LOCUS       sequence-225430-       10387 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10387)
  AUTHORS   Deplancke B, Mukhopadhyay A, Ao W, Elewa AM, Grove CA, Martinez NJ, 
            Sequerra R, Doucette-Stamm L, Reece-Hoyes JS, Hope IA, Tissenbaum 
            HA, Mango SE, Walhout AJ
  TITLE     A gene-centered C. elegans protein-DNA interaction network.
  JOURNAL   Cell. 2006 Jun 16. 125(6):1193-205.
  PUBMED    16777607
REFERENCE   2  (bases 1 to 10387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2006 
            Jun 16. 125(6):1193-205."
COMMENT     Sequence Label: pMW#3
FEATURES             Location/Qualifiers
     source          1..10387
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    64..84
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(544..849)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYHLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(646..665)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(1169..1852)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     1767..1786
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(1853..1955)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     protein_bind    2112..2211
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(5574..5598)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(7138..7154)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7138..7154)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7151..7173)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7162..7178
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7186..7192,7193..7210,7211..7216))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7231..7252
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7369..7386)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7540..8128)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7620..7639)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8299..9090,9091..9159))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8922..8941
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9160..9264)
                     /gene="bla"
                     /label=AmpR promoter
     CDS             complement(9368..10171)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(10172..10387)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
ORIGIN
        1 aagcttggta ccgagctcgg atccactagt aacggccgcc agtgtgctgg aattcgccct
       61 tatcaacttt gtatagaaaa gttgaacgag aaacgtaaaa tgatataaat atcaatatat
      121 taaattagat tttgcataaa aaacagacta cataatactg taaaacacat ggtattagtg
      181 acctgtagaa ttcgagctcg accgacagcc ttccaaatgt tcttctcaaa cggaatcgtc
      241 gtatccagcc tactcgctat tgtcctcaat gccgtattaa atcataaaaa gaaataagaa
      301 aaagaggtgc gagcctcttt tttgtgtgac aaaataaaaa catctaccta ttcatatacg
      361 ctagtgtcat agtcctgaaa atcatctgca tcaagaacaa tttcacaact cttatacttt
      421 tctcttacaa gtcgttcggc ttcatctgga ttttcagcct ctatacttac taaacgtgat
      481 aaagtttctg taatttctac tgtatcgacc tgcagactgg ctgtgtataa gggagcctga
      541 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
      601 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
      661 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc
      721 cgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
      781 atcactctgt acatccacaa acagatgata acggctctct cttttatagg tgtaaacctt
      841 aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
      901 gggcgacctc agccgtccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac
      961 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt
     1021 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
     1081 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
     1141 cgcaaatacg catactgtta tctggctttt agtaagccgg atccacgcgg cgtttacgcc
     1201 ccccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc
     1261 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg
     1321 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta
     1381 aatcaaaact ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa
     1441 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt
     1501 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt
     1561 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt
     1621 tcattgccat acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg
     1681 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct
     1741 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac
     1801 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt
     1861 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat
     1921 tatggtgaaa gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg
     1981 cccagggctt cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt
     2041 ccgtcacagg tatttattcg gcgcaaagtg cgtcgggtga tgctgccaac ttagtcgacg
     2101 ctctagagct ccaaataatg attttatttt gactgatagt gacctgttcg ttgcaacaaa
     2161 ttgataagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc taagcttgaa
     2221 ttcgagctcg gtacccgggg atctgtcgac ctcgaggcat gtgctctgta tgtatataaa
     2281 actcttgttt tcttcttttc tctaaatatt ctttccttat acattaggtc ctttgtagca
     2341 taaattacta tacttctata gacacgcaaa cacaaataca cacactaaat taataatgac
     2401 cggatccgga gcttggctgt tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc
     2461 caatacgcaa accgcctctc cccgcgcgct ggccgattca ttaatgcagc tggcacgaca
     2521 ggtttcccga cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     2581 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt
     2641 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga
     2701 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta
     2761 caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac
     2821 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg
     2881 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg
     2941 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg
     3001 agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca
     3061 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac
     3121 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact
     3181 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt
     3241 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga
     3301 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact
     3361 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg
     3421 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg
     3481 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca
     3541 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa
     3601 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac
     3661 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat
     3721 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt
     3781 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa
     3841 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc
     3901 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg
     3961 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat
     4021 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca
     4081 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc
