LOCUS       sequence-225481-       11065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Multisite gateway destination vector for muscle specific expression 
            of constructs tagged at the 5' end with mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11065)
  AUTHORS   Hall TE, Martel N, Ariotti N, Xiong Z, Lo HP, Ferguson C, Rae J, Lim
            YW, Parton RG
  TITLE     In vivo cell biological screening identifies an endocytic capture 
            mechanism for T-tubule formation.
  JOURNAL   Nat Commun. 2020 Jul 24;11(1):3711. doi: 10.1038/s41467-020-17486-w.
  PUBMED    32709891
REFERENCE   2  (bases 1 to 11065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-07-24"; volume: "11(1):3711. doi"; pages: " 
            10.1038/s41467-020-17486-w"
COMMENT     Sequence Label: pDEST-actc1b-mKate2-ccdb
FEATURES             Location/Qualifiers
     source          1..11065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    3978..4002
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4003..4012
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4009..4704
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     protein_bind    complement(4706..4805)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        4962..5064
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5065..5748
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(5131..5150)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             6068..6373
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     6252..6271
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(6763..6886)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(6894..6910)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    6926..7060
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6950..6969)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7004..7023
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(7499..7686,7687..7698)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(7732..7748)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7732..7748)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7785..7805)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7785..7803)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7824..7840)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7837..7859)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7848..7864
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7872..7878,7879..7896,7897..7902))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7917..7938
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8055..8072)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8226..8814)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8306..8325)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8985..9776,9777..9845))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9608..9627
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9846..9950)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10113..10134)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(10483..10494,10495..10657)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     11030..11046
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caactttgta tagaaaagtt ggcacgcgca gattccgtgt gggcctggta atatcttacc
       61 attttaagct attttaagat tagggaaaaa aattaaataa aactacttta aaattacttt
      121 ccttaaaaag caacatattt atttagtttt tgtttaactg gctcaagatt gtaatatttt
      181 acttttacat gtttttttaa gtacctaaat gtgttcaaaa gtaaagtatg ttattgtttt
      241 ttttttttaa tgttagctaa gctttaaatt gtaatatatt acttttaaaa gtgacttttt
      301 aaaaatgtaa ataaaaaagt aactataaat gtgtaatgca tgttatttct gtgggttttt
      361 aattttcatc tcaactgggc ttacaatttt aacataataa aaatcaaatc aaatcaaatc
      421 aggcccttat ttgtcacata cactcttgta tactgaaata aactaaatgt tgagaaaaat
      481 ttaaatacac caaaataacg catacctttt ttgtaattac ctaagtaaaa catttagttc
      541 cttttttaag tgactcaaga ttgtaatcta ttaattgtaa aagataaaac tagtgatcaa
      601 tctgacgtgc ttctatgcag cctgtgagat taattgtgat gtttaattat taaacaattt
      661 aaatggatca aacatttcac atgtgcttat tgtgtccaac aaaaccagca gaagcactta
      721 aaaatattgc atgacaacat gagtttttaa tgtaaattat atatacttcc taaacgaagt
      781 taatcatatt tctaaaataa taatgtcatt gaaatattta aataaatgat aagctttagt
      841 tgattcaaca taaaattgaa agcaataagg actttttttt tttacagcgg tgaagcaaaa
      901 gcatgtaata atcatataat ataataaata aataaataaa taatggctct tggtgcaaat
      961 atgaacactt taaataaata tagaataaat atgatcaata agtcataacg atgtgtttta
     1021 tgttgattat aatttaaaca tgtttctaac gcaagtttta aaaactgttt taaagtatta
     1081 ttttaagtta taagatcaat ttgactcaac attttgtagt gaaaatatgt tgtgtaaaat
     1141 taatataata tacactataa aaatgcaggg ttccacacaa tacattaatg ttgtcccaac
     1201 acaaatcgat taagttaaca aatatatgtg gattgaacat aaaaaattaa gttgtcccaa
     1261 tgaaatctca agaattgtgt tgtttcagct cattttaaat aagtagtttt aacaagtaaa
     1321 aaataacata tttttgagtg tgtatatata tatattacaa aatataataa tataaaatgt
     1381 aaaagatata aaccatgcta catatattta agagctattt tgcacattac ttttttgcaa
     1441 gccattttag tgtttttttt acaaatatat attagctaaa tatacaatat cttaaatata
     1501 caattaatca gacatgcaat taatgcattt aacctacata aaaatacatg atcaacatat
     1561 gttttatgct gattacaact aaatcaaacg taatgaaatt aatttataaa agcgatttaa
     1621 atatgtcaat attttaagtt aaaatcactt gaaacctact ataaatgaac ttaaattaaa
     1681 aaaatctcct ttaattccaa tgcaacagtg ttgttttttc agctgaggac ccccggtgca
