LOCUS       sequence-225595-         741 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-terminus of soybean mosaic virus protease (SbMVp) fused 
            to FKBP-rapamycin binding (FRB) domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Fink T, Lonzaric J, Praznik A, Plaper T, Merljak E, Leben K, Jerala 
            N, Lebar T, Strmsek Z, Lapenta F, Bencina M, Jerala R
  TITLE     Design of fast proteolysis-based signaling and logic circuits in 
            mammalian cells.
  JOURNAL   Nat Chem Biol. 2019 Feb;15(2):115-122. doi: 
            10.1038/s41589-018-0181-6. Epub 2018 Dec 10.
  PUBMED    30531965
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-018-0181-6"; journalName: "Nat Chem Biol"; date: 
            "2019-02"; volume: "15"; issue: "2"; pages: "115-122"
COMMENT     Sequence Label: FRB_nSbMVp
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..63
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             64..342
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
ORIGIN
        1 gcgcgaattc gcggccgctc tagagccacc atggagcaga agctgatcag cgaggaggat
       61 ctgatcctct ggcatgagat gtggcatgaa ggcctggaag aggcatctcg tttgtacttt
      121 ggggaaagga acgtgaaagg catgtttgag gtgctggagc ccttgcatgc tatgatggaa
      181 cggggccccc agactctgaa ggaaacatcc tttaatcagg cctatggtcg agatttaatg
      241 gaggcccaag agtggtgcag gaagtacatg aaatcaggga atgtcaagga cctcctccaa
      301 gcctgggacc tctattatca tgtgttccga cgaatctcaa agggatccgg aagtagcaag
      361 agcgtgtaca agggcctgcg cgactacagc ggcatcagca ccctgatctg ccagctgacc
      421 aacagcagcg acggccacaa ggagaccatg ttcggcgtgg gctacggcag cttcatcatc
      481 accaacggcc acctgttccg ccgcaacaac ggcatgctga ccgtgaagac ctggcacggc
      541 gagttcgtga tccacaacac cacccagctg aagatccact tcatccaggg ccgcgacgtg
      601 atcctgatcc gcatgcccaa ggacttcccc cccttcggca agcgcaacct gttccgccag
      661 cccaagcgcg aggagcgcgt gtgcatggtg ggcaccaact tccaggagta aactagtgcg
      721 gccgcctgca gctcgaggcg c
//
