LOCUS       sequence-225504-       10318 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, 
            contains artificial signal sequence upstream of tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10318)
  AUTHORS   Lai MB, Zhang C, Shi J, Johnson V, Khandan L, McVey J, Klymkowsky 
            MW, Chen Z, Junge HJ
  TITLE     TSPAN12 Is a Norrin Co-receptor that Amplifies Frizzled4 Ligand 
            Selectivity and Signaling.
  JOURNAL   Cell Rep. 2017 Jun 27;19(13):2809-2822. doi: 
            10.1016/j.celrep.2017.06.004.
  PUBMED    28658627
REFERENCE   2  (bases 1 to 10318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.06"; journalName: "Cell Rep"; date: 
            "2017-06-27- 27"; volume: "19"; issue: "13"; pages: "2809-2822"
COMMENT     Sequence Label: pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)
FEATURES             Location/Qualifiers
     source          1..10318
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        50..429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        430..633
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     584..604
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     630..654
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             825..890
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     primer_bind     complement(5704..5723)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    5748..5796
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      5998..6426
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6085..6104)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6295..6316
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6435..6455)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6440..6769
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6620..6755
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6682..6701
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6836..7630
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6890..6909)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7500..7519
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7806..7927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7843..7862)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7897..7916
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7976..7992)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7976..7992)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7989..8011)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8000..8016
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8024..8030,8031..8048,8049..8054))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8069..8090
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8207..8224)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8378..8966)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8458..8477)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9137..9928,9929..9997))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9760..9779
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9998..10102)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10177..10196)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gttaggcgtt ttgcgctgct tcgcgatgta cgggccagat atacgcgttg acattgatta
       61 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
      121 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      181 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
      241 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
      301 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
      361 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
      421 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
      481 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
      541 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
      601 cgtgtacggt gggaggtcta tataagcaga gctcgtttag tgaaccgtca gatcgcctgg
      661 agacgccatc cacgctgttt tgacctccat agaagacacc gggaccgatc cagcctccgg
      721 actctagagg atcgaaccct tccaccatgg gagggactgc cgccaggttg ggggccgtga
      781 ttttgtttgt cgtcatagtg ggcctccatg gggtccgcgg caaagactac aaagaccatg
      841 acggtgatta taaagatcat gacatagatt acaaggatga cgatgacaag gctagctcgc
      901 cgctcctgct atttgccaac cgccgggacg tacggctggt ggacgccggc ggagtcaagc
      961 tggagtccac catcgtggtc agcggcctgg aggatgcggc cgcagtggac ttccagtttt
     1021 ccaagggagc cgtgtactgg acagacgtga gcgaggaggc catcaagcag acctacctga
     1081 accagacggg ggccgccgtg cagaacgtgg tcatctccgg cctggtctct cccgacggcc
     1141 tcgcctgcga ctgggtgggc aagaagctgt actggacgga ctcagagacc aaccgcatcg
     1201 aggtggccaa cctcaatggc acatcccgga aggtgctctt ctggcaggac cttgaccagc
     1261 cgagggccat cgccttggac cccgctcacg ggtacatgta ctggacagac tggggtgaga
     1321 cgccccggat tgagcgggca gggatggatg gcagcacccg gaagatcatt gtggactcgg
     1381 acatttactg gcccaatgga ctgaccatcg acctggagga gcagaagctc tactgggctg
     1441 acgccaagct cagcttcatc caccgtgcca acctggacgg ctcgttccgg cagaaggtgg
     1501 tggagggcag cctgacgcac cccttcgccc tgacgctctc cggggacact ctgtactgga
