LOCUS       sequence-225495-       13375 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid encodes full-length human Tie1 gene fused in-frame with 
            Cherry cloned in lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13375)
  TITLE     Alitalo expression plasmids
REFERENCE   2  (bases 1 to 13375)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FUW-TIE1-Cherry
FEATURES             Location/Qualifiers
     source          1..13375
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(11..28)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(182..770)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(262..281)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(941..1732,1733..1801))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1564..1583
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1802..1906)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1981..2000)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2172..2551
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2553..2751
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2706..2726
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2769..2949
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2996..3121
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3614..3847
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4032..4076
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4374..4491
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4556..5767
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     5604..5623
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     regulatory      5796..5805
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(6339..6350)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             9261..9968
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(9403..9421)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     9671..9690
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    10001..10589
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10054..10074)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10472..10483)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10592..10608)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10593..10609)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             11114..11294
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(11320..11337)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11326..11550
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11596..12024
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11683..11702)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11893..11914
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12033..12053)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        12038..12367
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      12218..12353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     12280..12299
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        12415..12462
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             12481..12855
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    12985..13106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13022..13041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13076..13095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13155..13171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13155..13171)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(13168..13190)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    13179..13195
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(13203..13209,13210..13227,13228..13233))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13248..13269
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
       61 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
      121 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
      181 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
      241 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
      301 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
      361 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
      421 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
      481 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
      541 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
      601 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
      661 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
      721 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
      781 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
      841 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
      901 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
      961 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     1021 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     1081 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     1141 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     1201 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     1261 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     1321 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     1381 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     1441 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     1501 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     1561 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     1621 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     1681 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     1741 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     1801 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     1861 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     1921 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     1981 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     2041 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     2101 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     2161 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     2221 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     2281 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     2341 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     2401 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     2461 