LOCUS       sequence-225460-        2334 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Circularly permutated firefly luciferase with SbMV cleavage site 
            (Luciferase reporter for detection of proteolytic activity).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala 
            N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R
  TITLE     Design of fast proteolysis-based signaling and logic circuits in 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 
            10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
  PUBMED    30531965
REFERENCE   2  (bases 1 to 2334)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0181-6"; journalName: "Nat Chem Biol"; date: 
            "2019-02"; volume: "15"; issue: "2"; pages: "115-122"
COMMENT     Sequence Label: cycLuc_SbMVs
FEATURES             Location/Qualifiers
     source          1..2334
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             2161..2208
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             2212..2331
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
ORIGIN
        1 gccaccatgc accaccacca ccaccacatg atcaaaatag ccacacgtaa atatttaggc
       61 aaacaaaatg tctatgacat tggagttgag cgcgaccata attttgcact caaaaatggc
      121 ttcatagctt ctaattgttt caatgatact tatagatata ttgacaccgc tatcctcagc
      181 gtggtgccat ttcaccacgg cttcggcatg ttcaccacgc tgggctactt gatctgcggc
      241 tttcgggtcg tgctcatgta ccgcttcgag gaggagctat tcttgcgcag cttgcaagac
      301 tataagattc aatctgccct gctggtgccc acactattta gcttcttcgc taagagcact
      361 ctcatcgaca agtacgacct aagcaacttg cacgagatcg ccagcggcgg ggcgccgctc
      421 agcaaggagg taggtgaggc cgtggccaaa cgcttccacc taccaggcat ccgccagggc
      481 tacggcctga cagaaacaac cagcgccatt ctgatcaccc ccgaagggga cgacaagcct
      541 ggcgcagtag gcaaggtggt gcccttcttc gaggctaagg tggtggactt ggacaccggt
      601 aagacactgg gtgtgaacca gcgcggcgag ctgtgcgtcc gtggccccat gatcatgagc
      661 ggctacgtta acaaccccga ggctacaaac gctctcatcg acaaggacgg ctggctgcac
      721 agcggcgaca tcgcctactg ggacgaggac gagcacttct tcatcgtgga ccggctgaag
      781 agcctgatca aatacaaggg ctaccaggta gccccagccg aactggagag catcctgctg
      841 caacacccca acatcttcga cgccggggtc gccggcctgc ccgacgacga tgccggcgag
      901 ctgcccgccg cagtcgtcgt gctggaacac ggtaaaacca tgaccgagaa ggagatcgtg
      961 gactatgtgg ccagccaggt tacaaccgcc aagaagctgc gcggtggtgt tgtgttcgtg
     1021 gacgaggtgc ctaaaggact gaccggcaag ttggacgccc gcaagatccg cgagattctc
     1081 attaaggcca agaagggatc cgagtccgtc agcctgcaaa gcggctcagg tgccaaaaac
     1141 attaagaagg gcccagcgcc attctaccca ctcgaagacg ggaccgccgg cgagcagctg
     1201 cacaaagcca tgaagcgcta cgccctggtg cccggcacca tcgcctttac cgacgcacat
     1261 atcgaggtgg acattaccta cgccgagtac ttcgagatga gcgttcggct ggcagaagct
     1321 atgaagcgct atgggctgaa tacaaaccat cggatcgtgg tgtgcagcga gaatagcttg
     1381 cagttcttca tgcccgtgtt gggtgccctg ttcatcggtg tggctgtggc cccagctaac
     1441 gacatctaca acgagcgcga gctgctgaac agcatgggca tcagccagcc caccgtcgta
     1501 ttcgtgagca agaaagggct gcaaaagatc ctcaacgtgc aaaagaagct accgatcata
     1561 caaaagatca tcatcatgga tagcaagacc gactaccagg gcttccaaag catgtacacc
     1621 ttcgtgactt cccatttgcc acccggcttc aacgagtacg acttcgtgcc cgagagcttc
     1681 gaccgggaca aaaccatcgc cctgatcatg aacagtagtg gcagtaccgg attgcccaag
     1741 ggcgtagccc taccgcaccg caccgcttgt gtccgattca gtcatgcccg cgaccccatc
     1801 ttcggcaacc agatcatccc cgccgagtac tgtttaagct atgaaacgga aatattgaca
     1861 gtagaatatg gattattacc gattggtaaa attgtagaaa agcgcatcga atgtactgtt
     1921 tatagcgttg ataataatgg aaatatttat acacaacctg tagcacaatg gcacgatcgc
     1981 ggagaacaag aggtgtttga gtattgtttg gaagatggtt cattgattcg ggcaacaaaa
     2041 gaccataagt ttatgactgt tgatggtcaa atgttgccaa ttgatgaaat atttgaacgt
     2101 gaattggatt tgatgcgggt tgataatttg ccgaacggcg gcaagatcgc cgtcaattct
     2161 gcttgcaaga actggttcag tagcttaagc cactttgtga tccaccttaa cagccacggc
     2221 ttccctcccg aggtggagga gcaggccgcc ggcaccctgc ccatgagctg cgcccaggag
     2281 agcggcatgg atagacaccc tgctgcttgc gccagcgcca ggatcaacgt ctaa
//