     1741 gatggttggc ccccggcccg ctattctaag gaagcctttc ttaattttag ggcggcgctg
     1801 catgcgctca gatcagattc acgggtggag agggttattt tctctttttt attttttctt
     1861 gcctgctcat atttgggcct gtgtgaaccc caggctccgc ctcggccccc aggagccgct
     1921 cttatttaaa ggggatcctc gccttcagtc ttcgtcccag tggaagcatc agtcttgtga
     1981 taacaacttg gtgagtccta cagaccagcg ctcgactcta ggctttaaat gcattgattt
     2041 ctctggatta gctaccggga gagttttaac tttgcttatt agtcttgtgc tcgaactgct
     2101 aggtttaaag agtgttttct ctaatgcatg catgaagcga cagctgaaaa ccagaaatag
     2161 agcgtttgtc ctcctgttaa actctagcct ttctttggca tttgtcagtc cgagtcttca
     2221 gctgccggca ggagattggg tttcagtaac acttgctgag cacaaactac acgtgctttc
     2281 ttcagattta attcattatt aatcattcta gatttaaatg atgttcttac tcattgctga
     2341 ttattttagc cttgcggagc agattaaatg agcacgtgtt tcattaagat tgatttaaat
     2401 ataattaata tttcaccact gtagttttaa aattgccttc taagtcatgg ttgattttta
     2461 ataggtatcg gctctaatta gattactatt ttggttttta aatgttattt ctatttcatt
     2521 tgtaggctcg tttttaatgt gattttatta gattctagtt ttaaaatcat ctcttagcct
     2581 taatgagcag actgaatgaa catgtttcat taagattaac ttcattataa tgaattattt
     2641 aatcattcta gtgggttata aatcagctct atttaaatta attgtcgtgt ttatttctat
     2701 ttaatttttg attatttttt cagggtcgtt tttatgtgat tttttcccgt ttctaatttt
     2761 aaactaattg tttgattttt atatgattcg actcttttta aatgaattgt tttgcttttt
     2821 ttattgagca tatatatatt atacaatttc aatcaacaat tgttgtgctt tttaaatgtt
     2881 gtttctattt catttctagg gtcgatttta ttcgattcta gttttaaaat cgtgattttt
     2941 aacttctaac cttactgagc agactgaatg aacatgtttc atcaagatta acttcattat
     3001 agttaattat tgaatcgtgc tagtggttta taaatcagct ctatttaaat taatcatcat
     3061 cattatttct atttaatttt tattatttct ttaggattat ttttatgtga tttttttcac
     3121 gttctagttt taaactcaat gctcattact gctcaccctt gctgagcagt ctttcgttaa
     3181 gattaaatta attaataacc attctagttt taaaacagtg tcttaaggca tagctgatat
     3241 tttacaagtt tactatttaa tatatctcct ttcttttttt agggtcgatt tcatctgatt
     3301 tgattaattt ttattatctt cactaagctt tttgttctag gttttattct cagtattatt
     3361 actgttatat ttgaactgaa tttctcctca tagtttattc attttattta tttatttttt
     3421 gtttattcgc tattttgtat tttagttttt aattagaaat tttattagaa tcgttttgaa
     3481 ctagttttta tgcactctaa cattaatatt aaatcataac tattattttt attataattt
     3541 atttgtgttt atctttttgt gtagctttca tgtgttttta ttttagtttt attatgtttg
     3601 cattgaattt tttggactag tttatataac gatatacaga aaagccatga caacaaatgt
     3661 ttttataata cctattaagt ataagctaat cagatttcca ctattctgtt caaagttaac
     3721 attattagcc attctgatgg atcatttgac atttgtcata tgtctttatg aacactgctc
     3781 tttcagatgc cacttttaaa gctgtttttt acagtaaatg aacgtttatt attatgacat
     3841 tatttcctaa ggcctctaga gtacagcagg atgtggcgtg tgcattaccg tgtaattatg
     3901 ctgactgtaa atgtgcattt atagactgtc agcatgtctg actgtgcgct gttgtgattg
     3961 gtcctgcaca gaccaaggca agtttgtaca aaaaagcagg ctgccaccat ggtgagcgag
     4021 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     4081 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     4141 aaggcggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     4201 tacggcagca aaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     4261 ttccccgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     4321 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     4381 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggcctcc
     4441 accgagaccc tgtaccccgc tgacggcggc ctggaaggca gagccgacat ggccctgaag
     4501 ctcgtgggcg ggggccacct gatctgcaac ttgaagacca catacagatc caagaaaccc
     4561 gctaagaacc tcaagatgcc cggcgtctac tatgtggaca gaagactgga aagaatcaag
     4621 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     4681 ctccctagca aactggggca cagagaccca gctttcttgt acaaagttgg cattataaaa
     4741 aagcattgct tatcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt
     4801 atttggagct ctagagcgtc gactaagttg gcagcatcac ccgacgcact ttgcgccgaa
     4861 taaatacctg tgacggaaga tcacttcgca gaataaataa atcctggtgt ccctgttgat
     4921 accgggaagc cctgggccaa cttttggcga aaatgagacg ttgatcggca cgtaagaggt
     4981 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     5041 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     5101 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     5161 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     5221 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     5281 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     5341 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     5401 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     5461 tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt
     5521 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     5581 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     5641 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     5701 gatgagtggc agggggggcg taaacgccgc gtggatccgg cttactaaaa gccagataac
     5761 agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat atgtataccc
     5821 gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag ttgacagcga
     5881 cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt aagcacaacc
     5941 atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa tcaggaaggg
     6001 atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga gaacaggggc
     6061 tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt
     6121 ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc ccctggccag
     6181 tgcacgtctg ctgtcggata aagtctcccg tgaactttac ccggtggtgc atatcgggga
