LOCUS       sequence-225590-       10556 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLAG-tagged LINXa4-Bre1 with RVH hinge-loop mutations.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10556)
  AUTHORS   Lerner AM, Yumerefendi H, Goudy OJ, Strahl BD, Kuhlman B
  TITLE     Engineering Improved Photoswitches for the Control of 
            Nucleocytoplasmic Distribution.
  JOURNAL   ACS Synth Biol. 2018 Nov 29. doi: 10.1021/acssynbio.8b00368.
  PUBMED    30441907
REFERENCE   2  (bases 1 to 10556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2018 Nov 29. doi: 10.1021/acssynbio.8b00368."
COMMENT     Sequence Label: pNIA-CEN-FLAG-LINXa4(RVH)-Bre1
FEATURES             Location/Qualifiers
     source          1..10556
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    52..73
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(88..93,94..111,112..118)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    126..142
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     131..153
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     150..166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     185..205
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        187..205
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        974..1678
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     CDS             1732..1755
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1807..2235
                     /codon_start=1
                     /gene="NPH1 from Avena sativa"
                     /product="light, oxygen, or voltage domain from phototropin
                     1 of Avena sativa (Herrou and Crosson, 2011)"
                     /label=LOV2
                     /note="photoswitch that undergoes a light-inducible 
                     conformational change"
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQ
                     YFIGVQLDGTERVHDAAEREGVMLIKKTAENIDEAAKEL"
     terminator      4399..4646
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4415..4433)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4664..4683)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4665..4683)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4693..4710)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4693..4709)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4702..4724)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(4845..4862)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5016..5604)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5096..5115)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5775..6566,6567..6635))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6398..6417
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6636..6740)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6808..6826
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6846..7251
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7252..8346
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     misc_feature    8708..9870
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      8708..9545
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
ORIGIN
        1 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
       61 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
      121 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
      181 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctcgccggga tcgaagaaat
      241 gatggtaaat gaaataggaa atcaaggagc atgaaggcaa aagacaaata taagggtcga
      301 acgaaaaata aagtgaaaag tgttgatatg atgtatttgg ctttgcggcg ccgaaaaaac
      361 gagtttacgc aattgcacaa tcatgctgac tctgtggcgg acccgcgctc ttgccggccc
      421 ggcgataacg ctgggcgtga ggctgtgccc ggcggagttt tttgcgcctg cattttccaa
      481 ggtttaccct gcgctaaggg gcgagattgg agaagcaata agaatgccgg ttggggttgc
      541 gatgatgacg accacgacaa ctggtgtcat tatttaagtt gccgaaagaa cctgagtgca
      601 tttgcaacat gagtatacta gaagaatgag ccaagacttg cgagacgcga gtttgccggt
