LOCUS       sequence-225609-       13021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of a fungal biosynthetic gene cluster.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13021)
  AUTHORS   Harvey CJB, Tang M, Schlecht U, Horecka J, Fischer CR, Lin HC, Li J,
            Naughton B, Cherry J, Miranda M, Li YF, Chu AM, Hennessy JR, Vandova
            GA, Inglis D, Aiyar RS, Steinmetz LM, Davis RW, Medema MH, Sattely 
            E, Khosla C, St Onge RP, Tang Y, Hillenmeyer ME
  TITLE     HEx: A heterologous expression platform for the discovery of fungal 
            natural products.
  JOURNAL   Sci Adv. 2018 Apr 11;4(4):eaar5459. doi: 10.1126/sciadv.aar5459. 
            eCollection 2018 Apr.
  PUBMED    29651464
REFERENCE   2  (bases 1 to 13021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2018-04-11"; volume: "4(4):eaar5459. doi"; pages: " 
            10.1126/sciadv.aar5459. eCollection 2018 Apr"
COMMENT     Sequence Label: pCHKU38.1-2.2
FEATURES             Location/Qualifiers
     source          1..13021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             252..275
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7193..7213)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7193..7211)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(7550..8138)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7630..7649)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8309..9100,9101..9169))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8932..8951
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9170..9274)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9301..10643
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10264..10311)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     10684..10702
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        10997..11404
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             11405..12499
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     primer_bind     complement(12746..12762)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12746..12762)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gagaatgaaa aaaaaaaaaa aaaaaaggca gaggagagca tagaaatggg gttcactttt
       61 tggtaaagct atagcatgcc tatcacatat aaatagagtg ccagtagcga cttttttcac
      121 actcgaaata ctcttactac tgctctcttg ttgtttttat cacttcttgt ttcttcttgg
      181 taaatagaat atcaagctac aaaaagcata caatcaacta tcaactatta actatatcgt
      241 aatacacaat ggattataag gatgatgatg ataagactag tatgacaaaa gctatgcggg
      301 tcttcctctt tggagatcta aatgtctcct tcgaggagag cctcagagag ttgctctaca
      361 ccaaggacat gccagtcttg aaatctttcc ttgaaaaggt ggcttttgcg cttcgtgagg
      421 agatttcccg catgtcctct tcctggcagg cgttaatgcc acgttttact accttaatcg
      481 atctgctccc tcagatcaat ggcaagatag gaagtccggc gcttagattt gctttgctct
      541 gtgtggcgga gctaggactg ttcattcgct tccatgagcg aaggtctatt ccatatccta
      601 ccggggacaa ctcgtgtctc ttgggtatct gcactggatc ttttgctgca gctgcagtcg
      661 ccgtatcccg tggccttacg gacctcgttc ccgctggcgt cgaggcgact cttgtagccc
      721 tacgtacagc cacactctcg ctgcaatgtc gctttgacat gtgcaacatg acagagacag
      781 gctcatcctg ctggtccgct attgtcaacc tcacggaaga ggacgctact aagcttgttg
      841 atgctttctg tgaagctcat ccgcgagtca gtaggcccta tctcagtgcg gtgtccccaa
      901 caaatgtgac catcagtgcg aatcctcaag ttctcgacga cttcttggcc ttcgccaaag
      961 tgcaatttcg aaagctctcc atcgagtctc cataccatgc gccacagcta tttccggaag
     1021 ttgtttgcct ttctacggtc aaaaaatatc acgattctca gctcgaagca cgccagttgc
     1081 atgttccgct cctgcttcct tctggagcag aatgcgagtc tcgaagcttc gaagatgtgt
     1141 tgaaggcgag cactcttcac acactccatg agaaagtacg atgggatcgt gttatcgaaa
