LOCUS       sequence-225659-        9908 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  drives expression of V5-NES-APEX2-GFP from human PGK promotor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9908)
  AUTHORS   Markmiller S, Soltanieh S, Server KL, Mak R, Jin W, Fang MY, Luo EC,
            Krach F, Yang D, Sen A, Fulzele A, Wozniak JM, Gonzalez DJ, Kankel 
            MW, Gao FB, Bennett EJ, Lecuyer E, Yeo GW
  TITLE     Context-Dependent and Disease-Specific Diversity in Protein 
            Interactions within Stress Granules.
  JOURNAL   Cell. 2018 Jan 25;172(3):590-604.e13. doi: 
            10.1016/j.cell.2017.12.032.
  PUBMED    29373831
REFERENCE   2  (bases 1 to 9908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2017.12"; journalName: "Cell"; date: "2018-01-25- 
            25"; volume: "172"; issue: "3"; pages: "590-604.e13"
COMMENT     Sequence Label: hPGK_V5-APEX2-GFP
FEATURES             Location/Qualifiers
     source          1..9908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        403..903
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     822..840
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             913..954
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             955..1701
                     /codon_start=1
                     /product="soybean ascorbate peroxidase, engineered to serve
                     as a monomeric tag for electron microscopy and live-cell 
                     proteomics (Lam et al., 2015)"
                     /label=APEX2
                     /note="more active than the original APEX due to the A134P 
                     mutation"
                     /translation="GKSYPTVSADYQDAVEKAKKKLRGFIAEKRCAPLMLRLAFHSAGT
                     FDKGTKTGGPFGTIKHPAELAHSANNGLDIAVRLLEPLKAEFPILSYADFYQLAGVVAV
                     EVTGGPKVPFHPGREDKPEPPPEGRLPDPTKGSDHLRDVFGKAMGLTDQDIVALSGGHT
                     IGAAHKERSGFEGPWTSNPLIFDNSYFTELLSGEKEGLLQLPSDKALLSDPVFRPLVDK
                     YAADEDAFFADYAEAHQKLSELGFADA"
     CDS             1708..1734
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             1792..2454
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina 
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /label=CopGFP
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
     misc_feature    2545..3133
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(3016..3027)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3207..3440
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3512..3633
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(3720..3753)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        4107..4318
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             4331..4599
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     polyA_signal    5356..5477
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5977..6010)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        6068..6567
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6103..6126)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6500..6519
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6594..7166
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          7243..7308
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(7376..7393)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7382..7606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(7687..7703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7687..7703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7700..7722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7711..7727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7735..7741,7742..7759,7760..7765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7780..7801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7918..7935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8089..8677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8848..9639,9640..9708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9471..9490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9709..9813)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9881..9899
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgttgg ggttgcgcct tttccaaggc
      421 agccctgggt ttgcgcaggg acgcggctgc tctgggcgtg gttccgggaa acgcagcggc
      481 gccgaccctg ggtctcgcac attcttcacg tccgttcgca gcgtcacccg gatcttcgcc
      541 gctacccttg tgggcccccc ggcgacgctt cctgctccgc ccctaagtcg ggaaggttcc
      601 ttgcggttcg cggcgtgccg gacgtgacaa acggaagccg cacgtctcac tagtaccctc
      661 gcagacggac agcgccaggg agcaatggca gcgcgccgac cgcgatgggc tgtggccaat
      721 agcggctgct cagcagggcg cgccgagagc agcggccggg aaggggcggt gcgggaggcg
      781 gggtgtgggg cggtagtgtg ggccctgttc ctgcccgcgc ggtgttccgc attctgcaag
      841 cctccggagc gcacgtcggc agtcggctcc ctcgttgacc gaatcaccga cctctctccc
      901 caggaattca tgggtaagcc tatccctaac cctctcctcg gtctcgattc tacgggaaag
      961 tcttacccaa ctgtgagtgc tgattaccag gacgccgttg agaaggcgaa gaagaagctc
     1021 agaggcttca tcgctgagaa gagatgcgct cctctaatgc tccgtttggc attccactct
