LOCUS       sequence-225635-       10927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human MCM2 and human MCM7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10927)
  AUTHORS   Hesketh EL, Parker-Manuel RP, Chaban Y, Satti R, Coverley D, Orlova 
            EV, Chong JP
  TITLE     DNA induces conformational changes in a recombinant human 
            minichromosome maintenance complex.
  JOURNAL   J Biol Chem. 2015 Mar 20;290(12):7973-9. doi: 
            10.1074/jbc.M114.622738. Epub 2015 Feb 3.
  PUBMED    25648893
REFERENCE   2  (bases 1 to 10927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M114.622738"; journalName: "J Biol Chem"; date: 
            "2015-03-20- 20"; volume: "290"; issue: "12"; pages: "7973-9"
COMMENT     Sequence Label: pET32-MCM2/7
FEATURES             Location/Qualifiers
     source          1..10927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        493..597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(598..666,667..1458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(816..835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1629..2217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2118..2137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2371..2388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2403..2542
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2468..2487)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2628..2650
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2644..2835)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3644..4726)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4688..4707
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4727..4804)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     5010..5029
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5117..5135
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    5136..5160
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             5175..5197
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5205..5531
                     /codon_start=1
                     /gene="trxA"
                     /product="E. coli thioredoxin"
                     /label=TrxA
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILDE
                     IADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKEFL
                     DANLA"
     CDS             5553..5570
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             5580..5597
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             5604..5648
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             5664..5678
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     promoter        7872..7890
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    7891..7915
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             7930..7952
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             10771..10788
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(10841..10859)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10855..10902
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
      601 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
      661 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
      721 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
      781 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      841 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      901 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      961 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     1021 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     1081 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     1141 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     1201 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     1261 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     1321 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     1381 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     1441 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     1501 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     1561 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     1621 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     1681 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1741 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     1801 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1861 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1921 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1981 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     2041 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     2101 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     2161 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     2221 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     2281 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     2341 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     2401 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg caatggtgca
