LOCUS       sequence-225524-       10894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of EGFPN3-hMyoX in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10894)
  TITLE     Strehler unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: EGFPN3-hMyoX
FEATURES             Location/Qualifiers
     source          1..10894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     519..539
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      872..881
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      996..1005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      5768..5777
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      6834..6843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(6840..6842,6843..6845,6846..7559)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6840..6842,6843..6845,6846..7556)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7146..7165)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7493..7514
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    7682..7803
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7719..7738)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7773..7792
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7810..8265)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7947..7968)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8159..8178
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8292..8396
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8398..8755
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8398..8589
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8421..8441)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8606..8741
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8790..9584
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8790..9584
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8844..8863)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9454..9473
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9772..9791)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9816..9863
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10192..10780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10681..10700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc atggataact
      661 tcttcaccga gggaacacgg gtctggctga gagaaaatgg ccagcatttt ccaagtactg
      721 taaattcctg tgcagaaggc atcgtcgtct tccggacaga ctatggtcag gtattcactt
      781 acaagcagag cacaattacc caccagaagg tgactgctat gcaccccacg aacgaggagg
      841 gcgtggatga catggcgtcc ttgacagagc tccatggcgg ctccatcatg tataacttat
      901 tccagcggta taagagaaat caaatatata cctacatcgg ctccatcctg gcctccgtga
      961 acccctacca gcccatcgcc gggctgtacg agcctgccac catggagcag tacagccggc
     1021 gccacctggg cgagctgccc ccgcacatct tcgccatcgc caacgagtgc taccgctgcc
     1081 tgtggaagcg ctacgacaac cagtgcatcc tcatcagtgg tgaaagtggg gcaggtaaaa
     1141 ccgaaagcac taaattgatc ctcaagtttc tgtcagtcat cagtcaacag tctttggaat
     1201 tgtccttaaa ggagaagaca tcctgtgttg aacgagctat tcttgaaagc agccccatca
     1261 tggaagcttt cggcaatgcg aagaccgtgt acaacaacaa ctctagtcgc tttgggaagt
     1321 ttgttcagct gaacatctgt cagaaaggaa atattcaggg cgggagaatt gtagattatt
     1381 tattagaaaa aaaccgagta gtaaggcaaa atcccgggga aaggaattat cacatatttt
     1441 atgcactgct ggcagggctg gaacatgaag aaagagaaga attttattta tctacgccag
     1501 aaaactacca ctacttgaat cagtctggat gtgtagaaga caagacaatc agtgaccagg
     1561 aatcctttag ggaagttatt acggcaatgg acgtgatgca gttcagcaag gaggaagttc
     1621 gggaagtgtc gaggctgctt gctggtatac tgcatcttgg gaacatagaa tttatcactg
     1681 ctggtggggc acaggtttcc ttcaaaacag ctttgggcag atctgcggag ttacttgggc
     1741 tggacccaac acagctcaca gatgctttga cccagagatc aatgttcctc aggggagaag
     1801 agatcctcac gcctctcaat gttcaacagg cagtagacag cagggactcc ctggccatgg
     1861 ctctgtatgc gtgctgcttt gagtgggtaa tcaagaagat caacagcagg atcaaaggca
     1921 atgaggactt caagtctatt ggcatcctcg acatctttgg atttgaaaac tttgaggtta
     1981 atcactttga acagttcaat ataaactatg caaacgagaa acttcaggag tacttcaaca
     2041 agcatatttt ttctttagaa caactagaat atagccggga aggattagtg tgggaagata
     2101 ttgactggat agacaatgga gaatgcctgg acttgattga gaagaaactt ggcctcctag
     2161 cccttatcaa tgaagaaagc cattttcctc aagccacaga cagcacctta ttggagaagc
     2221 tacacagtca gcatgcgaat aaccactttt atgtgaagcc cagagttgca gttaacaatt
     2281 ttggagtgaa gcactatgct ggagaggtgc aatatgatgt ccgaggtatc ttggagaaga
     2341 acagagatac atttcgagat gaccttctca atttgctaag agaaagccga tttgacttta
     2401 tctacgatct ttttgaacat gtttcaagcc gcaacaacca ggataccttg aaatgtggaa
     2461 gcaaacatcg gcggcctaca gtcagctcac agttcaagga ctcactgcat tccttaatgg
     2521 caacgctaag ctcctctaat cctttctttg ttcgctgtat caagccaaac atgcagaaga
     2581 tgccagacca gtttgaccag gcggttgtgc tgaaccagct gcggtactca gggatgctgg
