LOCUS       sequence-225620-        2556 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in 
            mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Kuhnle S, Martinez-Noel G, Leclere F, Hayes SD, Harper JW, Howley PM
  TITLE     Angelman syndrome-associated point mutations in the Zn-binding 
            N-terminal (AZUL) domain of UBE3A ubiquitin ligase inhibit binding 
            to the proteasome.
  JOURNAL   J Biol Chem. 2018 Sep 26. pii: RA118.004653. doi: 
            10.1074/jbc.RA118.004653.
  PUBMED    30257870
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2018 Sep 26. pii: RA118.004653. doi: 
            10.1074/jbc.RA118.004653."
COMMENT     Sequence Label: PHAGE-P CMVt N-HA hE6-AP I (C21Y)
FEATURES             Location/Qualifiers
     source          1..2556
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgaagcgag cagctgcaaa gcatctaata gaacgctact accaccagtt aactgagggc
       61 tatggaaatg aagcctgcac gaatgagttt tgtgcttcct gtccaacttt tcttcgtatg
      121 gataataatg cagcagctat taaagccctc gagctttata agattaatgc aaaactctgt
      181 gatcctcatc cctccaagaa aggagcaagc tcagcttacc ttgagaactc gaaaggtgcc
      241 cccaacaact cctgctctga gataaaaatg aacaagaaag gcgctagaat tgattttaaa
      301 gatgtgactt acttaacaga agagaaggta tatgaaattc ttgaattatg tagagaaaga
      361 gaggattatt cccctttaat ccgtgttatt ggaagagttt tttctagtgc tgaggcattg
      421 gtacagagct tccggaaagt taaacaacac accaaggaag aactgaaatc tcttcaagca
      481 aaagatgaag acaaagatga agatgaaaag gaaaaagctg catgttctgc tgctgctatg
      541 gaagaagact cagaagcatc ttcctcaagg ataggtgata gctcacaggg agacaacaat
      601 ttgcaaaaat taggccctga tgatgtgtct gtggatattg atgccattag aagggtctac
      661 accagattgc tctctaatga aaaaattgaa actgcctttc tcaatgcact tgtatatttg
      721 tcacctaacg tggaatgtga cttgacgtat cacaatgtat actctcgaga tcctaattat
      781 ctgaatttgt tcattatcgt aatggagaat agaaatctcc acagtcctga atatctggaa
      841 atggctttgc cattattttg caaagcgatg agcaagctac cccttgcagc ccaaggaaaa
      901 ctgatcagac tgtggtctaa atacaatgca gaccagattc ggagaatgat ggagacattt
      961 cagcaactta ttacttataa agtcataagc aatgaattta acagtcgaaa tctagtgaat
     1021 gatgatgatg ccattgttgc tgcttcgaag tgcttgaaaa tggtttacta tgcaaatgta
     1081 gtgggagggg aagtggacac aaatcacaat gaagaagatg atgaagagcc catccctgag
     1141 tccagcgagc tgacacttca ggaacttttg ggagaagaaa gaagaaacaa gaaaggtcct
     1201 cgagtggacc ccctggaaac tgaacttggt gttaaaaccc tggattgtcg aaaaccactt
     1261 atcccttttg aagagtttat taatgaacca ctgaatgagg ttctagaaat ggataaagat
     1321 tatacttttt tcaaagtaga aacagagaac aaattctctt ttatgacatg tccctttata
     1381 ttgaatgctg tcacaaagaa tttgggatta tattatgaca atagaattcg catgtacagt
     1441 gaacgaagaa tcactgttct ctacagctta gttcaaggac agcagttgaa tccatatttg
     1501 agactcaaag ttagacgtga ccatatcata gatgatgcac ttgtccggct agagatgatc
     1561 gctatggaaa atcctgcaga cttgaagaag cagttgtatg tggaatttga aggagaacaa
     1621 ggagttgatg agggaggtgt ttccaaagaa ttttttcagc tggttgtgga ggaaatcttc
     1681 aatccagata ttggtatgtt cacatacgat gaatctacaa aattgttttg gtttaatcca
     1741 tcttcttttg aaactgaggg tcagtttact ctgattggca tagtactggg tctggctatt
     1801 tacaataact gtatactgga tgtacatttt cccatggttg tctacaggaa gctaatgggg
     1861 aaaaaaggaa cttttcgtga cttgggagac tctcacccag ttctatatca gagtttaaaa
     1921 gatttattgg agtatgaagg gaatgtggaa gatgacatga tgatcacttt ccagatatca
     1981 cagacagatc tttttggtaa cccaatgatg tatgatctaa aggaaaatgg tgataaaatt
     2041 ccaattacaa atgaaaacag gaaggaattt gtcaatcttt attctgacta cattctcaat
     2101 aaatcagtag aaaaacagtt caaggctttt cggagaggtt ttcatatggt gaccaatgaa
     2161 tctcccttaa agtacttatt cagaccagaa gaaattgaat tgcttatatg tggaagccgg
     2221 aatctagatt tccaagcact agaagaaact acagaatatg acggtggcta taccagggac
     2281 tctgttctga ttagggagtt ctgggaaatc gttcattcat ttacagatga acagaaaaga
     2341 ctcttcttgc agtttacaac gggcacagac agagcacctg tgggaggact aggaaaatta
     2401 aagatgatta tagccaaaaa tggcccagac acagaaaggt tacctacatc tcatacttgc
     2461 tttaatgtgc ttttacttcc ggaatactca agcaaagaaa aacttaaaga gagattgttg
     2521 aaggccatca cgtatgccaa aggatttggc atgctg
//