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     2521 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     2581 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     2641 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     2701 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     2761 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     2821 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     2881 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     2941 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     3001 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     3061 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     3121 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     3181 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     3241 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     3301 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     3361 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     3421 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     3481 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     3541 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     3601 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     3661 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     3721 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     3781 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     3841 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     3901 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     3961 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     4021 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     4081 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     4141 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     4201 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     4261 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     4321 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     4381 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     4441 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     4501 tacagggaca gcagagatcc agtttggtta attaacccgt gtcggctcca gatctggcct
     4561 ccgcgccggg ttttggcgcc tcccgcgggc gcccccctcc tcacggcgag cgctgccacg
     4621 tcagacgaag ggcgcagcga gcgtcctgat ccttccgccc ggacgctcag gacagcggcc
     4681 cgctgctcat aagactcggc cttagaaccc cagtatcagc agaaggacat tttaggacgg
     4741 gacttgggtg actctagggc actggttttc tttccagaga gcggaacagg cgaggaaaag
     4801 tagtcccttc tcggcgattc tgcggaggga tctccgtggg gcggtgaacg ccgatgatta
     4861 tataaggacg cgccgggtgt ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg
     4921 ttcttgtttg tggatcgctg tgatcgtcac ttggtgagta gcgggctgct gggctggccg
     4981 gggctttcgt ggccgccggg ccgctcggtg ggacggaagc gtgtggagag accgccaagg
     5041 gctgtagtct gggtccgcga gcaaggttgc cctgaactgg gggttggggg gagcgcagca
     5101 aaatggcggc tgttcccgag tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg
     5161 ttgaaacaag gtggggggca tggtgggcgg caagaaccca aggtcttgag gccttcgcta
     5221 atgcgggaaa gctcttattc gggtgagatg ggctggggca ccatctgggg accctgacgt
     5281 gaagtttgtc actgactgga gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgg
     5341 cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg cgccctcgtc gtgtcgtgac
     5401 gtcacccgtt ctgttggctt ataatgcagg gtggggccac ctgccggtag gtgtgcggta
     5461 ggcttttctc cgtcgcagga cgcagggttc gggcctaggg taggctctcc tgaatcgaca
     5521 ggcgccggac ctctggtgag gggagggata agtgaggcgt cagtttcttt ggtcggtttt
     5581 atgtacctat cttcttaagt agctgaagct ccggttttga actatgcgct cggggttggc
     5641 gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg taatcatttg ggtcaatatg
     5701 taattttcag tgttagacta gtaaattgtc cgctaaattc tggccgtttt tggctttttt
     5761 gttagacgaa gcttgggctg caggtcgact ctagagccac catggtctgg cgggtgcccc
     5821 ctttcttgct ccccatcctc ttcttggctt ctcatgtggg cgcggcggtg gacctgacgc
     5881 tgctggccaa cctgcggctc acggaccccc agcgcttctt cctgacttgc gtgtctgggg
     5941 aggccggggc ggggaggggc tcggacgcct ggggcccgcc cctgctgctg gagaaggacg
     6001 accgtatcgt gcgcaccccg cccgggccac ccctgcgcct ggcgcgcaac ggttcgcacc
     6061 aggtcacgct tcgcggcttc tccaagccct cggacctcgt gggcgtcttc tcctgcgtgg
     6121 gcggtgctgg ggcgcggcgc acgcgcgtca tctacgtgca caacagccct ggagcccacc
     6181 tgcttccaga caaggtcaca cacactgtga acaaaggtga caccgctgta ctttctgcac
     6241 gtgtgcacaa ggagaagcag acagacgtga tctggaagag caacggatcc tacttctaca
     6301 ccctggactg gcatgaagcc caggatgggc ggttcctgct gcagctccca aatgtgcagc
     6361 caccatcgag cggcatctac agtgccactt acctggaagc cagccccctg ggcagcgcct
     6421 tctttcggct catcgtgcgg ggttgtgggg ctgggcgctg ggggccaggc tgtaccaagg
     6481 agtgcccagg ttgcctacat ggaggtgtct gccacgacca tgacggcgaa tgtgtatgcc
     6541 cccctggctt cactggcacc cgctgtgaac aggcctgcag agagggccgt tttgggcaga
     6601 gctgccagga gcagtgccca ggcatatcag gctgccgggg cctcaccttc tgcctcccag
     6661 acccctatgg ctgctcttgt ggatctggct ggagaggaag ccagtgccaa gaagcttgtg
     6721 cccctggtca ttttggggct gattgccgac tccagtgcca gtgtcagaat ggtggcactt
     6781 gtgaccggtt cagtggttgt gtctgcccct ctgggtggca tggagtgcac tgtgagaagt
     6841 cagaccggat cccccagatc ctcaacatgg cctcagaact ggagttcaac ttagagacga
     6901 tgccccggat caactgtgca gctgcaggga accccttccc cgtgcggggc agcatagagc
     6961 tacgcaagcc agacggcact gtgctcctgt ccaccaaggc cattgtggag ccagagaaga
     7021 ccacagctga gttcgaggtg ccccgcttgg ttcttgcgga cagtgggttc tgggagtgcc
     7081 gtgtgtccac atctggcggc caagacagcc ggcgcttcaa ggtcaatgtg aaagtgcccc
     7141 ccgtgcccct ggctgcacct cggctcctga ccaagcagag ccgccagctt gtggtctccc
     7201 cgctggtctc gttctctggg gatggaccca tctccactgt ccgcctgcac taccggcccc
     7261 aggacagtac catggactgg tcgaccattg tggtggaccc cagtgagaac gtgacgttaa
     7321 tgaacctgag gccaaagaca ggatacagtg ttcgtgtgca gctgagccgg ccaggggaag
     7381 gaggagaggg ggcctggggg cctcccaccc tcatgaccac agactgtcct gagcctttgt
     7441 tgcagccgtg gttggagggc tggcatgtgg aaggcactga ccggctgcga gtgagctggt
     7501 ccttgccctt ggtgcccggg ccactggtgg gcgacggttt cctgctgcgc ctgtgggacg
     7561 ggacacgggg gcaggagcgg cgggagaacg tctcatcccc ccaggcccgc actgccctcc
     7621 tgacgggact cacgcctggc acccactacc agctggatgt gcagctctac cactgcaccc
     7681 tcctgggccc ggcctcgccc cctgcacacg tgcttctgcc ccccagtggg cctccagccc
     7741 cccgacacct ccacgcccag gccctctcag actccgagat ccagctgaca tggaagcacc