     2641 agactgtgag aatccgcaaa gctgggtatg cggtccgaag accctttcgg gacttttaca
     2701 aaaggtataa agtgctgatg aggaatctgg ctctgcctga ggacgtccga gggaagtgca
     2761 cgagcctgct gcagctctat gatgcctcca acagcgagtg gcagctgggg aagaccaagg
     2821 tctttcttcg agaatccttg gaacagaaac tggagaagcg gagggaagag gaagtgagcc
     2881 acgcggccat ggtgattcgg gcccatgtct tgggcttctt agcacgaaaa caatacagaa
     2941 aggtccttta ttgtgtggtg ataatacaga agaattacag agcattcctt ctgaggagga
     3001 gatttttgca cctgaaaaag gcagccatag ttttccagaa gcaactcaga ggtcagattg
     3061 ctcggagagt ttacagacaa ttgctggcag agaaaaggga gcaagaagaa aagaagaaac
     3121 aggaagagga agaaaagaag aaacgggagg aagaagaaag agaaagagag agagagcgaa
     3181 gagaagccga gctccgcgcc cagcaggaag aagaaacgag gaagcagcaa gaactcgaag
     3241 ccttgcagaa gagccagaag gaagctgaac tgacccgtga actggagaaa cagaaggaaa
     3301 ataagcaggt ggaagagatc ctccgtctgg agaaagaaat cgaggacctg cagcgcatga
     3361 aggagcagca ggagctgtcg ctgaccgagg cttccctgca gaagctgcag gagcggcggg
     3421 accaggagct ccgcaggctg gaggaggaag cgtgcagggc ggcccaggag ttcctcgagt
     3481 ccctcaattt cgacgagatc gacgagtgtg tccggaatat cgagcggtcc ctgtcggtgg
     3541 gaagcgaatt ttccagcgag ctggctgaga gcgcatgcga ggagaagccc aacttcaact
     3601 tcagccagcc ctacccagag gaggaggtcg atgagggctt cgaagccgac gacgacgcct
     3661 tcaaggactc ccccaacccc agcgagcacg gccactcaga ccagcgaaca agtggcatcc
     3721 ggaccagcga tgactcttca gaggaggacc catacatgaa cgacacggtg gtgcccacca
     3781 gccccagtgc ggacagcacg gtgctgctcg ccccatcagt gcaggactcc gggagcctac
     3841 acaactcctc cagcggcgag tccacctact gcatgcccca gaacgctggg gacttgccct
     3901 ccccagacgg cgactacgac tacgaccagg atgactatga ggacggtgcc atcacttccg
     3961 gcagcagcgt gaccttctcc aactcctacg gcagccagtg gtcccccgac taccgctgct
     4021 ctgtggggac ctacaacagc tcgggtgcct accggttcag ctctgagggg gcgcagtcct
     4081 cgtttgaaga tagtgaagag gactttgatt ccaggtttga tacagatgat gagctttcat
     4141 accggcgtga ctctgtgtac agctgtgtca ctctgccgta tttccacagc tttctgtaca
     4201 tgaaaggtgg cctgatgaac tcttggaaac gccgctggtg cgtcctcaag gatgaaacct
     4261 tcttgtggtt ccgctccaag caggaggccc tcaagcaagg ctggctccac aaaaaagggg
     4321 ggggctcctc cacgctgtcc aggagaaatt ggaagaagcg ctggtttgtc ctccgccagt
     4381 ccaagctgat gtactttgaa aacgacagcg aggagaagct caagggcacc gtagaagtgc
     4441 gaacggcaaa agagatcata gataacacca ccaaggagaa tgggatcgac atcattatgg
     4501 ccgataggac tttccacctg attgcagagt ccccagaaga tgccagccag tggttcagcg
     4561 tgctgagtca ggtccacgcg tccacggacc aggagatcca ggagatgcat gatgagcagg
     4621 caaacccaca gaatgctgtg ggcaccttgg atgtggggct gattgattct gtgtgtgcct
     4681 ctgacagccc tgatagaccc aactcgtttg tgatcatcac ggccaaccgg gtgctgcact
     4741 gcaacgccga cacgccggag gagatgcacc actggataac cctgctgcag aggtccaaag
     4801 gggacaccag agtggagggc caggaattca tcgtgagagg atggttgcac aaagaggtga
     4861 agaacagtcc gaagatgtct tcactgaaac tgaagaaacg gtggtttgta ctcacccaca
     4921 attccctgga ttactacaag agttcagaga agaacgcgct caaactgggg accctggtcc
     4981 tcaacagcct ctgctctgtc gtccccccag atgagaagat attcaaagag acaggctact
     5041 ggaacgtcac cgtgtacggg cgcaagcact gttaccggct ctacaccaag ctgctcaacg
     5101 aggccacccg gtggtccagt gccattcaaa acgtgactga caccaaggcc ccgatcgaca
     5161 cccccaccca gcagctgatt caagatatca aggagaactg cctgaactcg gatgtggtgg
     5221 aacagattta caagcggaac ccgatccttc gatacaccca tcaccccttg cactccccgc
     5281 tcctgcccct tccgtatggg gacataaatc tcaacttgct caaagacaaa ggctatacca
     5341 cccttcagga tgaggccatc aagatattca attccctgca gcaactggag tccatgtctg
     5401 acccaattcc aataatccag ggcatcctac agacagggca tgacctgcga cctctgcggg
     5461 acgagctgta ctgccagctt atcaaacaga ccaacaaagt gccccacccc ggcagtgtgg
     5521 gcaacctgta cagctggcag atcctgacat gcctgagctg caccttcctg ccgagtcgag
     5581 ggattctcaa gtatctcaag ttccatctga aaaggatacg ggaacagttt ccaggaaccg
     5641 agatggaaaa atacgctctc ttcacttacg aatctcttaa gaaaaccaaa tgccgagagt
     5701 ttgtgccttc ccgagatgaa atagaagctc tgatccacag gcaggaaatg acatccacgg
     5761 tctattgcca tggcggcggc tcctgcaaga tcaccatcaa ctcccacacc actgctgggg
     5821 aggtggtgga gaagctgatc cgaggcctgg ccatggagga cagcaggaac atgtttgctt
     5881 tgtttgaata caacggccac gtcgacaaag ccattgaaag tcgaaccgtc gtagctgatg
     5941 tcttagccaa gtttgaaaag ctggctgcca catccgaggt tggggacctg ccatggaaat
     6001 tctacttcaa actttactgc ttcctggaca cagacaacgt gccaaaagac agtgtggagt
     6061 ttgcatttat gtttgaacag gcccacgaag cggttatcca tggccaccat ccagccccgg
     6121 aagaaaacct ccaggttctt gctgccctgc gactccagta tctgcagggg gattatactc
     6181 tgcacgctgc catcccacct ctcgaagagg tttattccct gcagagactc aaggcccgca
     6241 tcagccagtc aaccaaaacc ttcacccctt gtgaacggct ggagaagagg cggacgagct
     6301 tcctagaggg gaccctgagg cggagcttcc ggacaggatc cgtggtccgg cagaaggtcg
     6361 aggaggagca gatgctggac atgtggatta aggaagaagt ctcctctgct cgagccagta