      661 ggtgcgaaca atagagcgac catgaccttg aaggtgagac gcgcataacc gctagagtac
      721 tttgaagagg aaacagcaat agggttgcta ccagtataaa tagacaggta catacaacac
      781 tggaaatggt tgtctgtttg agtacgcttt caattcattt gggtgtgcac tttattatgt
      841 tacaatatgg aagggaactt tacacttctc ctatgcacat atattaatta aagtccaatg
      901 ctagtagaga aggggggtaa cacccctccg cgctcttttc cgattttttt ctaaaccgtg
      961 gaatatttcg gatatccttt tgttgtttcc gggtgtacaa tatggacttc ctcttttctg
     1021 gcaaccaaac ccatacatcg ggattcctat aataccttcg ttggtctccc taacatgtag
     1081 gtggcggagg ggagatatac aatagaacag ataccagaca agacataatg ggctaaacaa
     1141 gactacacca attacactgc ctcattgatg gtggtacata acgaactaat actgtagccc
     1201 tagacttgat agccatcatc atatcgaagt ttcactaccc tttttccatt tgccatctat
     1261 tgaagtaata ataggcgcat gcaacttctt ttcttttttt ttcttttctc tctcccccgt
     1321 tgttgtctca ccatatccgc aatgacaaaa aaatgatgga agacactaaa ggaaaaaatt
     1381 aacgacaaag acagcaccaa cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa
     1441 gcacacgaaa ctttttcctt ccttcattca cgcacactac tctctaatga gcaacggtat
     1501 acggccttcc ttccagttac ttgaatttga aataaaaaaa agtttgctgt cttgctatca
     1561 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt
     1621 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcc
     1681 aagcttgaat taattccggg cggaatgaaa gcgttaacgt ctggcggccg cgattacaag
     1741 gacgacgatg acaagtctag agccgccgcc aagagggtga agctggacgg ttctggaagt
     1801 gggtccttgg ctactacact tgaacgtatt gagaagaact ttgtcattac tgacccaaga
     1861 ttgccagata atcccattat attcgcgtcc gatagtttct tgcagttgac agaatatagc
     1921 cgtgaagaaa ttttgggaag aaactgcagg tttctacaag gtcctgaaac tgatcgcgcg
     1981 acagtgagaa aaattagaga tgccatagat aaccaaacag aggtcactgt tcagctgatt
     2041 aattatacaa agagtggtaa aaagttctgg aacctctttc acttgcagcc tatgcgagat
     2101 cagaagggag atgtccagta ctttattggg gttcagttgg atggaactga gcgagtccac
     2161 gatgctgccg agagagaggg agtcatgctg attaagaaaa ctgcagaaaa tattgatgaa
     2221 gcggcaaaag agctggccgg gctggatctg gggtctggaa gtgggtcggg gtcccgggga
     2281 acggccgagc ctgctactgc cgctatcgca ctagaattaa gcgatcctag tgaaccgctt
     2341 actcaatctg atgtgatagc gtttcaaaaa gaagctttgt ttagatgtat aaatcgtcga
     2401 agagtcgact ttgaagcctt gcgaaagcaa tatgaacttt caagaaggga gtgcatagat
     2461 gtttcccgca agttggcaaa tattatggcg ctgatagtaa ctttagctag gtttatcgaa
     2521 acattctgca cagacgccaa cgagaagcaa ctatgtaggg aaatagccca aggcgatgag
     2581 acgttgatag ttcagcgcag tgattctttc atgaagctac tgactaaata tgggaagcca
     2641 aacacgacag atagtaatac taatagtaat gcttcggatc atattcaaga attaacaacg
     2701 gagttaaaaa acttgcggaa aagtaaagag gagctctttt atgaaaattc gcaattgaca
     2761 gaggagattt ctgctttgaa ggaatattat acaaatataa ttcgcaagta tgatagagat
     2821 gaatccttta ctataaaacg tgtttttaag gaagataaga ctgatgctgt aaaggaatta
     2881 cgggaagatg aaaaagaatc taatgaaaac aatataaaat ctggaaataa agactcctct
     2941 gcaattaatg gtgataatac atccaaaaaa tccgagaaag gtgatgaact tgtgcaggcg
     3001 gaggatgaac gaaaagaaga tgctgagaat gaaaaattag aattggatct gaaattttct
     3061 gatttacggg cagagataaa ctccttgagt tccactataa aagaccttga aaatatcaga
     3121 aggggaaacg aggaagagct catcaaaaca cgttctgagg tatccaattt aaaaaagcaa
     3181 cagatagcag cagcagacca agacccagat ttcaagtcat atgaccatga gtccctgtta
     3241 gcaaaaattc aacatttgac tgaacaaaac gcagaattat ctgagatcaa tagttccttt
     3301 ttatcaaaat ttcaagtttt agccaaagag aaagaaatat atacgaataa ggtccgagaa
     3361 gaattccaga aaagtctcga ctccctagta gaaatgaaca gttcattgga aaaggacgtt
     3421 gtaagaataa gaacggctcg tgatgattta ctaagcaaaa tcgccatttt ggaagctgaa
     3481 aaatccaaga ctgaagtgtt gagcgattta caacatgcaa ttgatatttt gaagaagcaa
     3541 tggacaaaaa ttgatcaaag atcaaacgat acaaaatcct cgtcgactca agatgcatta
     3601 ataaaggaaa ttcaagactt agagaagggt tttagagagt tatccgactt aactcataaa
     3661 aagtactctg agattattaa tcacgaatct gttatatcca agcttaccgt agaaaaaact
     3721 aaggctgatc agaaatattt tgccgcgatg aggtccaaag attcaatttt aattgaaatc
     3781 aaaactcttt ctaaaagcct cagtaaatca aatgagctta ttctacagtt aaaagactct
