LOCUS       sequence-225467-         795 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses C-terminus of sunflower mild mosaic virus protease 
            (SuMMVp) fused to the FK-506 binding protein (FKBP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala 
            N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R
  TITLE     Design of fast proteolysis-based signaling and logic circuits in 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 
            10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
  PUBMED    30531965
REFERENCE   2  (bases 1 to 795)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0181-6"; journalName: "Nat Chem Biol"; date: 
            "2019-02"; volume: "15"; issue: "2"; pages: "115-122"
COMMENT     Sequence Label: FKBP_cSuMMVp
FEATURES             Location/Qualifiers
     source          1..795
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(31..33,34..354)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="MGVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNK
                     PFKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVEL
                     LKLE"
     CDS             736..762
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 gcgcgaattc gcggccgctc tagagccacc atgggagtgc aagtggaaac catctccccg
       61 ggagacgggc gcaccttccc caagcgcggc cagacctgcg tggtgcacta caccgggatg
      121 cttgaagatg gaaagaaatt tgattcctcc cgggacagaa acaagccctt taagtttatg
      181 ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt
      241 cagagagcca aactgactat atctccagat tatgcctatg gtgccactgg gcacccaggc
      301 atcatcccac cacatgccac tctcgtcttc gatgtggagc ttctaaaact ggaaggatcc
      361 ggaagtaagc atctttccag catggtgagc gagagcagct gcgtggtgca gcgcgaggac
      421 agccccatct ggcgccactg gatcagcacc aaggacggcc actgcggcgc ccccatcgtg
      481 agcatccgcg acggctacat catcggcagc cactgcggcg agaaccccat gaccagcaac
      541 ttcttcacca gcatccccaa ggacttccag aacctgctga acggcaagga ggccaacgag
      601 tgggtgagcg gctggaagta caacatcgac gccgtgtgct ggggcggcct gagcgtggtg
      661 aacgacgccc ccagcgagcc cttcatcacc gccaaggtgg tgagcgccct ggacaccgag
      721 ggcatcaagg tgcagtaccc atacgatgtt ccagattacg cttaaactag tgcggccgcc
      781 tgcagctcga ggcgc
//
