LOCUS       sequence-225466-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminus of soybean mosaic virus protease (SbMVp) fused 
            to the FK-506 binding protein (FKBP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala 
            N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R
  TITLE     Design of fast proteolysis-based signaling and logic circuits in 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 
            10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
  PUBMED    30531965
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0181-6"; journalName: "Nat Chem Biol"; date: 
            "2019-02"; volume: "15"; issue: "2"; pages: "115-122"
COMMENT     Sequence Label: FKBP_cSbMVp
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(31..33,34..354)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             742..768
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcgcgaattc gcggccgctc tagagccacc atgggagtgc aagtggaaac catctccccg
       61 ggagacgggc gcaccttccc caagcgcggc cagacctgcg tggtgcacta caccgggatg
      121 cttgaagatg gaaagaaatt tgattcctcc cgggacagaa acaagccctt taagtttatg
      181 ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt
      241 cagagagcca aactgactat atctccagat tatgcctatg gtgccactgg gcacccaggc
      301 atcatcccac cacatgccac tctcgtcttc gatgtggagc ttctaaaact ggaaggatcc
      361 ggaagtaaga gcctgcgcgc caccgtgagc gagagcagca tgatcctgcc cgagggcaag
      421 ggcagcttct ggatacactg gatcaccacc caggacggct tctgcggcct gcccctggtg
      481 agcgtgaacg acggccacat cgtgggcatc cacggcctga ccagcaacga cagcgagaag
      541 aacttcttcg tgcccctgac cgacggcttc gagaaggagt acctggagaa cgccgacaac
      601 ctgagctggg acaagcactg gttctgggag cccagcaaga tcgcctgggg cagcctgaac
      661 ctggtggagg agcagcccaa ggaggagttc aagatcagca agctggtgag cgacctgttc
      721 ggcaacaccg tgaccgtgca gtacccatac gatgttccag attacgctta aactagtgcg
      781 gccgcctgca gctcgaggcg c
//