     2461 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     2521 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     2581 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     2641 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     2701 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     2761 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     2821 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     2881 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     2941 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     3001 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
     3061 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     3121 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     3181 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     3241 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     3301 tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
     3361 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     3421 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     3481 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     3541 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
     3601 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
     3661 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     3721 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     3781 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     3841 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     3901 tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg
     3961 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     4021 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     4081 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     4141 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     4201 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     4261 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     4321 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     4381 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     4441 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     4501 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     4561 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     4621 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     4681 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     4741 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     4801 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     4861 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
     4921 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     4981 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     5041 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgat
     5101 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
     5161 cctctagaaa taattttgtt taactttaag aaggagatat acatatgagc gataaaatta
     5221 ttcacctgac tgacgacagt tttgacacgg atgtactcaa agcggacggg gcgatcctcg
     5281 tcgatttctg ggcagagtgg tgcggtccgt gcaaaatgat cgccccgatt ctggatgaaa
     5341 tcgctgacga atatcagggc aaactgaccg ttgcaaaact gaacatcgat caaaaccctg
     5401 gcactgcgcc gaaatatggc atccgtggta tcccgactct gctgctgttc aaaaacggtg
     5461 aagtggcggc aaccaaagtg ggtgcactgt ctaaaggtca gttgaaagag ttcctcgacg
     5521 ctaacctggc cggttctggt tctggccata tgcaccatca tcatcatcat tcttctggtc
     5581 tggtgccacg cggttctggt atgaaagaaa ccgctgctgc taaattcgaa cgccagcaca
     5641 tggacagccc agatctgggt accgatgacg acgacaagat ggcactgaag gactacgcgc
     5701 tagagaagga aaaggttaag aagttcttac aagagttcta ccaggatgat gaactcggga
     5761 agaagcagtt caagtatggg aaccagttgg ttcggctggc tcatcgggaa caggtggctc
     5821 tgtatgtgga cctggacgac gtagccgagg atgaccccga gttggtggac tcaatttgtg
     5881 agaatgccag gcgctacgcg aagctctttg ctgatgccgt acaagagctg ctgcctcagt
     5941 acaaggagag ggaagtggta aataaagatg tcctggacgt ttacattgag catcggctaa
     6001 tgatggagca gcggagtcgg gaccctggga tggtccgaag cccccagaac cagtaccctg
     6061 ctgaactcat gcgcagattt gagctgtatt ttcaaggccc tagcagcaac aagcctcgtg
     6121 tgatccggga agtgcgggct gactctgtgg ggaagttggt aactgtgcgt ggaatcgtca
     6181 ctcgtgtctc tgaagtcaaa cccaagatgg tggtggccac ttacacttgt gaccagtgtg
     6241 gggcagagac ctaccagccg atccagtctc ccactttcat gcctctgatc atgtgcccaa
     6301 gccaggagtg ccaaaccaac cgctcaggag ggcggctgta tctgcagaca cggggctcca
     6361 gattcatcaa attccaggag atgaagatgc aagaacatag tgatcaggtg cctgtgggaa
     6421 atatccctcg tagtatcacg gtgctggtag aaggagagaa cacaaggatt gcccagcctg
     6481 gagaccacgt cagcgtcact ggtattttct tgccaatcct gcgcactggg ttccgacagg
     6541 tggtacaggg tttactctca gaaacctacc tggaagccca tcggattgtg aagatgaaca
     6601 agagtgagga tgatgagtct ggggctggag agctcaccag ggaggagctg aggcaaattg
     6661 cagaggagga tttctacgaa aagctggcag cttcaatcgc cccagaaata tacgggcatg
     6721 aagatgtgaa gaaggcactg ctgctcctgc tagtcggggg tgtggaccag tctcctcgag
     6781 gcatgaaaat ccggggcaac atcaacatct gtctgatggg ggatcctggt gtggccaagt
     6841 ctcagctcct gtcatacatt gatcgactgg cgcctcgcag ccagtacaca acaggccggg
