LOCUS       sequence-225331-        4237 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene constitutive expression from a Pc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Xu Y, Tao F, Ma C, Xu P
  TITLE     New constitutive vectors: useful genetic engineering tools for 
            biocatalysis.
  JOURNAL   Appl Environ Microbiol. 2013 Apr;79(8):2836-40. doi: 
            10.1128/AEM.03746-12. Epub 2013 Feb 15.
  PUBMED    23416993
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03746-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-04"; volume: "79"; issue: "8"; pages: "2836-40"
COMMENT     Sequence Label: pBSPPc
FEATURES             Location/Qualifiers
     source          1..4237
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     228..244
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     229..245
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(279..295)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(279..295)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(332..350)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(371..387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(371..387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(384..406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    395..411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(419..425,426..443,444..449))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    464..485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(602..619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(773..1361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(853..872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1532..2323,2324..2392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2155..2174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2393..2497)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2565..2583
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2621..2643)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2743..2762
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      2824..3175
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     CDS             3189..4022
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     CDS             complement(join(4019..4237,1..375))
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSSKLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
                     PSTSRRVRYLHYSLQTEKAYVYWAKAFVLWTARSHGGFRHPREMGQAEVEGFLTMLATE
                     KQVAPATHRQALNALLFLYRQVPNSPYSESYYNSLAVVLQRRDWENPGVTQLNRLAAHP
                     PFASWRNSEEARTDRPSQQLRSLKGRPGRGGHGL"
     primer_bind     4168..4190
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4182..4199
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4183..4199
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4206..4225
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4206..4224
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctgccgatac aagaacaaca gcgcgttgag cgcctgccgg tgggtggccg gcgccacttg
       61 cttctcggtg gcgagcatgg tcagaaaacc ctcgacttca gcttgcccca tttcgcgcgg
      121 atgtcgaaac ccaccatggc tgcgggccgt ccacaacaca aatgccttgg cccagtagac
      181 ataagccttc tcggtctgta ggctgtaatg caggtagcga acccgtctcg aggtcgacgg
      241 tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc
      301 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
      361 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
      421 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
      481 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
      541 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
      601 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
      661 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
      721 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
      781 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
      841 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
      901 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
      961 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     1021 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     1081 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     1141 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     1201 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     1261 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     1321 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     1381 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     1441 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     1501 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     1561 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     1621 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     1681 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     1741 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     1801 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     1861 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     1921 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     1981 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     2041 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     2101 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     2161 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     2221 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     2281 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     2341 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     2401 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     2461 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     2521 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga
     2581 ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc
     2641 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     2701 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     2761 tactgagagt gcaccatacc tctcaggcgc cgctggtgcc gctggttgga cgccaagggt
     2821 gaatccgcct cgataccctg attactcgct tcctgcgccc tctcaggcgg cgatagggga
     2881 ctggtaaaac ggggattgcc cagacgcctc ccccgcccct tcaggggcac aaatgcggcc
     2941 ccaacggggc cacgtagtgg tgcgtttttt gcgtttccac ccttttcttc cttttccctt
     3001 ttaaaccttt taggacgtct acaggccacg taatccgtgg cctgtagagt ttaaaaaggg
     3061 acggatttgt tgccattaag ggacggattt gttgttaaga agggacggat ttgttgttgt
     3121 aaagggacgg atttgttgta ttgtgggacg cagatacagt gtccccttat acacaaggaa
     3181 tgtcgaacgt ggcctcaccc ccaatggttt acaaaagcaa tgccctggtc gaggccgcgt
     3241 atcgcctcag tgttcaggaa cagcggatcg ttctggcctg tattagccag gtgaagagga
     3301 gcgagcctgt caccgatgaa gtgatgtatt cagtgacggc ggaggacata gcgacgatgg
     3361 cgggtgtccc tatcgaatct tcctacaacc agctcaaaga agcggccctg cgcctgaaac
     3421 ggcgggaagt ccggttaacc caagagccca atggcaaggg gaaaagaccg agtgtgatga
     3481 ttaccggctg ggtgcaaaca atcatctacc gggagggtga gggccgtgta gaactcaggt
     3541 tcaccaaaga catgctgccg tacctgacgg aactcaccaa acagttcacc aaatacgcct
     3601 tggctgacgt ggccaagatg gacagcaccc acgcgatcag gctttacgag ctgctcatgc
     3661 aatgggacag catcggccag cgcgaaatag aaattgacca gctgcgaaag tggtttcaac
     3721 tggaaggccg gtatccctcg atcaaggact tcaagttgcg agtgcttgat ccagccgtga
     3781 cgcagatcaa cgagcacagc ccgctacagg tggagtgggc gcagcgaaag accgggcgca
     3841 aggtcacaca tctgttgttc agttttggac cgaagaagcc cgccaaggcg gtgggtaagg
     3901 ccccagcgaa gcgcaaggcc gggaagattt cagatgctga gatcgcgaaa caggctcgcc
     3961 ctggtgagac atgggaagcg gcccgcgctc gactaaccca gatgccgctg gatctggcct
     4021 agaggccgtg gccaccacgg cccggcctgc ctttcaggct gcgcaactgt tgggaagggc
     4081 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     4141 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     4201 aattgtaata cgactcacta tagggcgaat tgggtac
//
