LOCUS       sequence-22532-e         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Takahashi Y, Kahyo T, Toh-E A, Yasuda H, Kikuchi Y
  TITLE     Yeast Ull1/Siz1 is a novel SUMO1/Smt3 ligase for septin components 
            and functions as an adaptor between conjugating enzyme and 
            substrates.
  JOURNAL   J Biol Chem. 2001 Dec 28. 276(52):48973-7.
  PUBMED    11577116
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Dec 28. 276(52):48973-7."
COMMENT     Sequence Label: pT-36
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             20..42
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             50..82
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 cattcccctc tagaataatt ttgtttaact ttaagaagga gatatacata tggctagcat
       61 gactggtgga cagcaaatgg gtcgggatcc tatgagttta aaggaggaac aagtgtccat
      121 taagcaggac ccggaacaag aagagcgtca gcatgatcaa ttcaatgatg ttcagatcaa
      181 acaagagtcg caggatcatg atggagtgga ctcgcagtac acgaacggta cgcaaaatga
      241 cgacagcgag agatttgagg cagctgaatc cgatgtaaag gtggaacccg gcctcggtat
      301 gggcatcacc tcctctcaga gcgaaaaggg tcaagttctg cctgaccaac cggagattaa
      361 gttcattcgt cgtcaaatca atggttatgt agggtttgct aatttgccca agcaatggca
      421 cagaaggtcc ataaagaacg gattcagttt caatctccta tgtgtcggcc ctgatggtat
      481 tggtaagaca actttaatga aaactttatt taataatgat gatattgagg ccaatttagt
      541 gaaagattac gaggaggaac ttgcaaatga tcaggaagaa gaggagggtc agggcgaggg
      601 tcacgagaac caatcacaag aacaaaggca caaggtcaaa attaagtcat atgagtcggt
      661 tattgaagaa aatggtgtta aactgaattt gaacgtgatc gatactgaag gatttggtga
      721 ttttttacat aatgatcaaa aatcatggga ccccgatcat taaggagatt gactctcgtt
      781 ttg
//