     1201 ctgtgtgcac tttactgcag cagcgtaagg ctagtgcgtg cgaaatcatt cctgtgctgt
     1261 ccaatgcgcc gcagctattg ttcacgcaca ttcctcggaa gtgtgacatc caaactgtcg
     1321 tcagcgacct agagcgatgc gaccaaggat cggcttggag caatccaacc ggcaagtttg
     1381 agcagtccaa gatcgcgatc atcggctttt cgggtcggta tcctgactct tccagcaacg
     1441 acgagttctg ggagctgcta cgtgctggac gcgatgtgca caagagaatc cccgaggata
     1501 ggttcgactg ggaggcacac ttcgatccca cgggaaagaa gagaaatacc agcagggtcc
     1561 agtacgggtg cttcatcaaa gagcctggcg ttttcgacac tcgatttttc aacatgtctc
     1621 cacgcgaggc tgctagtacc gatcctgcgc agcgcctgtt gatcacgtct acgtatgaag
     1681 cgcttgagat ggcgggcttc gtgccgaatc gtacgccgtc aagccaggtg gaccgcgttg
     1741 gggttttcat gggcacaaca agcgacgact ggcgcgaggt caacagcgga caagatgtcg
     1801 acacgtactt catcccgggc gggaaccgcg ccttcatccc cggcagaatc agttatttct
     1861 tcaagttctc cgggccgagc ctcagcatcg atacggcgtg ctcttccagc ttctccgcca
     1921 tccacgcagc ctgcgcgtac ttgtggaagg gcgactgtga cacggcgatc gcgggcggca
     1981 ccaacgtcct gaccaatccg gacaacttcg ccgggcttga ccgaggccac tttctgtcta
     2041 caacgggaaa ctgtaaaccg ttcgacgacg ctgcggatgg ctactgccgt gcagacgcgg
     2101 ttggctgcgt catcctcaag cgatacgagg acgccgtcgc cgacaacgac cccgtctacg
     2161 gcctgatcgc aggagcgcac acaaatcatt gcggccaggc cgaatccatc acgcgtcctc
     2221 acgagggcga tcaggcgtcc gtgtttaatc gcgtcttgag atacaccaac caagatcctc
     2281 gggacgtcag ctacgttgag atgcacggaa caggcacgca agctggggat gccaccgaga
     2341 tgaactccgt cctgtcatgc tttgtcagag gcaagcaacg gacagcagag tatccgttgt
     2401 acattggctc tgcgaaggcc aacattgggc acgcagaatc tgcatctggc gtctcctcgc
     2461 tgatcaaggt gttgctaatg atgaagcaca acgaaatccc gccgcactgc ggaatcaagg
     2521 ggaagatcaa ccacaattac ccagatctca aggcgctgaa tgtccacatt gctttcaaac
     2581 caacaccgtg gacacgcgag ggctgcgtcg gtggcaaacg tattgcattt ttgaacaatt
     2641 tcagcgccgc aggcggcaat acggctcttt tgctcgaaga cggtcccgtt cgcgcaaccc
     2701 cagcaaaaga catttcaagg tcaatttacc ctgtggcagt ttctgcgaag agtgcgaaat
     2761 ctctcgagcg taatgtgaaa gcaatgattg catttcttga ggcgaatcct gacactgctc
     2821 tccctagcct ctcatatacc accacagcgc gacgcattca tcacaacttc agaaccatgg
     2881 tttctggaac agacttaaag tcaataaagc tcgagcttga caagatcctg tcaagtcttc
     2941 cagagaagcc cattcccaat gctgctttgc tgccgaagat tgcatttgct tttactggtc
     3001 agggttcctt gtacgtggga gtcacgaagg agcttttcga tgccgacgaa gtgttcagga
     3061 gcgaagtgct gcgcttggat atcattgctc aacagcatgg actaccaagc ttcattccac
     3121 tcctcgatgg aagtgcatct gcgaggcaac ttgaacaacc tactatggcg catgttggtc
     3181 acacggcgct gcagattgcc ctaacgaaca tgtggtctag gtttggaatt tctcctgctc
     3241 tggtcatcgg ccatagcctt ggcgaatacg ccgcacttta tgctgcagga gtgctgacgg
     3301 cctctgatgc gatttatctt gttgggtccc gcgcgcgtct gcttgaacaa tcctgtactc
     3361 ctcgctcgca tgccatgatt tcagtcaagg cttccatggc tgcagttgag ccaatagtcc
     3421 aaagtgggtc atcctgcgag attgcatgtg taaatcagcc tgatgccacc gttttgagcg
     3481 gtccgcggga agaaatagat tgcattatga aagagctcga aacactgggg cataaatgca
     3541 tcaaacttga aattccatat gccttccatt cggcacaggt tgacccgatt ctggagcctc
     3601 tgggactttg tgaaagagct gtacaatttt caaatccgaa aattcctttc ctgtcaccaa
     3661 tgatggcagc tgtaatcaca gaggtggaac ctttcaagga tgcatatctg actaaagcat
     3721 gtcgtaaacc agtcaatttc catgctgctc ttcaagctgc gcaggaatta ggaatagtgg
     3781 gtgacaagac tttatggctt gaaatcggac ctcatccttt gtgcgcggga atgatcaaag
     3841 cgactcttgg ctctgactca gtcacaatgc caactctgac aaaacgccag acctctttgg
     3901 cctctgtggt tgttgcttta caagatctct acatacgagg aattgctgtt gactggaacg
     3961 agtattatcg acaagacgct acttcgcttc aagttattga gcttccacag tacaagtggg
     4021 atcttaagaa ctactggatt caatataaaa acaacttctg tctaactaag ggcgacagca