     4141 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga
     4201 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca
     4261 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc
     4321 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag
     4381 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg
     4441 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat
     4501 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt
     4561 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg
     4621 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct
     4681 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg
     4741 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct
     4801 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg
     4861 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc
     4921 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca
     4981 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg
     5041 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc
     5101 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt
     5161 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct
     5221 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg
     5281 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag
     5341 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga
     5401 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag
     5461 cccgtcagta tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg
     5521 gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaacccagc
     5581 tttcttgtac aaagttgata agggcgaatt ctgcagatgc tgacggaagc aaaacaccag
     5641 cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg
     5701 ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg
     5761 gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct
     5821 gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg
     5881 aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg
     5941 gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc
     6001 gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc
     6061 tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag
     6121 ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct
     6181 aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg
     6241 cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag
     6301 catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa
     6361 atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc
     6421 ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa
     6481 gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca
     6541 gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa
     6601 ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt
     6661 caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg
     6721 ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta
     6781 tcggcggaat tacagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa
     6841 taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac
     6901 tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta cttttttatc
     6961 atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca tatcagcaaa
     7021 agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt ccaaccgctg
     7081 tttggtctgc tttctgacaa actcggcctc gactctagcg aattaattcg taatcatgtc
     7141 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     7201 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     7261 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg
     7321 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
     7381 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
     7441 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
     7501 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
     7561 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
     7621 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
     7681 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
     7741 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
     7801 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
     7861 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
     7921 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
     7981 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
     8041 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
     8101 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     8161 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     8221 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     8281 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     8341 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     8401 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
     8461 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     8521 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     8581 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     8641 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     8701 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     8761 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     8821 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     8881 tatgcggcga ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag
     8941 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     9001 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     9061 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     9121 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     9181 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     9241 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg ggtaataact
     9301 gatataatta aattgaagct ctaatttgtg agtttagtat acatgcattt acttataata
     9361 cagtttttta gttttgctgg ccgcatcttc tcaaatatgc ttcccagcct gcttttctgt
     9421 aacgttcacc ctctacctta gcatcccttc cctttgcaaa tagtcctctt ccaacaataa
     9481 taatgtcaga tcctgtagag accacatcat ccacggttct atactgttga cccaatgcgt
     9541 ctcccttgtc atctaaaccc acaccgggtg tcataatcaa ccaatcgtaa ccttcatctc
     9601 ttccacccat gtctctttga gcaataaagc cgataacaaa atctttgtcg ctcttcgcaa
     9661 tgtcaacagt acccttagta tattctccag tagataggga gcccttgcat gacaattctg
     9721 ctaacatcaa aaggcctcta ggttcctttg ttacttcttc tgccgcctgc ttcaaaccgc
     9781 taacaatacc tgggcccacc acaccgtgtg cattcgtaat gtctgcccat tctgctattc
     9841 tgtatacacc cgcagagtac tgcaatttga ctgtattacc aatgtcagca aattttctgt
     9901 cttcgaagag taaaaaattg tacttggcgg ataatgcctt tagcggctta actgtgccct
     9961 ccatggaaaa atcagtcaag atatccacat gtgtttttag taaacaaatt ttgggaccta
    10021 atgcttcaac taactccagt aattccttgg tggtacgaac atccaatgaa gcacacaagt
    10081 ttgtttgctt ttcgtgcatg atattaaata gcttggcagc aacaggacta ggatgagtag
    10141 cagcacgttc cttatatgta gctttcgaca tgatttatct tcgtttcctg caggtttttg
    10201 ttctgtgcag ttgggttaag aatactgggc aatttcatgt ttcttcaaca ctacatatgc
    10261 gtatatatac caatctaagt ctgtgctcct tccttcgttc ttccttctgt tcggagatta
    10321 ccgaatcaaa aaaatttcaa ggaaaccgaa atcaaaaaaa agaataaaaa aaaaatgatg
    10381 aattgaa
//
