LOCUS       sequence-225330-         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector containing FASTKD4 mutant delta 226.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Wolf AR, Mootha VK
  TITLE     Functional genomic analysis of human mitochondrial RNA processing.
  JOURNAL   Cell Rep. 2014 May 8;7(3):918-31. doi: 10.1016/j.celrep.2014.03.035.
            Epub 2014 Apr 18.
  PUBMED    24746820
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.035"; journalName: "Cell Rep"; date: 
            "2014-05-8- 8"; volume: "7"; issue: "3"; pages: "918-31"
COMMENT     Sequence Label: pLex-FLAG-FASTKD4 mutant delta 226
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(67..90)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    122..146
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aaattttgta atccagaggt tgattgtcga cttaacgcgc caccggttag cgctagctca
       61 ttactacttg tcatcgtcat ccttgtaatc catagatcca ccagatccac cagatccacc
      121 aaccactttg tacaagaaag ttgggcactt ggccagctcc tcagccaccg ctttgcgcat
      181 cgcgtccgcg gcggcggcgc ctttctgcca ttcagacttg agttccagcc actcatagaa
      241 tgggacgtcc actatcagga agcctgcagc cactatgtgt cgccgggcca gaacaaagcg
      301 acccagcaag tccttgcttc ggctgttgaa gttggggaac tcccaccgca agaacgctag
      361 cctcttagac cctggaggtg gtgactggct cccagttggc tgggcaaggt gaggtgccac
      421 aaagtccctt acgggcagaa actcgccgtc actgtccagc agcacctcag catccagcac
      481 ccagccatac tgcgtggcca cctcgaggct gcccttgtcg gcgctcccca gcagcccctt
      541 cagcgtctcc tgcagctcct tttgcagggg ggtcaccttc ctgtcaaggg ctgagggccc
      601 aggggccaca gccgaggcag gcagaagggg acccgagtac tcggggtact ccagcagggc
      661 agtggcgttg atgtggagca gcttctggaa ggtgttctga tccttctgag acttgccccc
      721 tagaaattgg atgtgaaatt cggggtggag gacggcttgc agctctgctt cccgtgcctg
      781 ctgcagcaca cacagggccc acaccaggtc cacctgca
//
