LOCUS       sequence-225340-        5004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression vector containing FASTKD4 mutant delta 226.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 5004)
  AUTHORS   Wolf AR, Mootha VK
  TITLE     Functional genomic analysis of human mitochondrial RNA processing.
  JOURNAL   Cell Rep. 2014 May 8;7(3):918-31. doi: 10.1016/j.celrep.2014.03.035.
            Epub 2014 Apr 18.
  PUBMED    24746820
REFERENCE   2  (bases 1 to 5004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.03.035"; journalName: "Cell Rep"; date: 
            "2014-05-8- 8"; volume: "7"; issue: "3"; pages: "918-31"
COMMENT     Sequence Label: pLex-FLAG-FASTKD4 mutant delta 226
FEATURES             Location/Qualifiers
     source          1..5004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(1947..1971)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2003..2026
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    2068..2656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2121..2141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2539..2550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2728..2961
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3039..3160
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3076..3095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3130..3149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3200..3335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3355..3374)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3356..3374)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3384..3401)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3384..3400)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3393..3415)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3542..3997
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3629..3648)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3839..3860
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4023..4127
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4128..4196,4197..4988)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4346..4365)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 agagctctct ggctaactgt cgggatcaac aagtttgtac aaataagttg gcatggcagc
       61 tcacctggta aagcgatgca cgtgcctcct gagagaagct gctcgtcagg cccctgccat
      121 ggctccagtt ggccgactga gacttgcctg ggtagcccat aagactctga cttcctcagc
      181 cacctcaccc atttcccacc tcccaggttc cttgatggag ccggtggaga aggaacgagc
      241 atctactccc tacatagaga agcaggtgga ccacctcatc aagaaggcca caaggccaga
      301 ggagctcctg gagctacttg gtggcagtca cgacttggac agcaatcaag cagcaatggt
      361 acttatccgg ctctctcact tgctgtctga gaagccagaa gataaaggct tgctcataca
      421 ggatgcccac tttcatcaac ttctctgtct gctcaacagt cagattgcct cggtctggca
      481 tggtaccctc tcgaagctgc tgggaagcct gtatgctctg ggcatcccca aggcctccaa
      541 ggagctgcag tcggtggagc aggaggtccg ctggcgcatg cgcaaactga agtataagca
      601 cctggccttc ctggcagagt cctgtgccac cctctcacag gagcagcact cgcaggagct
      661 gctggctgag ctgctcacac acctggaaag gcgttggaca gaaattgaag attcccacac
      721 attagtgacc gtcatgatga aggtgggaca cctctcggag ccactaatga accgcctgga
      781 agacaagtgc ctggagttgg tggagcactt tggccccaat gagctgcgga aggtgctggt
      841 gatgctggca gctcagagcc ggcggtccgt gcccttgctg cgggccatct cctaccacct
      901 ggtgcagaag cccttctctc tgacgaaaga tgtgctcttg gacgtggcct atgcctatgg
      961 caaactcagc tttcaccaga cccaggtgtc ccagcgcctg gccaccgacc tgctatccct
     1021 catgcccagc ctgacttctg gtgaggtggc ccactgtgcc aagtccttcg ccttactcaa
     1081 gtggctcagc ctgcccctgt ttgaggcctt tgcccagcac gtcctgaaca gagcgcagga
     1141 catcaccctg ccccacctgt gcagcgtact tctggctttt gcgcgtctga acttccatcc
     1201 agaccaagag gatcagttct tcagcctggt acatgagaag ctggggtcag agctgccagg
     1261 cctggagcca gccctgcagg tggacctggt gtgggccctg tgtgtgctgc agcaggcacg
     1321 ggaagcagag ctgcaagccg tcctccaccc cgaatttcac atccaatttc tagggggcaa
     1381 gtctcagaag gatcagaaca ccttccagaa gctgctccac atcaacgcca ctgccctgct
     1441 ggagtacccc gagtactcgg gtccccttct gcctgcctcg gctgtggccc ctgggccctc
     1501 agcccttgac aggaaggtga cccccctgca aaaggagctg caggagacgc tgaaggggct
     1561 gctggggagc gccgacaagg gcagcctcga ggtggccacg cagtatggct gggtgctgga
     1621 tgctgaggtg ctgctggaca gtgacggcga gtttctgccc gtaagggact ttgtggcacc
     1681 tcaccttgcc cagccaactg ggagccagtc accacctcca gggtctaaga ggctagcgtt
     1741 cttgcggtgg gagttcccca acttcaacag ccgaagcaag gacttgctgg gtcgctttgt
     1801 tctggcccgg cgacacatag tggctgcagg cttcctgata gtggacgtcc cattctatga
     1861 gtggctggaa ctcaagtctg aatggcagaa aggcgccgcc gccgcggacg cgatgcgcaa
     1921 agcggtggct gaggagctgg ccaagtgccc aactttcttg tacaaagtgg ttggtggatc
     1981 tggtggatct ggtggatcta tggattacaa ggatgacgat gacaagtagt aatgagctag
     2041 cgctaaccgg tggcgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     2101 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     2161 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     2221 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     2281 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     2341 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     2401 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     2461 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     2521 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     2581 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     2641 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     2701 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     2761 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     2821 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     2881 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     2941 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     3001 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     3061 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     3121 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     3181 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     3241 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     3301 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     3361 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     3421 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     3481 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     3541 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     3601 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     3661 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     3721 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     3781 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     3841 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     3901 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     3961 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     4021 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     4081 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     4141 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     4201 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     4261 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     4321 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     4381 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     4441 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     4501 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     4561 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     4621 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     4681 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     4741 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     4801 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     4861 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     4921 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     4981 attggtaact gtcagaccaa gttt
//
