LOCUS       sequence-225535-         628 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  For expression in E.coli and affinity purification of N-terminally 
            His tagged NQO1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Tsvetkov P, Asher G, Reiss V, Shaul Y, Sachs L, Lotem J
  TITLE     Inhibition of NAD(P)H:quinone oxidoreductase 1 activity and 
            induction of p53 degradation by the natural phenolic compound 
            curcumin.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Apr 12;102(15):5535-40. doi: 
            10.1073/pnas.0501828102. Epub 2005 Apr 4.
  PUBMED    15809436
REFERENCE   2  (bases 1 to 628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0501828102"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2005-04-12- 12"; volume: "102"; issue: "15"; pages: "5535-40"
COMMENT     Sequence Label: pET28-His-TEV-NQO1
FEATURES             Location/Qualifiers
     source          1..628
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..46
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 tcatcatcat catcactccg cgggtgaaaa cctgtacttc cagggtgtcg gcagaagagc
       61 actgatcgta ctggctcact cagagaggac ctccttcaac tatgccatga aggaggctgc
      121 tgcagcggct ttgaagaaga aaggatggga ggtggtggag tcggacctct atgccatgaa
      181 cttcaatccc atcatttcca gaaaggacat cacaggtaaa ctgaaggacc ctgcgaactt
      241 tcagtatcct gccgagtctg ttctggctta taaagaaggc catctgagcc cagatattgt
      301 ggctgaacaa aagaagctgg aagccgcaga ccttgtgata ttccagttcc ccctgcagtg
      361 gtttggagtc cctgccattc tgaaaggctg gtttgagcga gtgttcatag gagagtttgc
      421 ttacacttac gctgccatgt atgacaaagg acccttccgg agtaagaagg cagtgctttc
      481 catcaccact ggtggcagtg gctccatgta ctctctgcaa gggatccacg gggacatgaa
      541 tgtcattctc tggccaattc agagtggcat tctgcatttc tgtggcttcc aagtcttaga
      601 acctcaactg acatatagca ttgggcac
//