     3841 gatagattgc tacagcaaaa aataggaaat ttacataaac aactggatct ttctcaaaat
     3901 aatgaacgca ggttaattga ttccagtaaa acagagacac taaaaataat tgatttaaat
     3961 aatacatcca ctaaactgaa aaggtcatta gaaaagctac aagaagaaag taacaaaagc
     4021 attgcagata tgacgcatct agaaactaaa ttaaatgata cagaaataga attgaagcac
     4081 ttcaagcaga aggcatctca tctagaatcc aagtgtgaaa aactacatga tacattgttc
     4141 cgtggtaaca acaaaaataa gggctcgagc gatgaagcac tcgtagaaga actagcaaat
     4201 ttccgtacac ttgtttattg ttccctgtgt tctaaaaatt ggaaaaacat ggctattaaa
     4261 acctgtggcc atgtcttttg tgagaattgc tgtaaggaac gattggcagc gagaatgaga
     4321 aaatgcccta catgcaacaa agcattcagt tccaatgatt tattgacagt gcacttgtaa
     4381 ggatccgtcg acctcgagtc atgtaattag ttatgtcacg cttacattca cgccctcccc
     4441 ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt
     4501 atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt
     4561 ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt
     4621 tgggacgctc gaaggcttta atttgcggcc ggtacccaat tcgccctata gtgagtcgta
     4681 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     4741 ccaacttaat cgccttgcag cacatccccc tttcgccagc tgcattaatg aatcggccaa
     4801 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     4861 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     4921 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     4981 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     5041 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     5101 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     5161 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     5221 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     5281 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     5341 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     5401 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     5461 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     5521 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     5581 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     5641 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     5701 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     5761 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     5821 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     5881 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     5941 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     6001 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     6061 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     6121 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     6181 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     6241 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     6301 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     6361 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     6421 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     6481 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     6541 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     6601 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     6661 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     6721 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     6781 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa
     6841 gaattaactg tgggaatact caggtatcgt aagatgcaag agttcgaatc tcttagcaac
     6901 cattattttt ttcctcaaca taacgagaac acacaggggc gctatcgcac agaatcaaat
     6961 tcgatgactg gaaatttttt gttaatttca gaggtcgcct gacgcatata cctttttcaa
     7021 ctgaaaaatt gggagaaaaa ggaaaggtga gagcgccgga accggctttt catatagaat
     7081 agagaagcgt tcatgactaa atgcttgcat cacaatactt gaagttgaca atattattta
     7141 aggacctatt gttttttcca ataggtggtt agcaatcgtc ttactttcta acttttctta
     7201 ccttttacat ttcagcaata tatatatata tttcaaggat ataccattct aatgtctgcc
     7261 cctaagaaga tcgtcgtttt gccaggtgac cacgttggtc aagaaatcac agccgaagcc
     7321 attaaggttc ttaaagctat ttctgatgtt cgttccaatg tcaagttcga tttcgaaaat
