LOCUS       sequence-225468-        3306 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway vector for 3' tagging with rab5ab.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: p3E-rab5ab
FEATURES             Location/Qualifiers
     source          1..3306
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(1..125)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(792..887)
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(924..943)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(925..943)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(948..964)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(948..964)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1066..1085)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1077..1886
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1144..1163)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2033..2621
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2522..2541
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2775..2792
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2951..2978)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3070..3156)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3205..3227
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3219..3236
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3220..3236
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 catagtgact gcatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc
       61 taatttaata tattgatatt tatatcattt tacgtttctc gttcaacttt cttgtacaaa
      121 gtggtggcag gaagaggtgg agcaacgaga cctaacgggc cgaacaccgg caacaaaatc
      181 tgtcagttca aattagtgct gctgggagag tctgctgtgg ggaagtcgag tctagtgctg
      241 cgctttgtaa aaggacagtt tcatgagttt caggaaagca caataggagc cgccttcttg
      301 actcaaacat tatgtctgga tgacacaacg gcgaagtttg agatctggga tacagctggc
      361 caggagcgct accacagtct ggccccaatg tattacagag gagcacaggc ggccatagtg
      421 gtgtatgaca tcacaaatga agaatcattt gcacgtgcga aaaactgggt gaaagagctt
      481 caaagacaag ccagtccaaa tattgtaata gctttggctg ggaacaaggc agaccttgcc
      541 aacaagagag cgttggactt tcaggatgcg caatcatatg cagatgacaa cagtttgctc
      601 tttatggaaa cgtcggcaaa gacttcaatg aacgtcagtg agatttttat ggcgatcgca
      661 aagaagctcc cgaagaacga gccccagccc gcaggtgcca acacagcccg caacagagga
      721 gtggatctaa cagaaacagc acagcccgcc aaaggcccct gctgtagcac ctaaaccacg
      781 ctcctcacct ccaactttat tatacaaagt tggcattata aaaaagcatt gcttatcaat
      841 ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgga gctccatggt
      901 agcgttaacg cggccgcgat atcccctata gtgagtcgta ttacatggtc atagctgttt
      961 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     1021 tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga
     1081 gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat gctgatttat
     1141 atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt
     1201 atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc gttgccaatg
     1261 atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct cttccgacca
     1321 tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg atccccggaa
     1381 aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc
     1441 tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct tttaacagcg
     1501 atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg gttgatgcga
     1561 gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa gaaatgcata
     1621 aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca cttgataacc
     1681 ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc ggaatcgcag
     1741 accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct ccttcattac
     1801 agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa ttgcagtttc
     1861 atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa cactggcaga
     1921 gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt
     1981 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     2041 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     2101 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     2161 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact
     2221 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     2281 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     2341 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     2401 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     2461 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     2521 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2581 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     2641 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     2701 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     2761 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     2821 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     2881 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg
     2941 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt
     3001 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc
     3061 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa
     3121 ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc cctactctcg
     3181 cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg gccagtctta
     3241 agctcgggcc ctgcagctct agagctcgaa ttctacaggt cactaatacc atctaagtag
     3301 ttggtt
//