     6421 tcattgacaa gtggaggaaa tttcagggaa tgaaccagga acaggccatg gccaagtaca
     6481 tggccttgat caaggagtgg cctggctatg gctcgacgct gtttgatgtg gagtgcaagg
     6541 aaggtggctt ccctcaggaa ctctggttgg gtgtcagcgc ggacgccgtc tccgtctaca
     6601 agcgtggaga gggaagacca ctggaagtct tccagtatga acacatcctc tcttttgggg
     6661 cacccctggc gaatacgtat aagatcgtgg tcgatgagag ggagctgctc tttgaaacca
     6721 gtgaggtggt ggatgtggcc aagctcatga aagcctacat cagcatgatc gtgaagaagc
     6781 gctacagcac gacacgctcc gccagcagcc agggcagctc caggagatcc atcgccacca
     6841 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     6901 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     6961 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     7021 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     7081 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     7141 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     7201 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     7261 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     7321 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     7381 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     7441 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     7501 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaaa
     7561 gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     7621 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     7681 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7741 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7801 ttaaggcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7861 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7921 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7981 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     8041 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     8101 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     8161 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     8221 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc
     8281 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     8341 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg
     8401 aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8461 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     8521 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8581 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     8641 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     8701 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg
     8761 atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     8821 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     8881 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     8941 ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg
     9001 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     9061 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     9121 agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct
     9181 gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg
     9241 gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt
     9301 tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg
     9361 cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc
     9421 ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag
     9481 agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt
     9541 cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt
     9601 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     9661 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     9721 gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg
     9781 gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac
     9841 gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt
     9901 cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc
     9961 ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc
    10021 tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt
    10081 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    10141 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    10201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    10261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    10321 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    10381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    10441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
    10501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
    10561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
    10621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
    10681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
    10741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
    10801 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
    10861 tgattctgtg gataaccgta ttaccgccat gcat
//
