LOCUS       sequence-225316-       11496 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acts as a reporter for APOBEC-medated single-base editing. mCherry 
            acts as a transfection control and eGFP as a reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11496)
  AUTHORS   Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris 
            RS
  TITLE     A panel of eGFP reporters for single base editing by APOBEC-Cas9 
            editosome complexes.
  JOURNAL   Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
  PUBMED    30679582
REFERENCE   2  (bases 1 to 11496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-01-24"; volume: "9(1):497. doi"; pages: " 
            10.1038/s41598-018-36739-9"
COMMENT     Sequence Label: eGFP L138 Reporter
FEATURES             Location/Qualifiers
     source          1..11496
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             377..634
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1568..1685
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2528..2761
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2946..2990
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             3284..3310
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     enhancer        3627..3930
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3931..4134
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4085..4105
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4131..4155
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             4171..4878
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4316..4334)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4584..4603
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             4903..4956
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(4957..4959,4960..4962,4963..5676)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNISGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5002..5023)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5263..5282)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5610..5631
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5748..6381
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8020..8037)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8191..8779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8271..8290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8950..9741,9742..9810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9573..9592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9811..9915)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9983..10001
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10039..10061)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     10161..10180
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 tggaagggct aatttggtcc caaaaaagac aagagatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
      121 tgacctttgg atggtgcttc aagttagtac cagttgaacc agagcaagta gaagaggcca
      181 atgaaggaga gaacaacagc ttgttacacc ctatgagcca gcatgggatg gaggacccgg
      241 agggagaagt attagtgtgg aagtttgaca gcctcctagc atttcgtcac atggcccgag
      301 agctgcatcc ggagtactac aaagactgct gacatcgagc tttctacaag ggactttccg
      361 ctggggactt tccagggagg tgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 gctacatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgctca aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagtaaa gccagaggag atctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcggta ttaagcgggg gagaattaga taaatgggaa
      841 aaaattcggt taaggccagg gggaaagaaa caatataaac taaaacatat agtatgggca
      901 agcagggagc tagaacgatt cgcagttaat cctggccttt tagagacatc agaaggctgt
      961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1021 ttatataata caatagcagt cctctattgt gtgcatcaaa ggatagatgt aaaagacacc
     1081 aaggaagcct tagataagat agaggaagag caaaacaaaa gtaagaaaaa ggcacagcaa
     1141 gcagcagctg acacaggaaa caacagccag gtcagccaaa attaccctat agtgcagaac
     1201 ctccaggggc aaatggtaca tcaggccata tcacctagaa ctttaaatgc atgggtaaaa
     1261 gtagtagaag agaaggcttt cagcccagaa gtaataccca tgttttcagc attatcagaa
     1321 ggagccaccc cacaagattt ccagtaaaaa cagtacatac agacaatggc agcaatttca
     1381 ccagtactac agttaaggcc gcctgttggt gggcggggat caagcaggaa tttggcattc
     1441 cctacaatcc ccaaagtcaa ggagtaatag aatctatgaa taaagaatta aagaaaatta
     1501 taggacaggt aagagatcag gctgaacatc ttaagacagc agtacaaatg gcagtattca
     1561 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     1621 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     1681 attttcgggt ttattacagg gacagcagag atccagtttg gaaaggacca gcaaagctcc
     1741 tctggaaagg tgaaggggca gtagtaatac aagataatag tgacataaaa gtagtgccaa
     1801 gaagaaaagc aaagatcatc agggattatg gaaaacagat ggcaggtgat gattgtgtgg
     1861 caagtagaca ggatgaggat taacacatgg aaaagattag taaaacacca tatgggattg
     1921 gaagccataa taagaattct gcaacaactg ctgtttatcc atttcagaat tgggtgtcga
     1981 catagcagaa taggcgttac tcgacagagg agagcaagaa atggagccag tagatcctag
     2041 actagagccc tggaagcatc caggaagtca gcctaaaact gcttgtacca attgctattg
     2101 taaaaagtgt tgctttcatt gccaagtttg tttcatgaca aaagccttag gcatctccta
     2161 tggcaggaag aagcggagac agcgacgaag agctcatcag aacagtcaga ctcatcaagc
     2221 ttctctatca aagcagtaag tagtacatgg gcgcgcccat gtggcaggaa gtaggaaaag