     6241 tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga
     6301 agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt
     6361 ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc gatacagtag
     6421 aaattacaga aactttatca cgtttagtaa gtatagaggc tgaaaatcca gatgaagccg
     6481 aacgacttgt aagagaaaag tataagagtt gtgaaattgt tcttgatgca gatgattttc
     6541 aggactatga cactagcgta tatgaatagg tagatgtttt tattttgtca cacaaaaaag
     6601 aggctcgcac ctctttttct tatttctttt tatgatttaa tacggcattg aggacaatag
     6661 cgagtaggct ggatacgacg attccgtttg agaagaacat ttggaaggct gtcggtcgag
     6721 ctcgaattct acaggtcact aataccatct aagtagttga ttcatagtga ctgcatatgt
     6781 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     6841 tttatatcat tttacgtttc tcgttcaact ttattataca tagttgataa ttcactggcc
     6901 gtcgttttac ggtaccatcg atgatgatcc agacatgata agatacattg atgagtttgg
     6961 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     7021 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     7081 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     7141 caaatgtggt atggctgatt atgatcctct agatcagatc tgcgaagata cggccacggg
     7201 tgctcttgat cctgtggctg attttggact gtgctgctcg cagctgctga tgaatcacat
     7261 acttcctcca ttttcttcca ctgattgact gttataattt ccctaatttc caggtcaagg
     7321 tgctgtgcat tgtggtaata gatgtgacat gacgtcactt ccaaaggacc aatgaacatg
     7381 tctgaccaat ttcatataat gtgaaaacga ttttcatagg cagaataaat aacatttaaa
     7441 ttaaactggg catcagcgca attcaattgg tttggtaata gcaagggaaa atagaatgaa
     7501 gtgatctcca aaaaataagt actttttgac tgtaaataaa attgtaagga gtaaaaagta
     7561 cttttttttc taaaaaaatg taattaagta aaagtaaaag tattgatttt taattgtact
     7621 caagtaaagt aaaaatcccc aaaaataata cttaagtaca gtaatcaagt aaaattactc
     7681 aagtacttta cacctctggt tcttgacccc ctaccttcag caagcccagc agatccacta
     7741 gttctagagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     7801 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7861 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7921 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7981 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     8041 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     8101 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8161 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8221 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8281 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8341 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8401 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8461 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8521 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8581 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8641 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8701 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8761 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8821 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaaggcatt
     8881 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8941 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9001 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     9061 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9121 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9181 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9241 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9301 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9361 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9421 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9481 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9541 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9601 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9661 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9721 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9781 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9841 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9901 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9961 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
    10021 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
    10081 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
    10141 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccacgct
    10201 ggctaagaac tcatcagcct ccccggtcca tctacccacg taccaatgca ccaattggcc
    10261 acaatgacgg ctactacatg gtgccattcc ttcctcttta taggaatgga gactacctcc
    10321 tgtccaacaa tgctcttgga tacgagtacg cctacctgtt ggacccaggt cattgcacaa
    10381 caccagaaat gccctctgat ctgcaaaaga cgtgaatatc tgttcagaca cccatatcca
    10441 ctctgttcca cacaggtcag aggtttgtcc aggagttctt gacagaggtg taaaaagtac
    10501 tcaaaaattt tactcaagtg aaagtacaag tacttaggga aaattttact caattaaaag
    10561 taaaagtatc tggctagaat cttacttgag taaaagtaaa aaagtactcc attaaaattg
    10621 tacttgagta ttaaggaagt aaaagtaaaa gcaagaaaga aaactagaga ttcttgttta
    10681 agcttttaat ctcaaaaaac attaaatgaa atgcatacaa ggttttatcc tgctttagaa
    10741 ctgtttgtat ttaattatca aactataaga cagacaatct aatgccagta cacgctactc
    10801 aaagttgtaa aacctcagat ttaacttcag tagaagctga ttctcaaaat tgttagtgtc
    10861 aagcctagct cttttggggc tgaaaagcaa tcctgcagtg ctgaaaagcc tctcacaggc
    10921 agccgatgcg ggaagaggtg tattagtctt gatagagagg ctgcaaatag caggaaacgt
    10981 gagcagagac tccctggtgt ctgaaacaca ggccagatgg gccctcgagc aggaaacagc
    11041 tatgaccatg attacgccaa gctat
//
