LOCUS       sequence-225490-       14059 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes full-length human VEGFR-3 gene fused in-frame with 
            EGFP cloned in lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14059)
  TITLE     Alitalo expression plasmids
REFERENCE   2  (bases 1 to 14059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUW-hR3-EGFP
FEATURES             Location/Qualifiers
     source          1..14059
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1981..2000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2172..2551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2553..2751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2706..2726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2769..2949
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2996..3121
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3614..3847
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4032..4076
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4374..4491
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4556..5767
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5604..5623
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             join(9936..9938,9939..10652)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(9936..9938,9939..10649)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(9978..9999)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10239..10258)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     10586..10607
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10685..11273
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10738..10758)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11156..11167)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(11276..11292)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11277..11293)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11798..11978
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(12004..12021)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    12010..12234
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      12280..12708
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12367..12386)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12577..12598
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12717..12737)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12722..13051
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12902..13037
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12964..12983
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        13099..13146
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             13165..13539
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    13669..13790
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13706..13725)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13760..13779
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13839..13855)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13839..13855)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13852..13874)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13863..13879
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13887..13893,13894..13911,13912..13917))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13932..13953
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     2761 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2821 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2881 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2941 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     3001 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     3061 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     3121 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     3181 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     3241 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     3301 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     3361 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     3421 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     3481 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     3541 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     3601 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     3661 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     3721 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     3781 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     3841 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     3901 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     3961 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     4021 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     4081 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     4141 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     4201 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     4261 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     4321 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     4381 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     4441 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     4501 tacagggaca gcagagatcc agtttggtta attaacccgt gtcggctcca gatctggcct
     4561 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
     4621 tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
     4681 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     4741 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     4801 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     4861 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     4921 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg
     4981 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     5041 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
     5101 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
     5161 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta
     5221 atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt
     5281 gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg
     5341 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
     5401 gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta
     5461 ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca
     5521 ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt
     5581 atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc
     5641 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
     5701 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
     5761 gttagacgaa gcttgggctg caggtcgact ctagagccac catgcagcgg ggcgccgcgc
     5821 tgtgcctgcg actgtggctc tgcctgggac tcctggacgg cctggtgagt ggctactcca
     5881 tgaccccccc gaccttgaac atcacggagg agtcacacgt catcgacacc ggtgacagcc
     5941 tgtccatctc ctgcagggga cagcaccccc tcgagtgggc ttggccagga gctcaggagg
     6001 cgccagccac cggagacaag gacagcgagg acacgggggt ggtgcgagac tgcgagggca
     6061 cagacgccag gccctactgc aaggtgttgc tgctgcacga ggtacatgcc aacgacacag
     6121 gcagctacgt ctgctactac aagtacatca aggcacgcat cgagggcacc acggccgcca
     6181 gctcctacgt gttcgtgaga gactttgagc agccattcat caacaagcct gacacgctct
     6241 tggtcaacag gaaggacgcc atgtgggtgc cctgtctggt gtccatcccc ggcctcaatg
     6301 tcacgctgcg ctcgcaaagc tcggtgctgt ggccagacgg gcaggaggtg gtgtgggatg
     6361 accggcgggg catgctcgtg tccacgccac tgctgcacga tgccctgtac ctgcagtgcg
     6421 agaccacctg gggagaccag gacttccttt ccaacccctt cctggtgcac atcacaggca
     6481 acgagctcta tgacatccag ctgttgccca ggaagtcgct ggagctgctg gtaggggaga
     6541 agctggtcct gaactgcacc gtgtgggctg agtttaactc aggtgtcacc tttgactggg
     6601 actacccagg gaagcaggca gagcggggta agtgggtgcc cgagcgacgc tcccagcaga
     6661 cccacacaga actctccagc atcctgacca tccacaacgt cagccagcac gacctgggct
     6721 cgtatgtgtg caaggccaac aacggcatcc agcgatttcg ggagagcacc gaggtcattg
     6781 tgcatgaaaa tcccttcatc agcgtcgagt ggctcaaagg acccatcctg gaggccacgg
     6841 caggagacga gctggtgaag ctgcccgtga agctggcagc gtaccccccg cccgagttcc
     6901 agtggtacaa ggatggaaag gcactgtccg ggcgccacag tccacatgcc ctggtgctca
     6961 aggaggtgac agaggccagc acaggcacct acaccctcgc cctgtggaac tccgctgctg