     1081 gctggaacct ttgacaaggg cacgaagacc ggtggaccct tcggaaccat caagcaccct
     1141 gccgaactgg ctcacagcgc taacaacggt cttgacatcg ctgttaggct tttggagcca
     1201 ctcaaggcgg agttccctat tttgagctac gccgatttct accagttggc tggcgttgtt
     1261 gccgttgagg tcacgggtgg acctaaggtt ccattccacc ctggaagaga ggacaagcct
     1321 gagccaccac cagagggtcg cttgcccgat cccactaagg gttctgacca tttgagagat
     1381 gtgtttggca aagctatggg gcttactgac caagatatcg ttgctctatc tgggggtcac
     1441 actattggag ctgcacacaa ggagcgttct ggatttgagg gtccctggac ctctaatcct
     1501 cttattttcg acaactcata cttcacggag ttgttgagtg gtgagaagga aggtctcctt
     1561 cagctacctt ctgacaaggc tcttttgtct gaccctgtat tccgccctct cgttgacaaa
     1621 tatgcagcgg acgaagatgc cttctttgct gattacgctg aggctcacca aaagctttcc
     1681 gagcttgggt ttgctgatgc cctgcagctg cctcccctgg agcgcctgac cctggacggt
     1741 ggcggtggct cgggcggtgg tgggtcgatg gagagcgacg agagcggcct gcccgccatg
     1801 gagatcgagt gccgcatcac cggcaccctg aacggcgtgg agttcgagct ggtgggcggc
     1861 ggagagggca cccccaagca gggccgcatg accaacaaga tgaagagcac caaaggcgcc
     1921 ctgaccttca gcccctacct gctgagccac gtgatgggct acggcttcta ccacttcggc
     1981 acctacccca gcggctacga gaaccccttc ctgcacgcca tcaacaacgg cggctacacc
     2041 aacacccgca tcgagaagta cgaggacggc ggcgtgctgc acgtgagctt cagctaccgc
     2101 tacgaggccg gccgcgtgat cggcgacttc aaggtggtgg gcaccggctt ccccgaggac
     2161 agcgtgatct tcaccgacaa gatcatccgc agcaacgcca ccgtggagca cctgcacccc
     2221 atgggcgata acgtgctggt gggcagcttc gcccgcacct tcagcctgcg cgacggcggc
     2281 tactacagct tcgtggtgga cagccacatg cacttcaaga gcgccatcca ccccagcatc
     2341 ctgcagaacg ggggccccat gttcgccttc cgccgcgtgg aggagctgca cagcaacacc
     2401 gagctgggca tcgtggagta ccagcacgcc ttcaagaccc ccatcgcctt cgccagatcc
     2461 cgcgctcagt cgtccaattc tgccgtggac ggcaccgccg gacccggctc caccggatct
     2521 cgctagagct gaatctaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     2581 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     2641 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     2701 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     2761 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     2821 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     2881 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     2941 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     3001 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     3061 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     3121 gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     3181 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga
     3241 tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     3301 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     3361 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     3421 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     3481 tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta
     3541 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     3601 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc
     3661 ctaactaaaa ttcgagctcg gtacctcgcg aatgcatcag atctttccac tcaaaacata
     3721 taacttcgta tagcatacat tatacgaagt tatattcaaa aatctagaca gcggccgcca
     3781 ctcaaaattt cttctataaa gtaacaaaac ttttatgagg gacagccccc ccccaaagcc
     3841 cccagggatg taattacgtc cctcccccgc tagggggcag cagcgagccg cccggggctc
     3901 cgctccggtc cggcgctccc cccgcatccc cgagccggca gcgtgcgggg acagcccggg
     3961 cacggggaag gtggcacggg atcgctttcc tctgaacgct tctcgctgct ctttgagcct
     4021 gcagacacct ggggggatac ggggaaaagg cctccaaggc cttaattcgg ccgcaaggat
     4081 ctgcgatcgc tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa
     4141 gttgggggga ggggtcggca attgaacggg tgcctagaga aggtggcgcg gggtaaactg
     4201 ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat
     4261 aagtgcagta gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacagct
     4321 gaagcttcga ggggctcgca tctctccttc acgcgcccgc cgccctacct gaggccgcca
     4381 tccacgccgg ttgagtcgcg ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc
     4441 cgccgtctag gtaagtttaa agctcaggtc gagaccgggc ctttgtccgg cgctcccttg
     4501 gagcctacct agactcagcc ggctctccac gctttgcctg accctgcttg ctcaactcta
     4561 cgtctttgtt tcgttttctg ttctgcgccg ttacagatcc aagctgtgac cggcgcctac
     4621 gatatcgcca ccatgacgga gtataagccg acagttaggc ttgctactag ggacgatgtg
     4681 ccccgcgccg tccgcacact ggcagcagct tttgctgact accctgcaac acggcacacg
     4741 gtggacccgg acaggcatat cgagagggta acagagctgc aggagctgtt cttgacgcgg
     4801 gtaggtctgg atatcggtaa ggtttgggtg gcagacgacg gagcagctgt cgcggtgtgg
     4861 accaccccgg agagcgttga ggcgggagcg gttttcgccg aaatcgggcc taggatggct
     4921 gagttgtctg ggtccagact ggccgctcaa cagcaaatgg agggcttgct ggcaccgcat
     4981 cgcccgaaag agccagcttg gttcctggca accgtgggcg tttcacccga tcaccaaggc
     5041 aagggtctgg gaagtgcagt ggtgctgccc ggagtggaag ccgcagaacg cgcgggggtg
     5101 cccgcatttc tggagacaag tgcccctagg aacctgccct tctatgagag acttggtttc
     5161 accgttacag ctgacgtgga ggtgcccgag ggtccccgca cgtggtgcat gactcggaaa
     5221 ccgggcgctt gaaatcaacc tctggacgaa attccgcggt tattacaaaa tttgtgaaag