     4081 ttgagacaaa ggatctttca cgctgtaccg taccgaccgt cgaaaaaacg attatcacgc
     4141 ctgctgttca acacattatt gaaatcagta aggaaaaggc ctccattgtt actgagacag
     4201 acatcagcca ttctgcactt tccaacgtga ttcaaggcca caaggtcaat ggaattgcat
     4261 tgtgtccgtc atctctctac gcagacatgg ccatgactgt ttgtgaacag ttgtgcaacg
     4321 cctgcgagat acgcatcgag gaaagcagct tcgaggtcgg tagtatgaac gtcaaaaagc
     4381 ctcttatcgc agatggatca actcaattat tgcgcgtatc cgcaacagct gatttcgatt
     4441 cacgggaaat tgatttgtct ttttacagtg tttccgcgag tggtactaaa ttagctgacc
     4501 acgcaacttg taaagctcgt atcaggagca agggctctcg cgatgactgg caaaggatgt
     4561 ctttcctgat caaacacaga ctggaagctc ttgaagccag tgttgatcat ggcaccgcga
     4621 ataagatgaa ccggggtatt gtttacaagc tcttcagcgc tttagtcgac tacagtcaag
     4681 gttatcgcgg catggaggag gtaactcttg acagcagtgc acttgaagct acggccaagg
     4741 ttcgcttcca atgtggcccc gaaggctacg ttctcaaccc tcagtggata gattgtctgg
     4801 gccatatcgc cggtttcatc atgaacggaa atgactctgt tgcttccaga gacacagtct
     4861 tcgtgaatca tggttgggaa aatatgcaga tcgcaactaa gttctccgag gataagacat
     4921 atcggacata taacaagatg cagctgacca ccaacaatat ctatgttggg gacacataca
     4981 tccttgaggg tgacgaaata gttgccattt attctggcat aaagtttcaa ggtgtgcctc
     5041 gtcgtgtact cgaccagttg cttccgtctg ggcagcggcg agcaattcca gtcaccacaa
     5101 cacccgctcc caaacttcaa gaagccaaaa tttcaaatgc tctttctaag gggaagagta
     5161 tttcatcacc gaagcacaag gtgtcatcta ttaaagacac gattctcaat ctaattgccg
     5221 aggctgttgg ccttgatgtc aacgaactga aacccgacac gacctttgac gagtgcggag
     5281 ttgattcttt actctcactc aatatttcta aagcgatgca tgatcagctt gggctggata
     5341 tcaactccag tacatttgca gaatgtccaa caatcagctc tttcttgagc cgggttaaag
     5401 aaaacgaagc ttctccaggg actctaacac ctcaatcatc caattccagt actgccgatc
     5461 tcgatagtct tctggatgac aatataagtc tcgcgtcatc tattactagc gatgaagacg
     5521 agaaagagga gccacttgaa aagagcacca acaagactat cgagctcatc agatcagtta
     5581 ttgctgccga agttggactt gaaccatctg aaattggtga ttcgacaaat ttctcagacc
     5641 tcggcattga ctctttgctc agccttaaca ttatgagtcg tctagaggag attatcgaga
     5701 gcgagattcc aaggatgcta ctcgcggaaa atgagacact cgagaaggta gctcgcgcac
     5761 ttggtctgca gacaaccgta tcgaatgtga ctctggaaaa acaaaagcag aagctaagca
     5821 acaatacttc cgaaacaaag tttgcagtaa ggagtccacc gcatgcttct agccacctcc
     5881 tgcaaggtaa cgcgaagact gcgacgaaaa agctttttct ttttccagat ggttctggct
     5941 ctgccttttc ctacgcaagc ctctcaagga tatccccctc tgtggcggtc tatggccttg
     6001 catgtccatg gcagaaagat ccatggtcca tggccggcgt gtctttctct cagctcagcg
     6061 ccaaatttat cgcagctatc aaaaagatcc aaccatgtgg accatatcac ctcggtggct
     6121 ggtctgccgg tggaatcagt gcgtacgatg ctgcgcaaca gcttgctgcc gatggcgatg
     6181 ttgtagagag ccttgtactg attgactctc ccaatcccat tggactcgag aaccctccac
     6241 agcggatgta tgacttcttt gacgaacttg actttttcgg catggaaggc aaagcaccac
     6301 caacatggct ccgcgctcac tttgatgcct tccttacaac gcttgatgag tacaagattg
     6361 ctccatggaa acaaggaaac agcccagcta cgtacattgt atacgcaaaa aatggcgtgt
     6421 gcgacatgct gaagcctgag gatccgcgcc ccgagatcag ggatgacgac cctagggaaa
     6481 tgaagtggct actgaacacg cggactgact tttctgcgac aggctggaag agccttgttg
     6541 gagattgcaa tgtcagtgtt ggggttattg aaggagtcaa ccatttcagc atgatgaata
     6601 aggggcccaa gtcggatgag cttgcagact tcatcaagag tgcattgaag atgtagggag
     6661 attgataaga cttttctagt tgcatatctt ttatatttaa atcttatcta ttagttaatt
     6721 ttttgtaatt tatccttata tatagtctgg ttattctaaa atatcatttc agtatctaaa
     6781 aattcccctc ttttttcagt tatatcttaa caggcgacag tccaaatgtt gatttatccc
     6841 agtccgattc atcagggttg tgaagcattt tgtcaatggt cgaaatcaca tcagtaatag
     6901 tgcctcttac ttgcctcata gaatttcttt ctcttaacgt caccgtttgg tcttttatag