     7021 gcctgaggcg caacatcagc ctggagctgg tggtgaatgt gcccccccag atacatgaga
     7081 aggaggcctc ctcccccagc atctactcgc gtcacagccg ccaggccctc acctgcacgg
     7141 cctacggggt gcccctgcct ctcagcatcc agtggcactg gcggccctgg acaccctgca
     7201 agatgtttgc ccagcgtagt ctccggcggc ggcagcagca agacctcatg ccacagtgcc
     7261 gtgactggag ggcggtgacc acgcaggatg ccgtgaaccc catcgagagc ctggacacct
     7321 ggaccgagtt tgtggaggga aagaataaga ctgtgagcaa gctggtgatc cagaatgcca
     7381 acgtgtctgc catgtacaag tgtgtggtct ccaacaaggt gggccaggat gagcggctca
     7441 tctacttcta tgtgaccacc atccccgacg gcttcaccat cgaatccaag ccatccgagg
     7501 agctactaga gggccagccg gtgctcctga gctgccaagc cgacagctac aagtacgagc
     7561 atctgcgctg gtaccgcctc aacctgtcca cgctgcacga tgcgcacggg aacccgcttc
     7621 tgctcgactg caagaacgtg catctgttcg ccacccctct ggccgccagc ctggaggagg
     7681 tggcacctgg ggcgcgccac gccacgctca gcctgagtat cccccgcgtc gcgcccgagc
     7741 acgagggcca ctatgtgtgc gaagtgcaag accggcgcag ccatgacaag cactgccaca
     7801 agaagtacct gtcggtgcag gccctggaag cccctcggct cacgcagaac ttgaccgacc
     7861 tcctggtgaa cgtgagcgac tcgctggaga tgcagtgctt ggtggccgga gcgcacgcgc
     7921 ccagcatcgt gtggtacaaa gacgagaggc tgctggagga aaagtctgga gtcgacttgg
     7981 cggactccaa ccagaagctg agcatccagc gcgtgcgcga ggaggatgcg ggacgctatc
     8041 tgtgcagcgt gtgcaacgcc aagggctgcg tcaactcctc cgccagcgtg gccgtggaag
     8101 gctccgagga taagggcagc atggagatcg tgatccttgt cggtaccggc gtcatcgctg
     8161 tcttcttctg ggtcctcctc ctcctcatct tctgtaacat gaggaggccg gcccacgcag
     8221 acatcaagac gggctacctg tccatcatca tggaccccgg ggaggtgcct ctggaggagc
     8281 aatgcgaata cctgtcctac gatgccagcc agtgggaatt cccccgagag cggctgcacc
     8341 tggggagagt gctcggctac ggcgccttcg ggaaggtggt ggaagcctcc gctttcggca
     8401 tccacaaggg cagcagctgt gacaccgtgg ccgtgaaaat gctgaaagag ggcgccacgg
     8461 ccagcgagca ccgcgcgctg atgtcggagc tcaagatcct cattcacatc ggcaaccacc
     8521 tcaacgtggt caacctcctc ggggcgtgca ccaagccgca gggccccctc atggtgatcg
     8581 tggagttctg caagtacggc aacctctcca acttcctgcg cgccaagcgg gacgccttca
     8641 gcccctgcgc ggagaagtct cccgagcagc gcggacgctt ccgcgccatg gtggagctcg
     8701 ccaggctgga tcggaggcgg ccggggagca gcgacagggt cctcttcgcg cggttctcga
     8761 agaccgaggg cggagcgagg cgggcttctc cagaccaaga agctgaggac ctgtggctga
     8821 gcccgctgac catggaagat cttgtctgct acagcttcca ggtggccaga gggatggagt
     8881 tcctggcttc ccgaaagtgc atccacagag acctggctgc tcggaacatt ctgctgtcgg
     8941 aaagcgacgt ggtgaagatc tgtgactttg gccttgcccg ggacatctac aaagaccctg
     9001 actacgtccg caagggcagt gcccggctgc ccctgaagtg gatggcccct gaaagcatct
     9061 tcgacaaggt gtacaccacg cagagtgacg tgtggtcctt tggggtgctt ctctgggaga
     9121 tcttctctct gggggcctcc ccgtaccctg gggtgcagat caatgaggag ttctgccagc
     9181 ggctgagaga cggcacaagg atgagggccc cggagctggc cactcccgcc atacgccgca
     9241 tcatgctgaa ctgctggtcc ggagacccca aggcgagacc tgcattctcg gagctggtgg
     9301 agatcctggg ggacctgctc cagggcaggg gcctgcaaga ggaagaggag gtctgcatgg
     9361 ccccgcgcag ctctcagagc tcagaagagg gcagcttctc gcaggtgtcc accatggccc
     9421 tacacatcgc ccaggctgac gctgaggaca gcccgccaag cctgcagcgc cacagcctgg
     9481 ccgccaggta ttacaactgg gtgtcctttc ccgggtgcct ggccagaggg gctgagaccc
     9541 gtggttcctc caggatgaag acatttgagg aattccccat gaccccaacg acctacaaag
     9601 gctctgtgga caaccagaca gacagtggga tggtgctggc ctcggaggag tttgagcaga
     9661 tagagagcag gcatagacaa gaaagcggct tcagctgtaa aggacctggc cagaatgtgg
     9721 ctgtgaccag ggcacaccct gactcccaag ggaggcggcg gcggcctgag cggggggccc
     9781 gaggaggcca ggtgttttac aacagcgagt atggggagct gtcggagcca agcgaggagg
     9841 accactgctc cccgtctgcc cgcgtgactt tcttcacaga caacagctac ggagggggag
     9901 gttctggcgg aggaggatcg ggtggtggtg gttcagtgag caagggcgag gagctgttca
     9961 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
    10021 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
    10081 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
    10141 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
    10201 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
    10261 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
    10321 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
    10381 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
    10441 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
    10501 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
    10561 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
    10621 tcactctcgg catggacgag ctgtacaagt agggcgcgcc gaattcgata tcaagcttat
    10681 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
    10741 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
    10801 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
    10861 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
    10921 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
    10981 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
    11041 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
    11101 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
    11161 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
    11221 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
    11281 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
    11341 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
    11401 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
    11461 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
    11521 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
    11581 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
    11641 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
    11701 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
    11761 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
    11821 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
    11881 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
    11941 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
    12001 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
    12061 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
    12121 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
    12181 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
    12241 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
    12301 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
    12361 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
    12421 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
    12481 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
    12541 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
    12601 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
    12661 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
    12721 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
    12781 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
    12841 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
    12901 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
    12961 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
    13021 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
    13081 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
    13141 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
    13201 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
    13261 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
    13321 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
    13381 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
    13441 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
    13501 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
    13561 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
    13621 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
    13681 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    13741 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
    13801 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
    13861 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    13921 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    13981 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    14041 ggtttgcgta ttgggcgct
//