     7381 catttaattg gtggtgctgc tatcgatgct acaggtgttc cacttccaga tgaggcgctg
     7441 gaagcctcca agaaggctga tgccgttttg ttaggtgctg tgggtggtcc taaatggggt
     7501 acaggtagtg ttagacctga acaaggttta ctaaaaatcc gtaaagaact tcaattgtac
     7561 gccaacttaa gaccatgtaa ctttgcatcc gactctcttt tagacttatc tccaatcaag
     7621 ccacaatttg ctaaaggtac tgacttcgtt gttgtcagag aattagtggg aggtatttac
     7681 tttggtaaga gaaaggaaga cgatggtgat ggtgtcgctt gggatagtga acaatacacc
     7741 gttccagaag tgcaaagaat cacaagaatg gccgctttca tggccctaca acatgagcca
     7801 ccattgccta tttggtcctt ggataaagct aatgttttgg cctcttcaag attatggaga
     7861 aaaactgtgg aggaaaccat caagaacgaa tttcctacat tgaaggttca acatcaattg
     7921 attgattctg ccgccatgat cctagttaag aacccaaccc acctaaatgg tattataatc
     7981 accagcaaca tgtttggtga tatcatctcc gatgaagcct ccgttatccc aggttccttg
     8041 ggtttgttgc catctgcgtc cttggcctct ttgccagaca agaacaccgc atttggtttg
     8101 tacgaaccat gccacggttc tgctccagat ttgccaaaga ataaggtcaa ccctatcgcc
     8161 actatcttgt ctgctgcaat gatgttgaaa ttgtcattga acttgcctga agaaggtaag
     8221 gccattgaag atgcagttaa aaaggttttg gatgcaggta tcagaactgg tgatttaggt
     8281 ggttccaaca gtaccaccga agtcggtgat gctgtcgccg aagaagttaa gaaaatcctt
     8341 gcttaaaaag attctctttt tttatgatat ttgtacataa actttataaa tgaaattcat
     8401 aatagaaacg acacgaaatt acaaaatgga atatgttcat agggtagaat taattctcat
     8461 gtttgacagc ttatcatcga tcgtccaact gcatggagat gagtcgtggc aagaatacca
     8521 agagttcctc ggtttgccag ttattaaaag actcgtattt ccaaaagact gcaacatact
     8581 actcagtgca gcttcacaga aacctcattc gtttattccc ttgtttgatt cagaagcagg
     8641 tgggacaggt gaacttttgg attggaactc gatttctgac tgggttggaa ggcaagagag
     8701 ccccgagagc ttacatttta tgttagctgg tggactgacg ccagaaaatg ttggtgatgc
     8761 gcttagatta aatggcgtta ttggtgttga tgtaagcgga ggtgtggaga caaatggtgt
     8821 aaaagactct aacaaaatag caaatttcgt caaaaatgct aagaaatagg ttattactga
     8881 gtagtattta tttaagtatt gtttgtgcac ttgcctgcaa gccttttgaa aagcaagcat
     8941 aaaagatcta aacataaaat ctgtaaaata acaagatgta aagataatgc taaatcattt
     9001 ggctttttga ttgattgtac aggaaaatat acatcgcagg gggttgactt ttaccatttc
     9061 accgcaatgg aatcaaactt gttgaagaga atgttcacag gcgcatacgc tacaatgacc
     9121 cgattcttgc tagccttttc tcggtcttgc aaacaaccgc cggcagctta gtatataaat
     9181 acacatgtac atacctctct ccgtatcctc gtaatcattt tcttgtattt atcgtctttt
     9241 cgctgtaaaa actttatcac acttatctca aatacactta ttaaccgctt ttactattat
     9301 cttctacgct gacagtaata tcaaacagtg acacatatta aacacagtgg tttctttgca
     9361 taaacaccat cagcctcaag tcgtcaagta aagatttcgt gttcatgcag atagataaca
     9421 atctatatgt tgataattag cgttgcctca tcaatgcgag atccgtttaa ccggacccta
     9481 gtgcacttac cccacgttcg gtccactgtg tgccgaacat gctccttcac tattttaaca
     9541 tgtggaatta attctcatgt ttgacagctt atcatcgaac tctaagaggt gatacttatt
     9601 tactgtaaaa ctgtgacgat aaaaccggaa ggaagaataa gaaaactcga actgatctat
     9661 aatgcctatt ttctgtaaag agtttaagct atgaaagcct cggcattttg gccgctccta
     9721 ggtagtgctt tttttccaag gacaaaacag tttctttttc ttgagcaggt tttatgtttc
     9781 ggtaatcata aacaataaat aaattatttc atttatgttt aaaaataaaa aataaaaaag
     9841 tattttaaat ttttaaaaaa gttgattata agcatgtgac cttttgcaag caattaaatt
     9901 ttgcaatttg tgatttaggc aaaagttact atttctggct cgtgtaatat atgtatgcta
     9961 atgtgaactt ttacaaagtc gatatggact tagtcaaaag aaattttctt aaaaatatat
    10021 agcactagcc aatttagcac ttctttatga gatatattat agactttatt aagccagatt
    10081 tgtgtattat atgtatttac ccggcgaatc atggacatac attctgaaat aggtaatatt
    10141 ctctatggtg agacagcata gataacctag gatacaagtt aaaagctagt actgttttgc
    10201 agtaattttt ttctttttta taagaatgtt accacctaaa taagttataa agtcaatagt
    10261 taagtttgat atttgattgt aaaataccgt aatatatttg catgatcaaa aggctcaatg
    10321 ttgactagcc agcatgtcaa ccactatatt gatcaccgat attaggactt ccacaccaac
    10381 tagtaatatg acaataaatt caagatattc ttcatgagaa tggcccagcg attatgcggt
    10441 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca
    10501 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccag
//