     2281 caatgtatgc ccctcccatc agtggacaaa ttagatgttc atcaaatatt actgggctgc
     2341 tattaacaag agatggtggt aataacaaca atgggtccga gatcttcaga cctggaggag
     2401 gcgatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     2461 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     2521 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     2581 cgtcaatgac gctgacggta caggccagac aattattgtc tgatatagtg cagcagcaga
     2641 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     2701 tcaaacagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     2761 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     2821 gttggagtaa taaatctctg gaacagattt ggaataacat gacctggatg gagtgggaca
     2881 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     2941 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     3001 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     3061 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     3121 caccattatc gtttcagacc cacctcccaa tcccgagggg acccgacagg cccgaaggaa
     3181 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatcct
     3241 tagcacttat ctgggacgat ctgcggagcc tgtgcctctt cagctaccac cgcttgagag
     3301 acttactctt gattgtaacg aggattgtgg aacttctggg acgcaggggg tgggaagccc
     3361 tcaaatattg gtggaatctc ctacaatatt ggagtcagga gctaaagaat agtgctgtta
     3421 gcttgctcaa tgccacagcc atagcagtag ctgaggggac agatagggtt atagaagtag
     3481 tacaaggagc ttgtagagct attcgccaca tacctagaag aataagacag ggcttggaaa
     3541 ggattttgct ataagatggg tggcgcggcc gcttaatagt aatcaattac ggggtcatta
     3601 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     3661 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     3721 ccaattggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     3781 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     3841 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     3901 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     3961 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4021 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     4081 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     4141 gtgaaccgtc agatccagct ggctaccggt atggtgagca agggcgagga ggataacatg
     4201 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     4261 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     4321 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     4381 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     4441 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     4501 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     4561 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     4621 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     4681 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     4741 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     4801 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     4861 atggacgagc tgtacaagcc cggtgcagat ctcgagtaca aggagggcag aggaagtctt
     4921 ctaacatgcg gtgacgtgga ggagaatccc ggccctatgg tgagcaaggg cgaggagctg
     4981 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     5041 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     5101 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     5161 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     5221 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     5281 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     5341 atcgacttca aggaggacgg caacatttca gggcacaagc tggagtacaa ctacaacagc
     5401 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     5461 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     5521 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
     5581 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     5641 gggatcactc tcggcatgga cgagctgtac aagtaaggta cctttaagac caatgactta
     5701 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     5761 tcactcccaa agaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     5821 ccctgattgg cagaactaca caccagggcc aggggtcaga tatccactga cctttggatg
     5881 gtgctacaag ctagtaccag ttgagccaga taaggtagaa gaggccaata aaggagagaa
     5941 caccagcttg ttacaccctg tgagcctgca tggaatggat gaccctgaga gagaagtgtt
     6001 agagtggagg tttgacagcc gcctagcatt tcatcacgtg gcccgagagc tgcatccgga
     6061 gtacttcaag aactgctgac atcgagcttg ctacaaggga ctttccgctg gggactttcc
     6121 agggaggcgt ggcctgggcg ggactgggga gtggcgagcc ctcagatgct gcatataagc
     6181 agctgctttt tgcctgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6241 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6301 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6361 cagtgtggaa aatctctagc acccaggagg tagaggttgc agtgagccaa gatcgcgcca
     6421 ctgcattcca gcctgggcaa gaaaacaaga ctgtctaaaa taataataat aagttaaggg
     6481 tattaaatat atttatacat ggaggtcata aaaatatata tatttgggct gggcgcagtg
     6541 gctcacacct gcgcccggcc ctttgggagg ccgaggcagg tggatcacct gagtttggga
     6601 gttccagacc agcctgacca acatggagaa accccttctc tgtgtatttt tagtagattt
     6661 tattttatgt gtattttatt cacaggtatt tctggaaaac tgaaactgtt tttcctctac
     6721 tctgatacca caagaatcat cagcacagag gaagacttct gtgatcaaat gtggtgggag
     6781 agggaggttt tcaccagcac atgagcagtc agttctgccg cagactcggc gggtgtcctt
     6841 cggttcagtt ccaacaccgc ctgcctggag agaggtcaga ccacagggtg agggctcagt
     6901 ccccaagaca taaacaccca agacataaac acccaacagg tccaccccgc ctgctgccca
