LOCUS       sequence-22535-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Dimitrov D, He Y, Mutoh H, Baker BJ, Cohen L, Akemann W, Knoepfel T
  TITLE     Engineering and characterization of an enhanced fluorescent protein 
            voltage sensor.
  JOURNAL   PLoS ONE. 2007  . 2(5):e440.
  PUBMED    17487283
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE. 
            2007  . 2(5):e440."
COMMENT     Sequence Label: pcDNA 3.1(-) VSFP2.1
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     51..70
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        51..69
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      89..98
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gcagagctct ctggctacta gagacccact gcttactggc ttatcgaaat taatacgact
       61 cactataggg agacccaagc tggctagcgc caccatggag ggattcgacg gttcagattt
      121 tagtcctcca gctgatttag ttggcgttga cggtgcagtc atgcggaacg tcgttgacgt
      181 cacgataaat ggtgacgtca ctgctccgcc gaaagctgcg ccaagaaaaa gtgaatcggt
      241 aaagaaagtt cattggaatg atgtagacca aggaccgagt gaaaaaccag agacaagaca
      301 ggaggaacga atagatatac ccgagatttc aggtctatgg tggggcgaga atgaacatgg
      361 agtggacgat gggagaatgg agatacctac tactggtgta ggtcgcgtcc agtttcgtgt
      421 ccgagcagtg attgatcatc tagggatgcg agtctttgga gtcttcctaa ttttcttgga
      481 catcatcctc atgatcattg atctcagtct tccaggaaaa agtgaatctt cacaatcctt
      541 ttatgacggg atggctttgg ctctttcttg ttatttcatg ctggatttag gattaaggat
      601 atttgcctac gggcccaaga atttcttcac caacccctgg gaggttgctg atggtttgat
      661 tatcgttgtc acattcgtcg tcacgatatt ttacactgtg ttagatgaat acgtgcaaga
      721 aacaggagcc gatggtttgg ggcagttggt tgtgttggcc cgtttgctgc gtgtggttcg
      781 attagcaag
//
