LOCUS       sequence-225401-        1182 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TetOFF (promoter-less) cassette with Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1182)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 1182)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1252_TetOFF(Hyg)
FEATURES             Location/Qualifiers
     source          1..1182
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             628..648
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      888..1140
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 gaattcatgg cccgcctgga taagtcaaag gtcatcaact ctgccttgga gctgctgaac
       61 gaggtcggca tcgagggtct cacaacgagg aagctggccc agaagctggg tgtcgagcag
      121 ccaaccctct actggcacgt caagaacaag cgcgccctcc tggacgccct cgccatcgag
      181 atgctggacc gccaccacac ccacttctgc cccctggagg gcgagagctg gcaggacttc
      241 ctgcgcaaca acgccaagag cttcaggtgc gccctcctga gccacaggga cggcgccaag
      301 gtccacctcg gcacccgccc caccgagaag cagtacgaga ccctggagaa ccagctcgcc
      361 ttcctgtgcc agcagggctt cagcctggag aacgccctct acgccctgag cgccgtcggt
      421 cacttcaccc tgggttgcgt cctggaggac caggagcacc aggtcgccaa ggaagagcgc
      481 gagaccccga ccaccgacag catgcccccg ctcctgcgcc aggccatcga gctgttcgac
      541 caccagggtg ccgagcccgc cttcctgttc ggccttgagc ttatcatctg cggcctggag
      601 aagcagctga agtgcgagag cggctcgccc aagaagaagc gcaaggtcga gggcagcgtc
      661 agcatgcagc caagcctcag gagcgagtac gagtacccgg tcttcagcca cgtccaggcc
      721 ggcatgttca gccccgagct gcgcaccttc accaagggtg acgccgagcg ctgggtcagc
      781 ggctcgccgg ccgacgccct ggatgatttc gacctggata tgctccctgc cgacgccctg
      841 gacgacttcg acctggatat gctgtgaggc gcggccggcc gcccggcgat cgttcaaaca
      901 tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg attatcatat
      961 aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg acgttattta
     1021 tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg atagaaaaca
     1081 aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg ttactagatc
     1141 cgatgataag ctgtcaaaca tgagaattgc ggccgcgtcg ac
//
