LOCUS       sequence-225465-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus of sunflower mild mosaic virus protease 
            (SuMMVp) fused to the FKBP-rapamycin binding (FRB) domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala 
            N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R
  TITLE     Design of fast proteolysis-based signaling and logic circuits in 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 
            10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
  PUBMED    30531965
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0181-6"; journalName: "Nat Chem Biol"; date: 
            "2019-02"; volume: "15"; issue: "2"; pages: "115-122"
COMMENT     Sequence Label: FRB_nSuMMVp
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..63
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             64..342
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 gcgcgaattc gcggccgctc tagagccacc atggagcaga agctgatcag cgaggaggat
       61 ctgatcctct ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
      121 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
      181 cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
      241 gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcctccaa
      301 gcctgggacc tctattatca tgtgttccga cgaatctcaa agggatccgg aagtggcgtg
      361 agcctgagcc gcggcgtgcg cgactacaac gccatcagca gcatggtgtg ccgcgtgacc
      421 aacgacagcg gcagcagcag caccaccatg tacggcatcg gctacggctg ctacatcatc
      481 accaacaagc acctgttccg cgagaacaac ggccgcctgc tgatcaccag ccaccacggc
      541 gagtacatct gcaagaacag cgccagcctg aagctgagcc tggtgcccgg ccgcgacatg
      601 ctgctgatcc gcctgcccaa ggactgcccc cccttcccca gcaagctgaa gtttcgagag
      661 cccactagcg aagaaaaggc cgtgcttgta gtcacaaact tccaggagta aactagtgcg
      721 gccgcctgca gctcgaggcg c
//