     6961 ggcagagccg attcaccaag acgggaatta ggatagagaa agagtaagtc acacagagcc
     7021 ggctgtgcgg gagaacggag ttctattatg actcaaatca gtctccccaa gcattcgggg
     7081 atcagagttt ttaaggataa cttagtgtgt agggggccag tgagttggag atgaaagcgt
     7141 agggagtcga aggtgtcctt ttgcgccgag tcagttcctg ggtgggggcc acaagatcgg
     7201 atgagccagt ttatcaatcc gggggtgcca gctgatccat ggagtgcagg gtctgcaaaa
     7261 tatctcaagc actgattgat cttaggtttt acaatagtga tgttacccca ggaacaattt
     7321 ggggaaggtc agaatcttgt agcctgtagc tgcatgactc ctaaaccata atttcttttt
     7381 tgtttttttt tttttatttt tgagacaggg tctcactctg tcacctaggc tggagtgcag
     7441 tggtgcaatc acagctcact gcagcctcaa cgtcgtaagc tcaagcgatc ctcccacctc
     7501 agcctgcctg gtagctgaga ctacaagcga cgccccagtt aatttttgta tttttggtag
     7561 aggcagcgtt ttgccgtgtg gccctggctg gtctcgaact cctgggctca agtgatccag
     7621 cctcagcctc ccaaagtgct gggacaaccg ggcccagtca ctgcacctgg ccctaaacca
     7681 taatttctaa tcttttggct aatttgttag tcctacaaag gcagtctagt ccccagcaaa
     7741 aagggggttt gtttcgggaa agggctgtta ctgtctttgt ttcaaactat aaactaagtt
     7801 cctcctaaac ttagttcggc ctacacccag gaatgaacaa ggagagcttg gaggttagaa
     7861 gcacgatgga attggttagg tcagatctct ttcactgtct gagttataat tttgcaatgg
     7921 tggttcaaag actgcccgct tctgacacca gtcgctgcat taatgaatcg gccaacgcgc
     7981 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     8041 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     8101 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     8161 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     8221 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     8281 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     8341 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     8401 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     8461 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     8521 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     8581 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     8641 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     8701 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     8761 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     8821 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     8881 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     8941 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     9001 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     9061 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     9121 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     9181 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     9241 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     9301 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     9361 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     9421 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     9481 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     9541 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     9601 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     9661 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     9721 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     9781 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     9841 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     9901 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     9961 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt
    10021 cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct
    10081 gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg
    10141 tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg
    10201 gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc attcgccatt
    10261 caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccaggg
    10321 gaggcagaga ttgcagtaag ctgagatcgc agcactgcac tccagcctgg gcgacagagt
    10381 aagactctgt ctcaaaaata aaataaataa atcaatcaga tattccaatc ttttccttta
    10441 tttatttatt tattttctat tttggaaaca cagtccttcc ttattccaga attacacata
    10501 tattctattt ttctttatat gctccagttt tttttagacc ttcacctgaa atgtgtgtat
    10561 acaaaatcta ggccagtcca gcagagccta aaggtaaaaa ataaaataat aaaaaataaa
    10621 taaaatctag ctcactcctt cacatcaaaa tggagataca gctgttagca ttaaatacca
    10681 aataacccat cttgtcctca ataattttaa gcgcctctct ccaccacatc taactcctgt
    10741 caaaggcatg tgccccttcc gggcgctctg ctgtgctgcc aaccaactgg catgtggact
    10801 ctgcagggtc cctaactgcc aagccccaca gtgtgccctg aggctgcccc ttccttctag
    10861 cggctgcccc cactcggctt tgctttccct agtttcagtt acttgcgttc agccaaggtc
    10921 tgaaactagg tgcgcacaga gcggtaagac tgcgagagaa agagaccagc tttacagggg
    10981 gtttatcaca gtgcaccctg acagtcgtca gcctcacagg gggtttatca cattgcaccc
    11041 tgacagtcgt cagcctcaca gggggtttat cacagtgcac ccttacaatc attccatttg
    11101 attcacaatt tttttagtct ctactgtgcc taacttgtaa gttaaatttg atcagaggtg
    11161 tgttcccaga ggggaaaaca gtatatacag ggttcagtac tatcgcattt caggcctcca
    11221 cctgggtctt ggaatgtgtc ccccgagggg tgatgactac ctcagttgga tctccacagg
    11281 tcacagtgac acaagataac caagacacct cccaaggcta ccacaatggg ccgccctcca
    11341 cgtgcacatg gccggaggaa ctgccatgtc ggaggtgcaa gcacacctgc gcatcagagt
    11401 ccttggtgtg gagggaggga ccagcgcagc ttccagccat ccacctgatg aacagaacct
    11461 agggaaagcc ccagttctac ttacaccagg aaaggc
//
