagcag cgcaatcgag tgtgcccccc acatctgaag acctgctatc
     8281 ccctggagtc caggccttcc ctgagcctgg gcaccatcac agatgaggag atgaaaactg
     8341 gagaccccca agagaccctg cgccgagcca gcatgcagcc aatccagata gccgagggca
     8401 ctggcatcac cacccggcag cagcgcaaac gggtctccct agagccccac cagggccctg
     8461 gaactcctga gtctaagaag gccaccagct gtttcccacg ccccatgact ccccgagacc
     8521 gacatgaagg gcgcaaacag agcactactg aggcccagaa gaaagcagct ccagcttcta
     8581 ctaaacaggc tgaccggcgc cagtcgatgg ccttcagcat cctcaacaca cccaagaagc
     8641 tagggaacag ccttctgcgg cggggagcct caaaraaggc cctgtccaag gcttccccca
     8701 acacycgcag tggaacccgc cgttctccgc gcattgccac caccacagcc agtgccgcca
     8761 ctgctgccgc cattggtgcc acccctcgag ccaagggcaa ggcaaagcac taaaccggtg
     8821 gatccagatc tctgcagcat atgtctagag gatcataatc agccatacca catttgtaga
     8881 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
     8941 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     9001 catcacaaat ttcacaaata aagcattttt ttcactgccg acgtcaggtg gcacttttcg
     9061 gggttggggt tgcgcctttt ccaaggcagc cctgggtttg cgcagggacg cggctgctct
     9121 gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt ctcgcacatt cttcacgtcc
     9181 gttcgcagcg tcacccggat cttcgccgct acccttgtgg gccccccggc gacgcttcct
     9241 gctccgcccc taagtcggga aggttccttg cggttcgcgg cgtgccggac gtgacaaacg
     9301 gaagccgcac gtctcactag taccctcgca gacggacagc gccagggagc aatggcagcg
     9361 cgccgaccgc gatgggctgt ggccaatagc ggctgctcag cagggcgcgc cgagagcagc
     9421 ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg tagtgtgggc cctgttcctg
     9481 cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca cgtcggcagt cggctccctc
     9541 gttgaccgaa tcaccgacct ctctccccag ggggatccac cggagcttac catggataga
     9601 tccggaaagc ctgaactcac cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac
     9661 agcgtctccg acctgatgca gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat
     9721 gtaggagggc gtggatatgt cctgcgggta aatagctgcg ccgatggttt ctacaaagat
     9781 cgttatgttt atcggcactt tgcatcggcc gcgctcccga ttccggaagt gcttgacatt
     9841 ggggaattca gcgagagcct gacctattgc atctcccgcc gtgcacaggg tgtcacgttg
     9901 caagacctgc ctgaaaccga actgcccgct gttctgcagc cggtcgcgga ggccatggat
     9961 gcgatcgctg cggccgatct tagccagacg agcgggttcg gcccattcgg accgcaagga
    10021 atcggtcaat acactacatg gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat
    10081 cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag
    10141 ctgatgcttt gggccgagga ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc
    10201 tccaacaatg tcctgacgga caatggccgc ataacagcgg tcattgactg gagcgaggcg
    10261 atgttcgggg attcccaata cgaggtcgcc aacatcttct tctggaggcc gtggttggct
    10321 tgtatggagc agcagacgcg ctacttcgag cggaggcatc cggagcttgc aggatcgccg
    10381 cggctccggg cgtatatgct ccgcattggt cttgaccaac tctatcagag cttggttgac
    10441 ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga
    10501 gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc
    10561 tgtgtagaag tactcgccga tagtggaaac cgacgcccca gcactcgtcc gagggcaaag
    10621 gaatagagta gatgccgacc gaacaagagc tgatttcgag aacgcctcag ccagcaactc
    10681 gcgcgagcct agcaaggcaa atgcgagaga acggccttac gcttggtggc acagttctcg
    10741 tccacagttc gctaagctcg ctcggctggg tcgcgggagg gccggtcgca gtgattcagg
    10801 cccttctgga ttgtgttggt ccccagggca cgattgtcat gcccacgcac tcgggtgatc
    10861 tgactgatcc cgcagattgg agatcgccgc ccgtgcctgc cgattgggtg cagatctcta
    10921 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
    10981 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
    11041 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
    11101 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
    11161 ctatggcttc tgaggcggaa agaaccagct ggggctcgat gagggagctg cggtggagga
    11221 ggtggagaga aggccgcacc cttctgggca gggggagggg agtgccgcaa tacctttatg
    11281 ggagttctct gctgcctccc gtcttgtaag gaccgccctg ggcctggaag aagccctccc
    11341 tcctttcctc ctcgcgtgat ctcgtcatcg cctccatgtc gagtcgcttc tcgattatgg
    11401 gcgggattct tttgcctagg cttaaggggc taacttggtc cctgggcgtt gccctgcagg
    11461 ggagtgagca gctgtaagat ttgaggggcg actccgatta gtttatcttc ccacggacta
    11521 gagttggtgt cgaggttatt gtaataaggg tggggtaggg aaatggagct tagtcattca
    11581 cctggggctg attttatgca acgagactgc ggattatcac taatcatttt tggagcattt
    11641 ttctagagac agacataaag catgatcacc tgagttttat accatttgag acccttgctg
    11701 caccaccaaa gtgtagcatc aggttaaatc ttaatagaaa aattttagct tttgcttgag
    11761 aaaccagtgc ttccctccct caccctctct ccccaggctc tctacccctt tgcatcccta
    11821 ccaggcatct tagcaactct cactcatact tgatcccatt ttccatttgt tgtacttgct
    11881 cctccagtat tcagacatag cactagcttt ctccctctct tgatcttggg tagcctggtg
    11941 tctcgcgaaa ccagacagat tggttccacc acaaattaag gcttgagctg gggcttgact
    12001 cttacccagc agtgctttta ttcctcccta gttcacgttc ttaaatgttt atcttgattt
    12061 tcattttatc ctttttcctt agctgggatt ctgtccctga ccgtcttcac agtccaggtg
    12121 atcttgacta ctgctttaca gagaattgga tctgaggtta ggcaacatct ccctttttct
    12181 tcctctaaat acctctcatt tctgttctta ccagttagta actgatctca gatgcctgtg
    12241 tgatagctgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
    12301 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
    12361 cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
    12421 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
    12481 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
    12541 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
    12601 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
    12661 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
    12721 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
    12781 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
    12841 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
    12901 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
    12961 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
    13021 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    13081 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    13141 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    13201 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    13261 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    13321 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    13381 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    13441 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    13501 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    13561 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    13621 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    13681 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    13741 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    13801 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    13861 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    13921 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    13981 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    14041 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    14101 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    14161 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    14221 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    14281 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    14341 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    14401 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