     1561 cagactggca gacccgctcc atccatgcct gcaacaagcg cactgggggg aagaggaagg
     1621 agatcctgag tgccctctac tcacccatgg acatccaggt gctgagccag gagcggcagc
     1681 ctttcttcca cactcgctgt gaggaggaca atggcggctg ctcccacctg tgcctgctgt
     1741 ccccaagcga gcctttctac acatgcgcct gccccacggg tgtgcagctg caggacaacg
     1801 gcaggacgtg taaggcagga gccgaggagg tgctgctgct ggcccggcgg acggacctac
     1861 ggaggatctc gctggacacg ccggacttca ccgacatcgt gctgcaggtg gacgacatcc
     1921 ggcacgccat tgccatcgac tacgacccgc tagagggcta tgtctactgg acagatgacg
     1981 aggtgcgggc catccgcagg gcgtacctgg acgggtctgg ggcgcagacg ctggtcaaca
     2041 ccgagatcaa cgaccccgat ggcatcgcgg tcgactgggt ggcccgaaac ctctactgga
     2101 ccgacacggg cacggaccgc atcgaggtga cgcgcctcaa cggcacctcc cgcaagatcc
     2161 tggtgtcgga ggacctggac gagccccgag ccatcgcact gcaccccgtg atgggcctca
     2221 tgtactggac agactgggga gagaacccta aaatcgagtg tgccaacttg gatgggcagg
     2281 agcggcgtgt gctggtcaat gcctccctcg ggtggcccaa cggcctggcc ctggacctgc
     2341 aggaggggaa gctctactgg ggagacgcca agacagacaa gatcgaggtg atcaatgttg
     2401 atgggacgaa gaggcggacc ctcctggagg acaagctccc gcacattttc gggttcacgc
     2461 tgctggggga cttcatctac tggactgact ggcagcgccg cagcatcgag cgggtgcaca
     2521 aggtcaaggc cagccgggac gtcatcattg accagctgcc cgacctgatg gggctcaaag
     2581 ctgtgaatgt ggccaaggtc gtcggaacca acccgtgtgc ggacaggaac ggggggtgca
     2641 gccacctgtg cttcttcaca ccccacgcaa cccggtgtgg ctgccccatc ggcctggagc
     2701 tgctgagtga catgaagacc tgcatcgtgc ctgaggcctt cttggtcttc accagcagag
     2761 ccgccatcca caggatctcc ctcgagacca ataacaacga cgtggccatc ccgctcacgg
     2821 gcgtcaagga ggcctcagcc ctggactttg atgtgtccaa caaccacatc tactggacag
     2881 acgtcagcct gaagaccatc agccgcgcct tcatgaacgg gagctcggtg gagcacgtgg
     2941 tggagtttgg ccttgactac cccgagggca tggccgttga ctggatgggc aagaacctct
     3001 actgggccga cactgggacc aacagaatcg aagtggcgcg gctggacggg cagttccggc
     3061 aagtcctcgt gtggagggac ttggacaacc cgaggtcgct ggccctggat cccaccaagg
     3121 gctacatcta ctggaccgag tggggcggca agccgaggat cgtgcgggcc ttcatggacg
     3181 ggaccaactg catgacgctg gtggacaagg tgggccgggc caacgacctc accattgact
     3241 acgctgacca gcgcctctac tggaccgacc tggacaccaa catgatcgag tcgtccaaca
     3301 tgctgggtca ggagcgggtc gtgattgccg acgatctccc gcacccgttc ggtctgacgc
     3361 agtacagcga ttatatctac tggacagact ggaatctgca cagcattgag cgggccgaca
     3421 agactagcgg ccggaaccgc accctcatcc agggccacct ggacttcgtg atggacatcc
     3481 tggtgttcca ctcctcccgc caggatggcc tcaatgactg tatgcacaac aacgggcagt
     3541 gtgggcagct gtgccttgcc atccccggcg gccaccgctg cggctgcgcc tcacactaca
     3601 ccctggaccc cagcagccgc aactgcagcc cgcccaccac cttcttgctg ttcagccaga
     3661 aatctgccat cagtcggatg atcccggacg accagcacag cccggatctc atcctgcccc
     3721 tgcatggact gaggaacgtc aaagccatcg actatgaccc actggacaag ttcatctact
     3781 gggtggatgg gcgccagaac atcaagcgag ccaaggacga cgggacccag ccctttgttt
     3841 tgacctctct gagccaaggc caaaacccag acaggcagcc ccacgacctc agcatcgaca
     3901 tctacagccg gacactgttc tggacgtgcg aggccaccaa taccatcaac gtccacaggc
     3961 tgagcgggga agccatgggg gtggtgctgc gtggggaccg cgacaagccc agggccatcg
     4021 tcgtcaacgc ggagcgaggg tacctgtact tcaccaacat gcaggaccgg gcagccaaga
     4081 tcgaacgcgc agccctggac ggcaccgagc gcgaggtcct cttcaccacc ggcctcatcc
     4141 gccctgtggc cctggtggta gacaacacac tgggcaagct gttctgggtg gacgcggacc
     4201 tgaagcgcat tgagagctgt gacctgtcag gggccaaccg cctgaccctg gaggacgcca
     4261 acatcgtgca gcctctgggc ctgaccatcc ttggcaagca tctctactgg atcgaccgcc
     4321 agcagcagat gatcgagcgt gtggagaaga ccaccgggga caagcggact cgcatccagg
     4381 gccgtgtcgc ccacctcact ggcatccatg cagtggagga agtcagcctg gaggagttct
     4441 cagcccaccc atgtgcccgt gacaatggtg gctgctccca catctgtatt gccaagggtg
     4501 atgggacacc acggtgctca tgcccagtcc acctcgtgct cctgcagaac ctgctgacct
     4561 gtggagagcc gcccacctgc tccccggacc agtttgcatg tgccacaggg gagatcgact
     4621 gtatccccgg ggcctggcgc tgtgacggct ttcccgagtg cgatgaccag agcgacgagg
     4681 agggctgccc cgtgtgctcc gccgcccagt tcccctgcgc gcggggtcag tgtgtggacc
     4741 tgcgcctgcg ctgcgacggc gaggcagact gtcaggaccg ctcagacgag gcggactgtg
     4801 acgccatctg cctgcccaac cagttccggt gtgcgagcgg ccagtgtgtc ctcatcaaac
     4861 agcagtgcga ctccttcccc gactgtatcg acggctccga cgagctcatg tgtgaaatca
     4921 ccaagccgcc ctcagacgac agcccggccc acagcagtgc catcgggccc gtcattggca
     4981 tcatcctctc tctcttcgtc atgggtggtg tctattttgt gtgccagcgc gtggtgtgcc
     5041 agcgctatgc gggggccaac gggcccttcc cgcacgagta tgtcagcggg accccgcacg
     5101 tgcccctcaa tttcatagcc ccgggcggtt cccagcatgg ccccttcaca ggcatcgcat
     5161 gcggaaagtc catgatgagc tccgtgagcc tgatgggggg ccggggcggg gtgcccctct
     5221 acgaccggaa ccacgtcaca ggggcctcgt ccagcagctc gtccagcacg aaggccacgc
     5281 tgtacccgcc gatcctgaac ccgccgccct ccccggccac ggacccctcc ctgtacaaca
     5341 tggacatgtt ctactcttca aacattccgg ccactgcgag accgtacagg ccctacatca
     5401 ttcgaggaat ggcgcccccg acgacgccct gcagcaccga cgtgtgtgac agcgactaca
     5461 gcgccagccg ctggaaggcc agcaagtact acctggattt gaattcggac tcagacccct