     7801 cggaggctct gcctgggcca atatccaagt acgttgtgga ggtgcaggtg gctgggggtg
     7861 caggagaccc actgtggata gacgtggaca ggcctgagga gacaagcacc atcatccgtg
     7921 gcctcaacgc cagcacgcgc tacctcttcc gcatgcgggc cagcattcag gggctcgggg
     7981 actggagcaa cacagtagaa gagtccaccc tgggcaacgg gctgcaggct gagggcccag
     8041 tccaagagag ccgggcagct gaagagggcc tggatcagca gctgatcctg gcggtggtgg
     8101 gctccgtgtc tgccacctgc ctcaccatcc tggccgccct tttaaccctg gtgtgcatcc
     8161 gcagaagctg cctgcatcgg agacgcacct tcacctacca gtcaggctcg ggcgaggaga
     8221 ccatcctgca gttcagctca gggaccttga cacttacccg gcggccaaaa ctgcagcccg
     8281 agcccctgag ctacccagtg ctagagtggg aggacatcac ctttgaggac ctcatcgggg
     8341 aggggaactt cggccaggtc atccgggcca tgatcaagaa ggacgggctg aagatgaacg
     8401 cagccatcaa aatgctgaaa gagtatgcct ctgaaaatga ccatcgtgac tttgcgggag
     8461 aactggaagt tctgtgcaaa ttggggcatc accccaacat catcaacctc ctgggggcct
     8521 gtaagaaccg aggttacttg tatatcgcta ttgaatatgc cccctacggg aacctgctag
     8581 attttctgcg gaaaagccgg gtcctagaga ctgacccagc ttttgctcga gagcatggga
     8641 cagcctctac ccttagctcc cggcagctgc tgcgtttcgc cagtgatgcg gccaatggca
     8701 tgcagtacct gagtgagaag cagttcatcc acagggacct ggctgcccgg aacgtgctgg
     8761 tcggagagaa cctagcctcc aagattgcag acttcggcct ttctcgggga gaggaggttt
     8821 atgtgaagaa gacgatgggg cgtctccctg tgcgctggat ggccattgag tccctgaact
     8881 acagtgtcta taccaccaag agtgatgtct ggtcctttgg agtccttctt tgggagatag
     8941 tgagccttgg aggtacaccc tactgtggca tgacctgtgc cgagctctat gaaaagctgc
     9001 cccagggcta ccgcatggag cagcctcgaa actgtgacga tgaagtgtac gagctgatgc
     9061 gtcagtgctg gcgggaccgt ccctatgagc gacccccctt tgcccagatt gcgctacagc
     9121 taggccgcat gctggaagcc aggaaggcct atgtgaacat gtcgctgttt gagaacttca
     9181 cttacgcggg cattgatgcc acagctgagg aggccggagg gggaggttct ggcggaggag
     9241 gatcgggtgg tggtggttca gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     9301 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     9361 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     9421 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     9481 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     9541 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     9601 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     9661 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     9721 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     9781 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     9841 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     9901 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     9961 acaagtaagg cgcgccgaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
    10021 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
    10081 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
    10141 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
    10201 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
    10261 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
    10321 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
    10381 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
    10441 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
    10501 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
    10561 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
    10621 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
    10681 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
    10741 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
    10801 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
    10861 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
    10921 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
    10981 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
    11041 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
    11101 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
    11161 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
    11221 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
    11281 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
    11341 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
    11401 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
    11461 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
    11521 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
    11581 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
    11641 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
    11701 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
    11761 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
    11821 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
    11881 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
    11941 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
    12001 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
    12061 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
    12121 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
    12181 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
    12241 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
    12301 cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
    12361 ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac
    12421 aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc
    12481 atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc
    12541 gagttctgga ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt
    12601 gtggtccggg acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac
    12661 aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag
    12721 gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag
    12781 ccgtgggggc gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc
    12841 gaggagcagg actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg
    12901 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
    12961 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
    13021 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
    13081 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
    13141 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    13201 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    13261 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
    13321 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgct
//
