LOCUS       sequence-225493-       12615 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes full-length murine Tie1 gene cloned in lentiviral 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12615)
  TITLE     Alitalo expression plasmids
REFERENCE   2  (bases 1 to 12615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUW-mTie1
FEATURES             Location/Qualifiers
     source          1..12615
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1981..2000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2172..2551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2553..2751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2706..2726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2769..2949
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2996..3121
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3614..3847
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4032..4076
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4374..4491
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4556..5767
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5604..5623
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5797..5806
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    9241..9829
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9294..9314)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9712..9723)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9832..9848)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9833..9849)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10354..10534
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10560..10577)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10566..10790
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10836..11264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10923..10942)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11133..11154
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(11273..11293)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11278..11607
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11458..11593
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11520..11539
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11655..11702
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11721..12095
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12225..12346
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12262..12281)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12316..12335
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12395..12411)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12395..12411)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12408..12430)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12419..12435
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12443..12449,12450..12467,12468..12473))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12488..12509
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     2761 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2821 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2881 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2941 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     3001 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     3061 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     3121 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     3181 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     3241 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     3301 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     3361 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     3421 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     3481 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     3541 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     3601 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     3661 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     3721 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     3781 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     3841 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     3901 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     3961 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     4021 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     4081 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     4141 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     4201 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     4261 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     4321 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     4381 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     4441 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     4501 tacagggaca gcagagatcc agtttggtta attaacccgt gtcggctcca gatctggcct
     4561 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
     4621 tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
     4681 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     4741 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     4801 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     4861 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     4921 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg
     4981 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     5041 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
     5101 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
     5161 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta
     5221 atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt
     5281 gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg
     5341 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
     5401 gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta
     5461 ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca
     5521 ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt
     5581 atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc
     5641 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
     5701 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
     5761 gttagacgaa gcttgggctg caggtcgact ctagaggcca ccatggtctg gtggggatcc
     5821 tctttgctgc tccccactct tttcttggcc tctcatgttg gtgcgtctgt tgacttaaca
     5881 ttgctggcca acctgcgcat cacagacccc cagcgtttct tcctgacctg cgtgtctggt
     5941 gaggccggag cagggaggag ctcggacccg cccctgctgc tggagaagga tgatcgcatc
     6001 gtgcgcacct tcccgccggg gcagcccctg tacctggcac gcaacggttc tcaccaggtc
     6061 acgctgaggg gcttctccaa gccctcggat ttggtaggcg tcttctcctg tgtgggtggt
     6121 gctggcgcgc ggcgcactcg agttctctat gtgcacaaca gcccaggggc acacctgttt
     6181 ccagacaagg tcacacacac ggtgaacaaa ggtgacactg ctgtgctttc tgcccatgtg
     6241 cacaaggaaa agcagactga cgtgatctgg aagaacaacg gatcctactt caatacccta
     6301 gactggcaag aggccgatga cgggcgtttt caactgcagc tccaaaatgt acagccacca
     6361 tcaagtggca tctacagtgc cacctaccta gaggccagcc ccctgggcag tgccttcttt
     6421 cggctcatcg tgcgaggctg tggggctgga cgctgggggc caggatgtgt caaggattgc
