LOCUS       sequence-225403-         682 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TetOFF (promoter-less) cassette with Hygromycin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Yadav V, Yang F, Reza MH, Liu S, Valent B, Sanyal K, Naqvi NI
  TITLE     Cellular Dynamics and Genomic Identity of Centromeres in Cereal 
            Blast Fungus.
  JOURNAL   MBio. 2019 Jul 30;10(4). pii: mBio.01581-19. doi: 
            10.1128/mBio.01581-19.
  PUBMED    31363034
REFERENCE   2  (bases 1 to 682)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MBio."; 
            date: "2019-07-30"; volume: "10(4). pii: mBio.01581-19. doi"; pages:
            " 10.1128/mBio.01581-19"
COMMENT     Sequence Label: pFGL1252_TetOFF(Hyg)
FEATURES             Location/Qualifiers
     source          1..682
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    302..320
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
     protein_bind    508..526
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O1 for the tetR and tetA genes"
ORIGIN
        1 tctagataaa tcattccact caacattcag gctcctctgc gcacgtaaag tgccaaaggc
       61 aataccctgc tcggtggaat gccgccgggc ttgtcgattt tacgcacata ggcgcattct
      121 tgacttgaag cggaggagtt cttcgttgcg ggttacagtg ttttaataaa agaatggtca
      181 aatcaaactg ctagatatac ctgtcagaca ctctagttgt tgacccctat actcttaata
      241 catcagacag tacatgcatg ttgcatgatg atgtcggtac aactcccact agacatatcc
      301 tactctatca ttgatagagt gacccttcca tagacatatc ctactctatc attgatagat
      361 tgacccttcc actaggttca cttttctcta tcattgatag ggagtggtaa acaattgatg
      421 gacaaaatct atcaatgata gagagtgcat gttacctagt attgttcttc tctatcattg
      481 atagtgactt tgcctaatca cttagcaact ctatcattga tagagttcta gtattagcaa
      541 ggcctttcct cagatcccga tcaatccgac agccaaacct catccactct cactttcaca
      601 ctctaactta tacgatcact tctcgcccgt tcttttgaac atcaaatcaa ctaccttact
      661 tctatcaatt aatatgggta cc
//
