LOCUS       sequence-225332-        5010 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene constitutive expression from a Pc promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Xu Y, Tao F, Ma C, Xu P
  TITLE     New constitutive vectors: useful genetic engineering tools for 
            biocatalysis.
  JOURNAL   Appl Environ Microbiol. 2013 Apr;79(8):2836-40. doi: 
            10.1128/AEM.03746-12. Epub 2013 Feb 15.
  PUBMED    23416993
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03746-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-04"; volume: "79"; issue: "8"; pages: "2836-40"
COMMENT     Sequence Label: pBSPPc-Gm
FEATURES             Location/Qualifiers
     source          1..5010
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(39..58)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(40..58)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(65..82)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(65..81)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(74..96)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             complement(242..1075)
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pRO1600 from Pseudomonas aeruginosa "
                     /label=pRO1600 Rep
                     /translation="MASPPMVYKSNALVEAAYRLSVQEQRIVLACISQVKRSEPVTDEV
                     MYSVTAEDIATMAGVPIESSYNQLKEAALRLKRREVRLTQEPNGKGKRPSVMITGWVQT
                     IIYREGEGRVELRFTKDMLPYLTELTKQFTKYALADVAKMDSTHAIRLYELLMQWDSIG
                     QREIEIDQLRKWFQLEGRYPSIKDFKLRVLDPAVTQINEHSPLQVEWAQRKTGRKVTHL
                     LFSFGPKKPAKAVGKAPAKRKAGKISDAEIAKQARPGETWEAARARLTQMPLDLA"
     rep_origin      1089..1440
                     /label=pRO1600 oriV
                     /note="broad-host-range origin of replication from 
                     Pseudomonas aeruginosa plasmid pRO1600; requires the 
                     pRO1600 Rep protein for replication (West et al., 1994)"
     primer_bind     complement(1502..1521)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1621..1643
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1681..1699)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1767..1871
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1872..1940,1941..2732)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2090..2109)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2903..3491
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3392..3411
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3645..3662
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3779..3800
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3815..3820,3821..3838,3839..3845)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3853..3869
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3858..3880
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3877..3893
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3877..3893
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        3914..3932
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        join(3983..3988,3989..4005,4006..4011)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             4200..4733
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     primer_bind     4742..4758
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4742..4758
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4792..4808)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4793..4809)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 acgcgctgtt gttcttgtat cggcaggtac ccaattcgcc ctatagtgag tcgtattaca
       61 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
      121 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
      181 atcgcccttc ccaacagttg cgcagcctga aaggcaggcc gggccgtggt ggccacggcc
      241 tctaggccag atccagcggc atctgggtta gtcgagcgcg ggccgcttcc catgtctcac
      301 cagggcgagc ctgtttcgcg atctcagcat ctgaaatctt cccggccttg cgcttcgctg
      361 gggccttacc caccgccttg gcgggcttct tcggtccaaa actgaacaac agatgtgtga
      421 ccttgcgccc ggtctttcgc tgcgcccact ccacctgtag cgggctgtgc tcgttgatct
      481 gcgtcacggc tggatcaagc actcgcaact tgaagtcctt gatcgaggga taccggcctt
      541 ccagttgaaa ccactttcgc agctggtcaa tttctatttc gcgctggccg atgctgtccc
      601 attgcatgag cagctcgtaa agcctgatcg cgtgggtgct gtccatcttg gccacgtcag
      661 ccaaggcgta tttggtgaac tgtttggtga gttccgtcag gtacggcagc atgtctttgg
      721 tgaacctgag ttctacacgg ccctcaccct cccggtagat gattgtttgc acccagccgg
      781 taatcatcac actcggtctt ttccccttgc cattgggctc ttgggttaac cggacttccc
      841 gccgtttcag gcgcagggcc gcttctttga gctggttgta ggaagattcg atagggacac
      901 ccgccatcgt cgctatgtcc tccgccgtca ctgaatacat cacttcatcg gtgacaggct
      961 cgctcctctt cacctggcta atacaggcca gaacgatccg ctgttcctga acactgaggc
     1021 gatacgcggc ctcgaccagg gcattgcttt tgtaaaccat tgggggtgag gccacgttcg
     1081 acattccttg tgtataaggg gacactgtat ctgcgtccca caatacaaca aatccgtccc
     1141 tttacaacaa caaatccgtc ccttcttaac aacaaatccg tcccttaatg gcaacaaatc
     1201 cgtccctttt taaactctac aggccacgga ttacgtggcc tgtagacgtc ctaaaaggtt
     1261 taaaagggaa aaggaagaaa agggtggaaa cgcaaaaaac gcaccactac gtggccccgt
     1321 tggggccgca tttgtgcccc tgaaggggcg ggggaggcgt ctgggcaatc cccgttttac
     1381 cagtccccta tcgccgcctg agagggcgca ggaagcgagt aatcagggta tcgaggcgga
     1441 ttcacccttg gcgtccaacc agcggcacca gcggcgcctg agaggtatgg tgcactctca
     1501 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     1561 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     1621 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     1681 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     1741 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     1801 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     1861 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     1921 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     1981 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     2041 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     2101 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     2161 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     2221 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     2281 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     2341 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     2401 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     2461 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     2521 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     2581 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     2641 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     2701 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     2761 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     2821 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     2881 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     2941 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     3001 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     3061 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     3121 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     3181 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     3241 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     3301 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     3361 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     3421 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     3481 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     3541 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     3601 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     3661 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     3721 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     3781 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     3841 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     3901 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggagctc ctgattccct
     3961 ttgtcaacag caatggatcg aattgacata agcctgttcg gttcgtaaac tgtaatgcaa
     4021 gtagcgtatg cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg
     4081 cgcagtggcg gttttcatgg cttgttatga ctgttttttt gtacagtcta tgcctcgggc
     4141 atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga
     4201 tgttacgcag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaggtg
     4261 gctcaagtat gggcatcatt cgcacatgta ggctcggccc tgaccaagtc aaatccatgc
     4321 gggctgctct tgatcttttc ggtcgtgagt tcggagacgt agccacctac tcccaacatc
     4381 agccggactc cgattacctc gggaacttgc tccgtagtaa gacattcatc gcgcttgctg
     4441 ccttcgacca agaagcggtt gttggcgctc tcgcggctta cgttctgccc aagtttgagc
     4501 agccgcgtag tgagatctat atctatgatc tcgcagtctc cggcgagcac cggaggcagg
     4561 gcattgccac cgcgctcatc aatctcctca agcatgaggc caacgcgctt ggtgcttatg
     4621 tgatctacgt gcaagcagat tacggtgacg atcccgcagt ggctctctat acaaagttgg
     4681 gcatacggga agaagtgatg cactttgata tcgacccaag taccgccacc taagcggccg
     4741 ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta tcgataccgt
     4801 cgacctcgag acgggttcgc tacctgcatt acagcctaca gaccgagaag gcttatgtct
     4861 actgggccaa ggcatttgtg ttgtggacgg cccgcagcca tggtgggttt cgacatccgc
     4921 gcgaaatggg gcaagctgaa gtcgagggtt ttctgaccat gctcgccacc gagaagcaag
     4981 tggcgccggc cacccaccgg caggcgctca
//