     6961 tttcgaaatc tatggtgata ccaaatggtg ttcccaattc atcgttacgg gcgtattttt
     7021 taccaattga agtattggaa tcgtcaattt taaagtatat ctctctttta cgtaaagcct
     7081 gcgagatcct cttaagtata gcggggaagc catcgttatt cgatattgtc gtaacaaata
     7141 ctttgatcgg cgctatgcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt
     7201 gagggttaat tgcgcgcttg gcgagactca aacaagatat attttgcccg ccttacagac
     7261 gaaactagtt ggaggttatg gagcatacta tcacgtgggc ggccactggt gagttactac
     7321 accccagggg caacgttgat gctcctaaaa aactctggct ggacgcaagc cgtaacaccc
     7381 gtgtcacttc ataatcgttt gcaattcagg gcttgatcta cacattaccc tgttatccct
     7441 attaattaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     7501 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     7561 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7621 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7681 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7741 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7801 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7861 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7921 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7981 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     8041 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     8101 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     8161 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     8221 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     8281 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     8341 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     8401 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     8461 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     8521 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     8581 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8641 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8701 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8761 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8821 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8881 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8941 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     9001 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     9061 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     9121 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     9181 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     9241 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     9301 aacgaagcat ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt
     9361 caaacaaaga atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt
     9421 taccaacgaa gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa
     9481 tttttcaaac aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc
     9541 tattttacca acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc
     9601 gctatttttc taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg
     9661 cagtctcttg ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg
     9721 tgtctatttt ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta
     9781 gcgaagctgc gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat
     9841 gtggattgcg catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag
     9901 aaaattatga acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca
     9961 ttttcgtatt gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa
    10021 gagtaatact agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg
    10081 agcgaaaggt ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga
    10141 tacttttgag caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc
    10201 cggtgcgttt ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc
    10261 tctgaagttc ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt
    10321 ccgaaaacga gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac
    10381 gtcgcaccta tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg
    10441 cgtgtttatg cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct
    10501 agtacctcct gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta
    10561 ccctttagct gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct
    10621 atcatttcct ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat
    10681 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgagcgc
    10741 gtttcggtga tgacggtgaa aacctcggcg cgccattacc ctgttatccc taatatacaa
    10801 cgcaaagaag ctggaaaatt ggcgagggaa tcctgtttct gtctatccaa gaatgggcat
    10861 gaggtggcaa ccgtcgtgct agcgtacagg gtgcactttg taaccatttg ggacaccgga
    10921 cactcgctgt tttcgaaatt accctttaag cgcgggtatt gaaccaggct tatgcccaag
    10981 atcgtagcaa gcatttaact gtgggaatac tcaggtatcg taagatgcaa gagttcgaat
    11041 ctcttagcaa ccattatttt tttcctcaac ataacgagaa cacacagggg cgctatcgca
    11101 cagaatcaaa ttcgatgact ggaaattttt tgttaatttc agaggtcgcc tgacgcatat
    11161 acctttttca actgaaaaat tgggagaaaa aggaaaggtg agagcgccgg aaccggcttt
    11221 tcatatagaa tagagaagcg ttcatgacta aatgcttgca tcacaatact tgaagttgac
    11281 aatattattt aaggacctat tgttttttcc aataggtggt tagcaatcgt cttactttct
    11341 aacttttctt accttttaca tttcagcaat atatatatat atatttcaag gatataccat
    11401 tctaatgtct gcccctaaga agatcgtcgt tttgccaggt gaccacgttg gtcaagaaat
    11461 cacagccgaa gccattaagg ttcttaaagc tatttctgat gttcgttcca atgtcaagtt
    11521 cgatttcgaa aatcatttaa ttggtggtgc tgctatcgat gctacaggtg ttccacttcc
    11581 agatgaggcg ctggaagcct ccaagaaggc tgatgccgtt ttgttaggtg ctgtgggtgg
    11641 tcctaaatgg ggtaccggta gtgttagacc tgaacaaggt ttactaaaaa tccgtaaaga
    11701 acttcaattg tacgccaact taagaccatg taactttgca tccgactctc ttttagactt
    11761 atctccaatc aagccacaat ttgctaaagg tactgacttc gttgttgtca gagaattagt
    11821 gggaggtatt tactttggta agagaaagga agacgatggt gatggtgtcg cttgggatag
    11881 tgaacaatac accgttccag aagtgcaaag aatcacaaga atggccgctt tcatggccct
    11941 acaacatgag ccaccattgc ctatttggtc cttggataaa gctaatgttt tggcctcttc
    12001 aagattatgg agaaaaactg tggaggaaac catcaagaac gaattcccta cattgaaggt
    12061 tcaacatcaa ttgattgatt ctgccgccat gatcctagtt aagaacccaa cccacctaaa
    12121 tggtattata atcaccagca acatgtttgg tgatatcatc tccgatgaag cctccgttat
    12181 cccaggttcc ttgggtttgt tgccatctgc gtccttggcc tctttgccag acaagaacac
    12241 cgcatttggt ttgtacgaac catgccacgg ttctgctcca gatttgccaa agaataaggt
    12301 caaccctatc gccactatct tgtctgctgc aatgatgttg aaattgtcat tgaacttgcc
    12361 tgaagaaggt aaggccattg aagatgcagt taaaaaggtt ttggatgcag gtatcagaac
    12421 tggtgattta ggtggttcca acagtaccac cgaagtcggt gatgctgtcg ccgaagaagt
    12481 taagaaaatc cttgcttaaa aagattctct ttttttatga tatttgtaca taaactttat
    12541 aaatgaaatt cataatagaa acgacacgaa attacaaaat ggaatatgtt catagggtag
    12601 acgaaactat atacgcaatc tacatacatt tatcaagaag gagaaaaagg aggatgtaaa
    12661 ggaatacagg taagcaaatt gatactaatg gctcaacgaa atgacggtat cgataagctt
    12721 gatatcgaat tcctgcagcc cgggggatcc actagttcta gattaattaa tatctaaaaa
    12781 ttgccttatg atccgtctct ccggttacag cctgtgtaac tgattaatcc tgcctttcta
    12841 atcaccattc taatgtttta attaagggat tttgtcttca ttaacggctt tcgctcataa
    12901 aaatgttatg acgttttgcc cgcaggcggg aaaccatcca cttcacgaga ctgatctcct
    12961 ctgccggaac accgggcatc tccaacttat aagttggaga aataagagaa tttcagattg
    13021 a
//