     6481 ccgggctgcc tgcatggagg tgtctgtcat gaccatgatg gcgaatgtgt gtgtccccca
     6541 ggattcactg gcacccgctg tgagcaggcc tgcagggagg gtcgttttgg acagagctgt
     6601 caggaacagt gcccaggcac agcaggttgt agaggtctca ccttctgcct cccggatccc
     6661 tatggctgtt cctgtggatc tggctggaga ggaagccagt gccaggaagc atgcgcacct
     6721 ggtcattttg gggctgattg tcgcctccag tgccagtgtc aaaatggtgg cacttgtgac
     6781 cggttcagcg gctgcgtctg cccctctggg tggcatggag tccactgtga aaagtcagac
     6841 cggatccccc agatcctcag tatggccaca gaggtggagt tcaacatagg gacgatgccc
     6901 cggatcaact gtgcagctgc cgggaatccc ttcccagtgc ggggcagcat gaaacttcgc
     6961 aagccagatg gcaccatgct tttgtctacc aaagtcattg tggagccaga caggaccaca
     7021 gcagagtttg aagtgcccag tttgactctt ggggacagtg ggttctggga atgccgcgta
     7081 tcgacttctg gtggccaaga cagccggcgc ttcaaggtca atgtcaaagt acccccagtg
     7141 cctttgactg cacctcgact cctggccaag cagagccgtc agcttgtggt ctccccactg
     7201 gtctccttta gtggggatgg acccatctcc tctgtccgcc tgcactaccg gccccaggac
     7261 agcacgattg cctggtctgc cattgtggtg gatcccagtg agaatgtgac attaatgaac
     7321 ctgaagccaa agacaggata caatgtccga gtgcagctga gccggccagg ggaaggagga
     7381 gaaggaggct ggggaccttc tgcccttatg actactgact gtcctgagcc tttgctgcag
     7441 ccctggctag agagctggca cgtggagggt cctgacaggc tccgtgtgag ctggtctcta
     7501 ccctcggtgc cactgtccgg tgatggtttc ctgctgcgcc tgtgggatgg ggcccgggga
     7561 caggagaggc gggagaacat ctcattcccc caggcccgca ctgccctcct gactggactc
     7621 acgccgggca cccactacca gctggatgtg cgactgtatc actgtaccct cctgggccct
     7681 gcctcacccc ctgcgcatgt gcacctgccc cccagcgggc ctccagctcc ccgccacctc
     7741 catgcccagg ccctctcaga ctctgagatt caactcatgt ggcagcatcc cgaggctccg
     7801 tctgggccta tatccaagta catcgtggag attcaggtgg ccgggggctc aggagaccca
     7861 cagtggatgg atgtagacag gcccgaggag acaagcatca ttgttcgtgg cctcaatgct
     7921 agcacacgat atcttttccg tgtgcgggcc agtgtgcagg gtcttggcga ctggagcaat
     7981 acagtagaag aggccactct gggcaatggg ctgcagagtg aggacccagt ccgagaaagc
     8041 cgggcagctg aagaaggcct ggatcagcag ctggtcctgg ctgtggtagg ttccgtctct
     8101 gccacctgcc tcaccatcct ggctgccctt ttagccttgg tgtgtatccg aagaagctgc
     8161 ctacatcgga gacgcacctt cacttaccag tcaggatcgg gtgaagagac catcctgcag
     8221 ttcagctcag ggaccttgac cttgacccgg cgaccgaagc cacagcctga gcccttgagt
     8281 taccccgtgc tggagtggga ggacatcacc tttgaggacc tcatagggga gggcaacttt
     8341 ggccaagtga tccgggccat gatcaagaag gacgggctca agatgaacgc agccatcaag
     8401 atgctaaaag agtatgcgtc tgaaaatgac catcgagact ttgcaggtga actagaagtt
     8461 ctgtgcaaac taggacacca ccccaatatt atcaacctct tgggggcctg tgagaaccga
     8521 ggctacttgt atattgctat cgaatacgcc ccctatggaa acctactgga cttcctgaga
     8581 aagagcaggg tcctggagac tgatccagct tttgctcgag agcatgggac ggcctccaca
     8641 ctcagctctc ggcagctgct gcgctttgcc agtgatgcag ccaacggcat gcagtacctt
     8701 agtgagaagc agttcattca cagggacctg gctgcccgaa atgtgctggt cggagagaac
     8761 ctggcctcca agattgcaga ctttggcctt tctcgagggg aggaggtgta tgtgaagaag
     8821 acaatgggcc gtctccctgt gcgttggatg gccattgagt ccctcaacta cagcgtttat
     8881 accaccaaga gtgatgtctg gtcctttggg gtcctcctct gggagatagt gagccttgga
     8941 ggcacgccct actgtggcat gacctgtgct gagctctacg agaagctgcc tcaaggctat
     9001 cgcatggagc agcctcgaaa ctgcgatgac gaagtgtacg agctgatgcg gcagtgctgg
     9061 cgggatcggc cctatgagcg cccccccttt gctcagatcg cactacagtt gggccgcatg
     9121 ctggaagcca ggaaggccta cgtgaacatg tcgctgtttg agaacttcac ctatgcgggc
     9181 atcgatgcca cagctgagga ggcctagggg cgcgccgaat tcgatatcaa gcttatcgat
     9241 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     9301 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     9361 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     9421 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     9481 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     9541 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     9601 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     9661 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     9721 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     9781 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     9841 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     9901 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     9961 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    10021 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
    10081 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
    10141 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
    10201 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
    10261 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
    10321 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
    10381 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
    10441 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
    10501 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
    10561 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
    10621 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
    10681 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
    10741 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
    10801 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
    10861 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
    10921 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
    10981 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
    11041 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
    11101 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
    11161 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
    11221 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
    11281 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
    11341 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    11401 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    11461 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    11521 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
    11581 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
    11641 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
    11701 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
    11761 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
    11821 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
    11881 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
    11941 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
    12001 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
    12061 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
    12121 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    12181 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    12241 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    12301 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    12361 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    12421 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    12481 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    12541 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    12601 tgcgtattgg gcgct
//