     5521 atccaccccc acccacgccc cacagccagt acctgtcggc ggaggacagc tgcccgccct
     5581 cgcccgccac cgagaggagc tacttccatc tcttcccgcc ccctccgtcc ccctgcacgg
     5641 actcatcctg acaagggttc gatccctacc ggttagtaat gagtttaaac gggggaggct
     5701 aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag
     5761 acagaataaa acgcacgggt gttgggtcgt ttgttcataa acgcggggtt cggtcccagg
     5821 gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc cgcgtttctt
     5881 ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca gccaacgtcg
     5941 gggcggcagg ccctgccata gcagatctgc gcagctgggg ctctaggggg tatccccacg
     6001 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     6061 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     6121 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     6181 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     6241 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     6301 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     6361 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     6421 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6481 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6541 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6601 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6661 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6721 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
     6781 agcttgtata tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat
     6841 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     6901 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     6961 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga
     7021 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     7081 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     7141 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     7201 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     7261 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     7321 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga
     7381 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     7441 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     7501 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     7561 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     7621 gttcttctga gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc
     7681 catcacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
     7741 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     7801 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     7861 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     7921 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     7981 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8041 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8101 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8161 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8221 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8281 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8341 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8401 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8461 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8521 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     8581 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     8641 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     8701 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     8761 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     8821 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     8881 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     8941 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9001 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9061 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9121 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9181 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9241 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9301 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9361 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9421 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9481 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9541 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9601 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     9661 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     9721 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     9781 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     9841 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     9901 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     9961 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10021 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10081 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
    10141 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
    10201 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
    10261 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagg
//