     5281 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     5341 gcctttgtat cagttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     5401 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     5461 ctcatcaatg tatcttatca tgtctggaat tgactcaaat gatgtcaatt agtctatcag
     5521 aagctatctg gtctcccttc cgggggacaa gacatccctg tttaatattt aaacagcagt
     5581 gttcccaaac tgggttctta tatcccttgc tctggtcaac caggttgcag ggtttcctgt
     5641 cctcacagga acgaagtccc taaagaaaca gtggcagcca ggtttagccc cggaattgac
     5701 tggattcctt ttttagggcc cattggtatg gctttttccc cgtatccccc caggtgtctg
     5761 caggctcaaa gagcagcgag aagcgttcag aggaaagcga tcccgtgcca ccttccccgt
     5821 gcccgggctg tccccgcacg ctgccggctc ggggatgcgg ggggagcgcc ggaccggagc
     5881 ggagccccgg gcggctcgct gctgccccct agcgggggag ggacgtaatt acatccctgg
     5941 gggctttggg ggggggctgt ccctgatatc aaacatataa cttcgtatag catacattat
     6001 acgaagttat tttatcacgt aagtagaaca tgaaataacc tagatcggat ccccgtcgac
     6061 tgcatgcggg taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc
     6121 ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc
     6181 ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc
     6241 ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa
     6301 gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta
     6361 ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg
     6421 ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga
     6481 aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc
     6541 tcctcttcct catctccggg cctttcgacc tgcaggtcct cgccatggat cctgatgatg
     6601 ttgttgattc ttctaaatct tttgtgatgg aaaacttttc ttcgtaccac gggactaaac
     6661 ctggttatgt agattccatt caaaaaggta tacaaaagcc aaaatctggt acacaaggaa
     6721 attatgacga tgattggaaa gggttttata gtaccgacaa taaatacgac gctgcgggat
     6781 actctgtaga taatgaaaac ccgctctctg gaaaagctgg aggcgtggtc aaagtgacgt
     6841 atccaggact gacgaaggtt ctcgcactaa aagtggataa tgccgaaact attaagaaag
     6901 agttaggttt aagtctcact gaaccgttga tggagcaagt cggaacggaa gagtttatca
     6961 aaaggttcgg tgatggtgct tcgcgtgtag tgctcagcct tcccttcgct gaggggagtt
     7021 ctagcgttga atatattaat aactgggaac aggcgaaagc gttaagcgta gaacttgaga
     7081 ttaattttga aacccgtgga aaacgtggcc aagatgcgat gtatgagtat atggctcaag
     7141 cctgtgcagg aaatcgtgtc aggcgatctc tttgtgaagg aaccttactt ctgtggtgtg
     7201 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
     7261 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
     7321 ggaactgatg aatgggagca gtggtggaat gcagatccta gagctcgctg atcagcctcg
     7381 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     7441 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     7501 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     7561 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     7621 agaaccagct ggggctcgag atccactagt tctagcctcg aggcatgcaa gcttggcgta
     7681 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     7741 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     7801 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     7861 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     7921 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     7981 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8041 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8101 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8161 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8221 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8281 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     8341 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     8401 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     8461 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     8521 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     8581 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     8641 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     8701 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     8761 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     8821 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     8881 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     8941 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9001 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9061 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9121 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9181 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9241 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9301 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     9361 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     9421 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     9481 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     9541 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     9601 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     9661 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     9721 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     9781 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     9841 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     9901 ctttcgtc
//