     6901 gctcctcagg agtggggctt acggcagctg tgctgagaga ctccgtgagt ggagaactga
     6961 ccttagaggg tggggccctg gtgctggctg accagggtgt gtgctgcatt gatgagttcg
     7021 acaagatggc tgaggccgac cgcacagcca tccacgaggt catggagcag cagaccatct
     7081 ccattgccaa ggccggcatt ctcaccacac tcaatgcccg ctgctccatc ctggctgccg
     7141 ccaaccctgc ctacgggcgc tacaaccctc gccgcagcct ggagcagaac atacagctac
     7201 ctgctgcact gctctcccgg tttgacctcc tctggctgat tcaggaccgg cccgaccgag
     7261 acaatgacct acggttggcc cagcacatca cctatgtgca ccagcacagc cggcagcccc
     7321 cctcccagtt tgaacctctg gacatgaagc tcatgaggcg ttacatagcc atgtgccgcg
     7381 agaagcagcc catggtgcca gagtctctgg ctgactacat cacagcagca tacgtggaga
     7441 tgaggcgaga ggcttgggct agtaaggatg ccacctatac ttctgcccgg accctgctgg
     7501 ctatcctgcg cctttccact gctctggcac gtctgagaat ggtggatgtg gtggagaaag
     7561 aagatgtgaa tgaagccatc aggctaatgg agatgtcaaa ggactctctt ctaggagaca
     7621 aggggcagac agctaggact cagagaccag cagatgtgat atttgccacc gtccgtgaac
     7681 tggtctcagg gggccgaagt gtccggttct ctgaggcaga gcagcgctgt gtatctcgtg
     7741 gcttcacacc cgcccagttc caggcggctc tggatgaata tgaggagctc aatgtctggc
     7801 aggtcaatgc ttcccggaca cggatcactt ttgtctgacg gccgcccgcg ttgatctcga
     7861 tcccgcgaaa ttaatacgac tcactatagg ggaattgtga gcggataaca attcccctct
     7921 agaaataatt ttgtttaact ttaagaagga gatataccat gcaagcgggc ccggcctcca
     7981 tggcggaatc atcggaatcc ttcaccatgg catccagccc ggcccagcgt cggcgaggca
     8041 atgatcctct cacctccagc cctggccgaa gctcccggcg tactgatgcc ctcacctcca
     8101 gccctggccg tgaccttcca ccatttgagg atgagtccga ggggctccta ggcacagagg
     8161 ggcccctgga ggaagaagag gatggagagg agctcattgg agatggcatg gaaagggact
     8221 accgcgccat cccagagctg gacgcctatg aggccgaggg actggctctg gatgatgagg
     8281 acgtagagga gctgacggcc agtcagaggg aggcagcaga gcgggccatg cggcagcgtg
     8341 accgggaggc tggccggggc ctgggccgca tgcgccgtgg gctcctgtat gacagcgatg
     8401 aggaggacga ggagcgccct gcccgcaagc gccgccaggt ggagcgggcc acggaggacg
     8461 gcgaggagga cgaggagatg attgagagca tcgagaacct ggaggatctc aaaggccact
     8521 ctgtgcgcga gtgggtgagc atggcgggcc cccggctgga gatccaccac cgcttcaaga
     8581 acttcctgcg cactcacgtc gacagccacg gccacaacgt cttcaaggag cgcatcagcg
     8641 acatgtgcaa agagaaccgt gagagcctgg tggtgaacta tgaggacttg gcagccaggg
     8701 agcacgtgct ggcctacttc ctgcctgagg caccggcgga gctgctgcag atctttgatg
     8761 aggctgccct ggaggtggta ctggccatgt accccaagta cgaccgcatc accaaccaca
     8821 tccatgtccg catctcccac ctgcctctgg tggaggagct gcgctcgctg aggcagctgc
     8881 atctgaacca gctgatccgc accagtgggg tggtgaccag ctgcactggc gtcctgcccc
     8941 agctcagcat ggtcaagtac aactgcaaca agtgcaattt cgtcctgggt cctttctgcc
     9001 agtcccagaa ccaggaggtg aaaccaggct cctgtcctga gtgccagtcg gccggcccct
     9061 ttgaggtcaa catggaggag accatctatc agaactacca gcgtatccga atccaggaga
     9121 gtccaggcaa agtggcggct ggccggctgc cccgctccaa ggacgccatt ctcctcgcag
     9181 atctggtgga cagctgcaag ccaggagacg agatagagct gactggcatc tatcacaaca
     9241 actatgatgg ctccctcaac actgccaatg gcttccctgt ctttgccact gtcatcctag
     9301 ccaaccacgt ggccaagaag gacaacaagg ttgctgtagg ggaactgacc gatgaagatg
     9361 tgaagatgat cactagcctc tccaaggatc agcagatcgg agagaagatc tttgccagca
     9421 ttgctccttc catctatggt catgaagaca tcaagagagg cctggctctg gccctgttcg
     9481 gaggggagcc caaaaaccca ggtggcaagc acaaggtacg tggtgatatc aacgtgctct
     9541 tgtgcggaga ccctggcaca gcgaagtcgc agtttctcaa gtatattgag aaagtgtcca
     9601 gccgagccat cttcaccact ggccaggggg cgtcggctgt gggcctcacg gcgtatgtcc
     9661 agcggcaccc tgtcagcagg gagtggacct tggaggctgg ggccctggtt ctggctgacc
     9721 gaggagtgtg tctcattgat gaatttgaca agatgaatga ccaggacaga accagcatcc
     9781 atgaggccat ggagcaacag agcatctcca tctcgaaggc tggcatcgtc acctccctgc
     9841 aggctcgctg cacggtcatt gctgccgcca accccatagg agggcgctac gacccctcgc
     9901 tgactttctc tgagaacgtg gacctcacag agcccatcat ctcacgcttt gacatcctgt
     9961 gtgtggtgag ggacaccgtg gacccagtcc aggacgagat gctggcccgc ttcgtggtgg
    10021 gcagccacgt cagacaccac cccagcaaca aggaggagga ggggctggcc aatggcagcg
    10081 ctgctgagcc cgccatgccc aacacgtatg gcgtggagcc cctgccccag gaggtcctga
    10141 agaagtacat catctacgcc aaggagaggg tccacccgaa gctcaaccag atggaccagg
    10201 acaaggtggc caagatgtac agtgacctga ggaaagaatc tatggcgaca ggcagcatcc
    10261 ccattacggt gcggcacatc gagtccatga tccgcatggc ggaggcccac gcgcgcatcc
    10321 atctgcggga ctatgtgatc gaagacgacg tcaacatggc catccgcgtg atgctggaga
    10381 gcttcataga cacacagaag ttcagcgtca tgcgcagcat gcgcaagact tttgcccgct
    10441 acctttcatt ccggcgtgac aacaatgagc tgttgctctt catactgaag cagttagtgg
    10501 cagagcaggt gacatatcag cgcaaccgct ttggggccca gcaggacact attgaggtcc
    10561 ctgagaagga cttggtggat aaggctcgtc agatcaacat ccacaacctc tctgcatttt
    10621 atgacagtga gctcttcagg atgaacaagt tcagccacga cctgaaaagg aaaatgatcc
    10681 tgcagcagtt ctgacccggg cttctcctca accatggcga tatcggatcc gaattcgagc
    10741 tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg agatccggct
    10801 gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca
    10861 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata
    10921 tccggat
//
